FastQCFastQC Report
Thu 2 Feb 2017
SRR4062052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029760
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5750.027.1725061
GTATCAA13150.025.101791
TATACCG551.5936454E-423.9994435
GTCGTAA703.2153308E-521.9994930
GTCCTAT2300.018.1787871
AACCGCG1408.366078E-817.28615441
ACGGTAT2550.017.2545019
TCCTATT2450.017.0608272
TACGACG2850.016.9820635
GTAATAC1053.774219E-516.7615173
GTGTATA1753.610694E-916.3472421
GCGCAAT1501.8362698E-716.13374335
CGCAATA1501.8362698E-716.13374336
CTAGCGG1501.8372884E-716.1329629
GACGGTA2800.015.71392258
CTATTCC2403.6379788E-1215.5829724
ATCAACG21050.015.5720843
CTACGAC3250.015.56887154
CCTTATA850.00297999915.5290532
GCACCGT850.00297999915.5290536
Produced by FastQC (version 0.11.5)