##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062052_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1029760
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285689869484152	32.0	32.0	32.0	32.0	32.0
2	31.440270548477315	32.0	32.0	32.0	32.0	32.0
3	31.568839341205717	32.0	32.0	32.0	32.0	32.0
4	31.64426079863269	32.0	32.0	32.0	32.0	32.0
5	31.61072677128651	32.0	32.0	32.0	32.0	32.0
6	35.258604917650715	36.0	36.0	36.0	36.0	36.0
7	35.26256797700435	36.0	36.0	36.0	36.0	36.0
8	35.208970051274086	36.0	36.0	36.0	36.0	36.0
9	35.30040106432567	36.0	36.0	36.0	36.0	36.0
10	35.18200551584835	36.0	36.0	36.0	36.0	36.0
11	35.29525520509633	36.0	36.0	36.0	36.0	36.0
12	35.223865755127406	36.0	36.0	36.0	36.0	36.0
13	35.25686956183965	36.0	36.0	36.0	36.0	36.0
14	35.218088680857676	36.0	36.0	36.0	36.0	36.0
15	35.19709446861405	36.0	36.0	36.0	36.0	36.0
16	35.20771538999379	36.0	36.0	36.0	36.0	36.0
17	35.19026472187694	36.0	36.0	36.0	36.0	36.0
18	35.18475275792418	36.0	36.0	36.0	36.0	36.0
19	35.182874650403974	36.0	36.0	36.0	36.0	36.0
20	35.18045952454941	36.0	36.0	36.0	36.0	36.0
21	35.1748640459913	36.0	36.0	36.0	36.0	36.0
22	35.15298030609074	36.0	36.0	36.0	36.0	36.0
23	35.12065141392169	36.0	36.0	36.0	36.0	36.0
24	35.11473353014295	36.0	36.0	36.0	36.0	36.0
25	35.10369309353636	36.0	36.0	36.0	36.0	36.0
26	35.04910950124301	36.0	36.0	36.0	36.0	36.0
27	35.04030356587943	36.0	36.0	36.0	36.0	36.0
28	35.02412795214419	36.0	36.0	36.0	36.0	36.0
29	35.00312597110006	36.0	36.0	36.0	36.0	36.0
30	34.990944491920445	36.0	36.0	36.0	36.0	36.0
31	34.987750543816034	36.0	36.0	36.0	36.0	36.0
32	34.959807139527655	36.0	36.0	36.0	36.0	36.0
33	34.94002388906153	36.0	36.0	36.0	36.0	36.0
34	34.93152482131759	36.0	36.0	36.0	36.0	36.0
35	34.91826930546924	36.0	36.0	36.0	36.0	36.0
36	34.89133196084524	36.0	36.0	36.0	32.0	36.0
37	34.88679789465507	36.0	36.0	36.0	32.0	36.0
38	34.8710922933499	36.0	36.0	36.0	32.0	36.0
39	34.8517703154133	36.0	36.0	36.0	32.0	36.0
40	34.840942549720324	36.0	36.0	36.0	32.0	36.0
41	34.812168854878806	36.0	36.0	36.0	32.0	36.0
42	34.78892071939092	36.0	36.0	36.0	32.0	36.0
43	34.77555061373524	36.0	36.0	36.0	32.0	36.0
44	34.74269635643257	36.0	36.0	36.0	32.0	36.0
45	34.727319958048476	36.0	36.0	36.0	32.0	36.0
46	34.71063063238036	36.0	36.0	36.0	32.0	36.0
47	34.68832640615289	36.0	36.0	36.0	32.0	36.0
48	34.666656308266006	36.0	36.0	36.0	32.0	36.0
49	34.643932566811685	36.0	36.0	36.0	32.0	36.0
50	34.22617017557489	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	4.0
21	15.0
22	59.0
23	200.0
24	511.0
25	1122.0
26	2386.0
27	4392.0
28	7835.0
29	12416.0
30	18967.0
31	28047.0
32	43220.0
33	73426.0
34	168759.0
35	668399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23978085172521	17.626865091700342	11.999533727074914	26.133820329499535
2	16.673027711820374	19.090419100616195	37.1761815277191	27.060371659844325
3	19.423670975441826	22.12964392639443	27.814274990556044	30.632410107607704
4	13.11588439625194	15.412635383618886	33.77965135531712	37.69182886481206
5	15.185091671845866	35.99897063393412	32.61866842759478	16.197269266625234
6	36.20245961179153	33.653117825970426	15.880849443945944	14.2635731182921
7	32.03814481044127	29.340914387818522	18.977237414543193	19.643703387197018
8	28.710573337476692	33.20783483530143	18.02322871348664	20.05836311373524
9	26.734870261031695	14.843847110006214	18.43458669981355	39.98669592914854
10	17.453986806621742	26.270612832711343	29.65150098226867	26.623899378398246
11	37.51446939092604	21.30525559353636	21.077241298943445	20.103033716594158
12	24.9483374766936	23.226868396519578	27.771325357364823	24.053468769422
13	29.903861093847112	19.074056090739592	24.620299875699192	26.401782939714106
14	23.749417339962708	19.15553138595401	24.354509788688627	32.74054148539465
15	25.62558266003729	26.588816811684275	21.50510798632691	26.28049254195152
16	26.339545832394595	25.54391737039598	22.869972575921192	25.24656422128823
17	24.64030453697949	25.83572871348664	24.342759477936607	25.181207271597266
18	25.21599714107864	24.795995956335414	25.290674808377496	24.697332094208452
19	26.01472771784466	24.690631497272662	24.745110263663634	24.549530521219044
20	26.341482836710835	24.038704314420116	24.489490714305138	25.130322134563915
21	26.785173244251087	23.909454630205097	24.36820229956495	24.93716982597887
22	26.34982908638906	23.823609384711002	24.667204008701056	25.15935752019888
23	25.157706650093225	23.843905376009943	25.1323609384711	25.86602703542573
24	24.97149793639233	24.44661325564457	24.9773245933479	25.6045642146152
25	25.27581696299814	24.040867814702274	25.012163040089963	25.67115218220962
26	24.946831961808353	24.82690040533953	25.225539303561824	25.000728329290293
27	25.431655654349182	24.300805239299407	24.851615334117984	25.415923772233423
28	25.29145848203664	24.22535895157492	24.99283796316528	25.490344603223157
29	25.015489196406893	24.705802379218255	25.13192522456907	25.14678319980578
30	25.041757951490855	24.692059385633712	25.130129430227317	25.136053232648116
31	25.481307544474518	24.327917439914852	24.602839338206376	25.587935677404257
32	25.384171661416193	24.4095642817466	24.46967607608852	25.736587980748688
33	25.02070880937699	24.29228311588679	24.90194269510709	25.785065379629135
34	25.656319251707682	24.441762045063015	24.76387656128916	25.138042141940147
35	25.837793114699654	24.215471775434835	25.177153100871173	24.769582008994337
36	24.97552827843381	24.744892013673088	24.73391858297079	25.545661124922315
37	25.794685490707913	24.340153455169087	24.737338394770756	25.12782265935225
38	25.17615755127408	24.19505515848353	24.964360627719078	25.664426662523304
39	25.837608655329902	24.301308962298506	24.389388849882543	25.47169353248905
40	25.763187538844	24.4944453076445	24.701775170913614	25.04059198259789
41	25.096673949987863	24.805341102209276	24.998203447438698	25.099781500364166
42	25.94177001291626	24.65033844480485	24.59682823319187	24.811063309087025
43	25.567413182456928	24.089919851765096	24.768455799020508	25.574211166757465
44	25.09354493798794	24.51377511728928	24.747431095279392	25.64524884944339
45	25.265842256245712	24.599949890555536	24.738430642119514	25.395777211079235
46	25.37315172149917	24.484191185753325	24.573145506350134	25.56951158639737
47	25.36535531405165	24.465331910335607	25.00284049250741	25.166472283105335
48	25.820968429557677	24.96705443859501	24.129649645683475	25.082327486163834
49	25.37985980682165	24.871761504561427	24.178370472807224	25.570008215809704
50	25.342510983269694	25.088759580013754	24.433356510524906	25.13537292619165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	23.5
2	33.0
3	94.5
4	156.0
5	165.0
6	174.0
7	142.5
8	111.0
9	147.0
10	183.0
11	326.5
12	470.0
13	907.0
14	1344.0
15	1597.0
16	1850.0
17	1847.0
18	1844.0
19	1763.0
20	1682.0
21	1965.5
22	2249.0
23	2738.0
24	3227.0
25	4153.5
26	5080.0
27	6300.0
28	7520.0
29	8930.0
30	10340.0
31	11954.0
32	13568.0
33	15660.5
34	17753.0
35	20402.0
36	23051.0
37	27164.5
38	31278.0
39	35327.5
40	39377.0
41	43498.5
42	47620.0
43	50920.0
44	54220.0
45	59172.0
46	64124.0
47	68565.5
48	73007.0
49	77097.5
50	81188.0
51	81249.0
52	81310.0
53	81996.0
54	82682.0
55	82579.0
56	82476.0
57	79327.5
58	76179.0
59	70761.0
60	65343.0
61	57604.0
62	49865.0
63	42864.0
64	35863.0
65	30694.5
66	25526.0
67	21588.5
68	17651.0
69	15207.5
70	12764.0
71	9837.0
72	6910.0
73	5876.5
74	4843.0
75	3645.0
76	2447.0
77	2086.0
78	1725.0
79	1431.5
80	1138.0
81	914.5
82	691.0
83	570.0
84	449.0
85	354.0
86	259.0
87	179.5
88	100.0
89	63.5
90	27.0
91	20.0
92	13.0
93	13.0
94	13.0
95	12.5
96	12.0
97	10.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031075201988812924
2	0.005341050341827222
3	9.71100062150404E-5
4	9.71100062150404E-5
5	0.0
6	3.884400248601616E-4
7	0.0
8	0.0
9	0.0
10	9.71100062150404E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.768800497203232E-4
17	0.0
18	9.71100062150404E-5
19	9.71100062150404E-5
20	3.884400248601616E-4
21	0.0
22	0.0
23	0.0
24	9.711000621504039E-4
25	9.71100062150404E-5
26	5.826600372902424E-4
27	3.884400248601616E-4
28	0.00242775015537601
29	9.711000621504039E-4
30	0.0015537600994406464
31	0.0012624300807955252
32	0.0011653200745804849
33	4.8555003107520194E-4
34	1.942200124300808E-4
35	9.71100062150404E-5
36	0.0
37	0.0012624300807955252
38	0.0
39	0.0012624300807955252
40	0.0
41	9.711000621504039E-4
42	0.00485550031075202
43	0.004175730267246737
44	0.003981510254816656
45	0.0013595400870105656
46	0.001747980111870727
47	8.739900559353635E-4
48	0.003010410192666252
49	0.003690180236171535
50	0.0011653200745804849
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1029760.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.84695458721099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00131455016822	46.681411296266766
2	13.278518953110266	15.89357841544421
3	3.9089898898619873	7.018234212613117
4	1.60137326360161	3.8334925193575757
5	0.862694767404492	2.5814827283740587
6	0.5261469389250859	1.8892975176029811
7	0.35792299931497407	1.499442104000512
8	0.24923474783756133	1.193275250831161
9	0.1883837250574481	1.0146773014634463
>10	0.9213754505704823	9.660858294697375
>50	0.0583204534646896	2.4311139356457527
>100	0.042294941962664756	4.881644696170241
>500	0.0034293187205503496	1.4214917275327799
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.71100062150404E-5	0.0
5	0.0	0.0	0.0	9.71100062150404E-5	0.0
6	0.0	0.0	0.0	9.71100062150404E-5	0.0
7	0.0	0.0	0.0	9.71100062150404E-5	9.71100062150404E-5
8	0.0	0.0	0.0	9.71100062150404E-5	9.71100062150404E-5
9	0.0	0.0	0.0	9.71100062150404E-5	9.71100062150404E-5
10	0.0	0.0	0.0	9.71100062150404E-5	1.942200124300808E-4
11	0.0	0.0	0.0	9.71100062150404E-5	1.942200124300808E-4
12	0.0	0.0	0.0	9.71100062150404E-5	7.768800497203232E-4
13	0.0	0.0	0.0	9.71100062150404E-5	0.0011653200745804849
14	0.0	0.0	0.0	9.71100062150404E-5	0.0011653200745804849
15	0.0	0.0	0.0	9.71100062150404E-5	0.0018450901180857675
16	0.0	0.0	0.0	1.942200124300808E-4	0.001942200124300808
17	0.0	0.0	0.0	1.942200124300808E-4	0.0020393101305158485
18	0.0	0.0	0.0	1.942200124300808E-4	0.0021364201367308887
19	0.0	0.0	0.0	1.942200124300808E-4	0.0021364201367308887
20	0.0	0.0	0.0	2.913300186451212E-4	0.0025248601615910503
21	0.0	0.0	0.0	7.768800497203232E-4	0.0027190801740211313
22	0.0	0.0	0.0	0.0010682100683654444	0.0027190801740211313
23	0.0	0.0	0.0	0.0015537600994406464	0.0027190801740211313
24	0.0	0.0	0.0	0.0023306401491609697	0.003010410192666252
25	0.0	0.0	0.0	0.002913300186451212	0.003010410192666252
26	0.0	0.0	0.0	0.003884400248601616	0.003398850217526414
27	0.0	0.0	0.0	0.004467060285891858	0.0034959602237414544
28	0.0	0.0	0.0	0.00495261031696706	0.0034959602237414544
29	0.0	0.0	0.0	0.006020820385332504	0.003690180236171535
30	0.0	0.0	0.0	0.009128340584213798	0.003690180236171535
31	0.0	0.0	0.0	0.014178060907395897	0.003884400248601616
32	0.0	0.0	0.0	0.018742231199502798	0.004175730267246737
33	0.0	0.0	0.0	0.024860161591050343	0.004175730267246737
34	0.0	0.0	0.0	0.030686761963952765	0.004175730267246737
35	0.0	0.0	0.0	0.037775792417650715	0.004175730267246737
36	0.0	0.0	0.0	0.045544592914853944	0.004175730267246737
37	0.0	0.0	0.0	0.057974673710379114	0.004175730267246737
38	0.0	0.0	0.0	0.07351227470478558	0.004369950279676818
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	575	0.0	27.172506	1
GTATCAA	1315	0.0	25.10179	1
TATACCG	55	1.5936454E-4	23.999443	5
GTCGTAA	70	3.2153308E-5	21.99949	30
GTCCTAT	230	0.0	18.178787	1
AACCGCG	140	8.366078E-8	17.286154	41
ACGGTAT	255	0.0	17.254501	9
TCCTATT	245	0.0	17.060827	2
TACGACG	285	0.0	16.982063	5
GTAATAC	105	3.774219E-5	16.761517	3
GTGTATA	175	3.610694E-9	16.347242	1
GCGCAAT	150	1.8362698E-7	16.133743	35
CGCAATA	150	1.8362698E-7	16.133743	36
CTAGCGG	150	1.8372884E-7	16.13296	29
GACGGTA	280	0.0	15.7139225	8
CTATTCC	240	3.6379788E-12	15.582972	4
ATCAACG	2105	0.0	15.572084	3
CTACGAC	325	0.0	15.5688715	4
CCTTATA	85	0.002979999	15.529053	2
GCACCGT	85	0.002979999	15.529053	6
>>END_MODULE