FastQCFastQC Report
Thu 2 Feb 2017
SRR4062050_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062050_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150070
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19980.17372855565313414No Hit
TATCAACGCAGAGTACTTTTTTTTT14580.1267748919630979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTG350.002170134616.2852675
CGAACGA456.7596056E-414.77801416
GGTATCA5000.014.0528821
GTCCTAA1051.378794E-913.5645591
CGAACGT500.001500426613.2984754
TGCATCG604.0915294E-412.6663185
CGAGCCG1457.2759576E-1212.449018515
ACTGCGC1001.438384E-712.3518128
CGATAAC550.003062238712.09373710
CGTTATT1103.831883E-812.085842
GTATCAA12550.012.0297631
CGTCGTA1209.964424E-911.87777710
TTATGCT1052.7213173E-711.7605574
CCGTCGT1302.6029738E-911.6945329
GAATTTC1900.011.49868318
AACCGCG752.0700932E-411.4016727
AGACGAT752.0700932E-411.4016727
GTCCTAC1501.7826096E-1011.3942291
GTCCATA752.0826887E-411.3942291
GTCTTAG1104.9958544E-711.221591
Produced by FastQC (version 0.11.5)