##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062050_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150070
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263823941151408	32.0	32.0	32.0	32.0	32.0
2	30.81221143060857	32.0	32.0	32.0	32.0	32.0
3	30.847311902753745	32.0	32.0	32.0	32.0	32.0
4	30.847079742972166	32.0	32.0	32.0	32.0	32.0
5	30.76523776813585	32.0	32.0	32.0	32.0	32.0
6	34.424301999008755	36.0	36.0	36.0	32.0	36.0
7	34.3669733146678	36.0	36.0	36.0	32.0	36.0
8	34.3285608702079	36.0	36.0	36.0	32.0	36.0
9	34.45100037389029	36.0	36.0	36.0	32.0	36.0
10	34.14576764892572	36.0	36.0	36.0	32.0	36.0
11	34.428432182388896	36.0	36.0	36.0	32.0	36.0
12	34.25994417731095	36.0	36.0	36.0	32.0	36.0
13	34.34628240020173	36.0	36.0	36.0	32.0	36.0
14	34.246916274661544	36.0	36.0	36.0	32.0	36.0
15	34.21017851087325	36.0	36.0	36.0	32.0	36.0
16	34.201037328162634	36.0	36.0	36.0	32.0	36.0
17	34.138828071334785	36.0	36.0	36.0	32.0	36.0
18	34.13976192753485	36.0	36.0	36.0	32.0	36.0
19	34.15123514220873	36.0	36.0	36.0	32.0	36.0
20	34.13755771387829	36.0	36.0	36.0	32.0	36.0
21	34.1163981322876	36.0	36.0	36.0	32.0	36.0
22	34.08987365986418	36.0	36.0	36.0	32.0	36.0
23	34.05344892050049	36.0	36.0	36.0	32.0	36.0
24	34.02954515812081	36.0	36.0	36.0	32.0	36.0
25	33.67837783787074	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	15.0
5	79.0
6	247.0
7	48.0
8	210.0
9	165.0
10	83.0
11	27.0
12	36.0
13	64.0
14	172.0
15	253.0
16	449.0
17	558.0
18	746.0
19	1000.0
20	1466.0
21	2123.0
22	3209.0
23	4701.0
24	6612.0
25	9398.0
26	12859.0
27	16946.0
28	22421.0
29	29673.0
30	39203.0
31	53324.0
32	75589.0
33	106741.0
34	234742.0
35	526910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.193801752419645	17.490980960239707	11.603584834622678	25.71163245271797
2	16.939838444616413	18.98493135200466	37.57597363638735	26.499256566991576
3	19.74430965955778	22.269127865169125	27.807172302734717	30.179390172538373
4	13.38238465605521	15.165154719501336	34.36630398486198	37.08615663958148
5	15.343058887383162	35.90997889858622	32.96482691795847	15.782135296072145
6	35.467429539935566	33.85780870569954	16.291712018821254	14.383049735543645
7	31.453376228178293	29.66403100127431	19.541700613479847	19.34089215706755
8	28.395173490758356	33.35181603416369	18.624121056177543	19.628889418900407
9	26.78478002171099	15.12813749480947	18.70409592531559	39.38298655816395
10	17.31781217894022	26.459505110836368	30.206958399359184	26.01572431086423
11	37.16685474657973	21.449904566858926	21.429355094875866	19.953885591685474
12	24.419145264616922	23.5866288920271	28.069262018900087	23.924963824455887
13	29.596842019115744	19.39896324337313	24.965479173450394	26.038715564060727
14	23.445548381368084	19.523955856838544	24.684844047750666	32.34565171404271
15	25.245510546135257	27.105026011623607	21.782285975490304	25.867177466750835
16	25.884899126113687	25.978050984536793	23.2761247433971	24.86092514595242
17	24.29163684863934	26.28635405185638	24.637264785823856	24.784744313680424
18	24.921329242018672	25.273182302887857	25.576287784814262	24.229200670279212
19	25.541396450775377	25.18880659954897	25.042496172515747	24.227300777159908
20	25.686872484716826	24.598512066396513	24.848125635348346	24.866489813538315
21	26.337162396792397	24.313087947196696	24.50699248663425	24.842757169376657
22	25.93659340343694	24.286886865453127	24.878424122464576	24.898095608645356
23	24.665240051622696	24.47787716896686	25.349336484765995	25.507546294644452
24	24.705979625495548	24.93731423821895	25.247849188631704	25.108856947653802
25	24.933356193413687	24.63840893840746	25.081917705451346	25.346317162727512
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	558.0
1	558.0
2	478.5
3	399.0
4	399.0
5	399.0
6	830.0
7	1261.0
8	1261.0
9	1261.0
10	1503.0
11	1745.0
12	1745.0
13	1745.0
14	2341.0
15	2937.0
16	2937.0
17	2937.0
18	4900.5
19	6864.0
20	6864.0
21	6864.0
22	10725.0
23	14586.0
24	14586.0
25	14586.0
26	21440.0
27	28294.0
28	28294.0
29	28294.0
30	36204.0
31	44114.0
32	44114.0
33	44114.0
34	55319.0
35	66524.0
36	66524.0
37	66524.0
38	78978.5
39	91433.0
40	91433.0
41	91433.0
42	105750.0
43	120067.0
44	120067.0
45	120067.0
46	133748.5
47	147430.0
48	147430.0
49	147430.0
50	152895.5
51	158361.0
52	158361.0
53	158361.0
54	149758.0
55	141155.0
56	141155.0
57	141155.0
58	129783.0
59	118411.0
60	118411.0
61	118411.0
62	104065.0
63	89719.0
64	89719.0
65	89719.0
66	72917.0
67	56115.0
68	56115.0
69	56115.0
70	43082.0
71	30049.0
72	30049.0
73	30049.0
74	22526.5
75	15004.0
76	15004.0
77	15004.0
78	11898.5
79	8793.0
80	8793.0
81	8793.0
82	6137.0
83	3481.0
84	3481.0
85	3481.0
86	2550.5
87	1620.0
88	1620.0
89	1620.0
90	1085.0
91	550.0
92	550.0
93	550.0
94	338.5
95	127.0
96	127.0
97	127.0
98	300.0
99	473.0
100	473.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.608536871668681E-4
2	0.0
3	6.08658603389359E-4
4	0.01026024502856348
5	0.033389271957359114
6	0.0635613484396602
7	0.10555879207352595
8	0.10851513386141713
9	0.11677550062170129
10	0.13303538045510274
11	0.14103489352822002
12	0.13077464849965656
13	0.12938342883476658
14	0.11764501291225751
15	0.13433964889093705
16	0.12242733051031676
17	0.1248619649238742
18	0.1126018416270314
19	0.09938525481057675
20	0.09512464458685123
21	0.09138574173745945
22	0.10425452363769162
23	0.08373403358056467
24	0.09434208352535063
25	0.09138574173745945
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1150070.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.4474288830972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4968625401637	49.4632523624916
2	11.982899403293166	14.726367177943292
3	3.383592335880079	6.237391482851518
4	1.3936787608298515	3.425519061679013
5	0.7326271198952847	2.2509026423796916
6	0.45390705412108584	1.6734852856584943
7	0.30196639973977757	1.2988541211166418
8	0.209683141217374	1.0307591926351212
9	0.15324334507134973	0.8474768593271718
>10	0.7789185266782788	8.488849777399077
>50	0.0589676885421319	2.5542086617094344
>100	0.049811117040427066	6.112257209182806
>500	0.00341561558000214	1.493651660911083
>1k	4.269519475002675E-4	0.39702450471524103
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1998	0.17372855565313414	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1458	0.1267748919630979	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.69512290556227E-5
7	0.0	0.0	0.0	8.69512290556227E-5	8.69512290556227E-5
8	0.0	0.0	0.0	8.69512290556227E-5	8.69512290556227E-5
9	0.0	0.0	0.0	8.69512290556227E-5	1.739024581112454E-4
10	0.0	0.0	0.0	8.69512290556227E-5	1.739024581112454E-4
11	0.0	0.0	0.0	8.69512290556227E-5	1.739024581112454E-4
12	0.0	0.0	0.0	8.69512290556227E-5	2.6085368716686813E-4
13	0.0	0.0	0.0	8.69512290556227E-5	2.6085368716686813E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACTG	35	0.0021701346	16.285267	5
CGAACGA	45	6.7596056E-4	14.778014	16
GGTATCA	500	0.0	14.052882	1
GTCCTAA	105	1.378794E-9	13.564559	1
CGAACGT	50	0.0015004266	13.298475	4
TGCATCG	60	4.0915294E-4	12.666318	5
CGAGCCG	145	7.2759576E-12	12.4490185	15
ACTGCGC	100	1.438384E-7	12.351812	8
CGATAAC	55	0.0030622387	12.093737	10
CGTTATT	110	3.831883E-8	12.08584	2
GTATCAA	1255	0.0	12.029763	1
CGTCGTA	120	9.964424E-9	11.877777	10
TTATGCT	105	2.7213173E-7	11.760557	4
CCGTCGT	130	2.6029738E-9	11.694532	9
GAATTTC	190	0.0	11.498683	18
AACCGCG	75	2.0700932E-4	11.401672	7
AGACGAT	75	2.0700932E-4	11.401672	7
GTCCTAC	150	1.7826096E-10	11.394229	1
GTCCATA	75	2.0826887E-4	11.394229	1
GTCTTAG	110	4.9958544E-7	11.22159	1
>>END_MODULE