FastQCFastQC Report
Thu 2 Feb 2017
SRR4062050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150070
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA300.005745315529.33289710
GGTATCA6950.026.5987831
GTATCAA14650.025.3872931
CGGATCG701.1323355E-625.14248526
GCGAATT500.002579932822.00063140
CGCAATA1900.021.99967436
TCTAGCG1950.021.43558128
ATAAGCG550.004483866519.9997043
TAGATCG550.004483866519.9997045
CTAGCGG2150.019.44157229
CGACGGT2950.018.6437937
ACTGCGT951.5972042E-518.5260418
CAATACG2300.018.17443538
GCTCGAC851.4309186E-418.11737811
TCAACGC21500.017.599744
ACGACGG3150.017.460066
CAACGCA22000.017.1997435
TAACACT1802.8558134E-1017.1108594
AACGCAG22400.017.0890336
GCGCAAT2450.017.06097235