##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150070
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28768683645343	32.0	32.0	32.0	32.0	32.0
2	31.46390567530672	32.0	32.0	32.0	32.0	32.0
3	31.584499204396256	32.0	32.0	32.0	32.0	32.0
4	31.66284139226308	32.0	32.0	32.0	32.0	32.0
5	31.63487352943734	32.0	32.0	32.0	32.0	32.0
6	35.291472692966515	36.0	36.0	36.0	36.0	36.0
7	35.29310041997444	36.0	36.0	36.0	36.0	36.0
8	35.245735476970964	36.0	36.0	36.0	36.0	36.0
9	35.33273279017799	36.0	36.0	36.0	36.0	36.0
10	35.214252175954506	36.0	36.0	36.0	36.0	36.0
11	35.33076856191362	36.0	36.0	36.0	36.0	36.0
12	35.25376890102341	36.0	36.0	36.0	36.0	36.0
13	35.28362882259341	36.0	36.0	36.0	36.0	36.0
14	35.2546079803838	36.0	36.0	36.0	36.0	36.0
15	35.22759745058996	36.0	36.0	36.0	36.0	36.0
16	35.24183397532324	36.0	36.0	36.0	36.0	36.0
17	35.230696392393504	36.0	36.0	36.0	36.0	36.0
18	35.22333944890311	36.0	36.0	36.0	36.0	36.0
19	35.21046023285539	36.0	36.0	36.0	36.0	36.0
20	35.21102889389341	36.0	36.0	36.0	36.0	36.0
21	35.20317545888511	36.0	36.0	36.0	36.0	36.0
22	35.185551314267826	36.0	36.0	36.0	36.0	36.0
23	35.15436364743016	36.0	36.0	36.0	36.0	36.0
24	35.1423626387959	36.0	36.0	36.0	36.0	36.0
25	35.13020163990018	36.0	36.0	36.0	36.0	36.0
26	35.08503308494266	36.0	36.0	36.0	36.0	36.0
27	35.071526081021155	36.0	36.0	36.0	36.0	36.0
28	35.04738668081073	36.0	36.0	36.0	36.0	36.0
29	35.033621431738936	36.0	36.0	36.0	36.0	36.0
30	35.01422348204892	36.0	36.0	36.0	36.0	36.0
31	35.01514951263836	36.0	36.0	36.0	36.0	36.0
32	34.98653734120532	36.0	36.0	36.0	36.0	36.0
33	34.970287895519405	36.0	36.0	36.0	36.0	36.0
34	34.95929378211761	36.0	36.0	36.0	36.0	36.0
35	34.94329736450825	36.0	36.0	36.0	36.0	36.0
36	34.924237655099255	36.0	36.0	36.0	36.0	36.0
37	34.91319137096003	36.0	36.0	36.0	36.0	36.0
38	34.89170137469893	36.0	36.0	36.0	32.0	36.0
39	34.87488500699958	36.0	36.0	36.0	32.0	36.0
40	34.86992791743111	36.0	36.0	36.0	32.0	36.0
41	34.84437642926083	36.0	36.0	36.0	32.0	36.0
42	34.80850556922622	36.0	36.0	36.0	32.0	36.0
43	34.809876790108426	36.0	36.0	36.0	32.0	36.0
44	34.75954246263271	36.0	36.0	36.0	32.0	36.0
45	34.75242550453451	36.0	36.0	36.0	32.0	36.0
46	34.719451859452036	36.0	36.0	36.0	32.0	36.0
47	34.702217256340916	36.0	36.0	36.0	32.0	36.0
48	34.674345909379426	36.0	36.0	36.0	32.0	36.0
49	34.65780778561305	36.0	36.0	36.0	32.0	36.0
50	34.255319241437476	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	11.0
21	36.0
22	74.0
23	220.0
24	506.0
25	1255.0
26	2591.0
27	4858.0
28	8311.0
29	13520.0
30	20387.0
31	30241.0
32	46610.0
33	79231.0
34	184384.0
35	757832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91488554830438	17.985664073814103	12.036280800452987	26.063169577428525
2	16.350739954591422	19.54020989510462	37.30938093204974	26.799669218254213
3	19.116712330672907	22.801454176148	28.061060790371346	30.02077270280775
4	13.07198692253515	15.561835366542907	34.52015964245654	36.8460180684654
5	14.952742007008268	36.21536080412497	32.931386785152206	15.900510403714557
6	35.67623827460637	34.03259633343419	16.11930772789087	14.17185766406856
7	31.37417722399506	29.774796316745938	19.602458980757692	19.248567478501307
8	28.273148590955334	33.565087342509585	18.551305572704273	19.61045849383081
9	26.841061848409225	14.8635300459972	18.65982070656569	39.635587399027884
10	17.217662592418367	26.49875790061292	30.26714049330953	26.01643901365918
11	37.379116053805426	21.254271479127357	21.46356308746424	19.90304937960298
12	24.465119514464337	23.460832818871893	28.15211248010991	23.92193518655386
13	29.690627527020098	19.27317467632405	25.024737624666326	26.01146017198953
14	23.541697461893623	19.24830662481414	24.798055770518314	32.41194014277392
15	25.258114723451612	27.05991809194223	21.842148738772423	25.839818445833735
16	25.86945539315809	25.85249974131762	23.362888338772184	24.915156526752106
17	24.36790804038015	26.23153373272931	24.667802829392993	24.732755397497545
18	25.002238996095016	25.121275332175724	25.67419867851407	24.20228699321519
19	25.68808855113167	24.982740181032458	25.121514342605234	24.20765692523064
20	25.797433881940485	24.452465254107597	25.003825874038316	24.746274989913605
21	26.39352387245994	24.225829732103264	24.732929299955657	24.647717095481145
22	25.92111784500074	24.217221560426758	25.00952115958159	24.852139434990914
23	24.63773912695673	24.303063555317117	25.580552123394334	25.47864519433182
24	24.78998940056223	24.745643926713452	25.35239435125615	25.11197232146817
25	25.05086655745573	24.37568908968861	25.28250503448492	25.290939318370743
26	24.70173321241221	25.151535440294037	25.640118219816166	24.506613127477586
27	25.266897725869647	24.726667860801033	25.016651218277907	24.989783195051416
28	24.9448069874919	24.62143114894505	25.421392889905263	25.012368973657782
29	24.658975500493884	24.871746268033778	25.462582951905283	25.006695279567047
30	24.679102024774487	24.961610498289648	25.536453070899277	24.822834406036588
31	25.349066923115714	24.685278931817827	24.769621917350833	25.196032227715627
32	25.06530071369941	24.783055551691035	24.859573032457323	25.29207070215223
33	24.798729117520978	24.518484575246486	25.27722297918295	25.405563328049585
34	25.254986252915064	24.65484469532947	25.320634887510412	24.76953416424506
35	25.52209865486449	24.50207378681298	25.468362795308114	24.507464763014426
36	24.64984679166797	24.9498290535864	25.25128948896935	25.14903466577628
37	25.527996487124526	24.670733138849883	25.038019920786397	24.763250453239195
38	24.8202283339275	24.54511464519551	25.478362186649505	25.156294834227484
39	25.45382138605838	24.5878718370321	24.82542321447359	25.132883562435925
40	25.56950930770241	24.648347186125356	25.058496490210587	24.72364701596165
41	24.759926404095772	25.051127589421114	25.428063133877764	24.76088287260535
42	25.556380660543887	24.997869605972756	25.093259085478365	24.352490648004995
43	25.27362543814417	24.339381628683302	25.17623591221378	25.210757020958745
44	24.744700980153457	24.877741681912724	25.141736172462487	25.23582116547133
45	24.945111034399403	25.037541878650387	25.017629683604785	24.99971740334542
46	24.842789717334522	24.85305012295096	25.1130383669608	25.19112179275372
47	24.95485006395335	24.861376436124473	25.310658515186642	24.87311498473554
48	25.581874607076493	25.167016946470234	24.454602607055623	24.796505839397653
49	25.03932502689058	25.253406212528184	24.589684973709407	25.117583786871833
50	24.987239740708052	25.406002321628097	24.743860076257224	24.862897861406626
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	317.0
1	192.0
2	67.0
3	151.0
4	235.0
5	255.5
6	276.0
7	211.5
8	147.0
9	211.5
10	276.0
11	470.0
12	664.0
13	1161.0
14	1658.0
15	2035.0
16	2412.0
17	2360.0
18	2308.0
19	2170.5
20	2033.0
21	2453.5
22	2874.0
23	3412.5
24	3951.0
25	5149.0
26	6347.0
27	7999.0
28	9651.0
29	11215.5
30	12780.0
31	14930.5
32	17081.0
33	19945.5
34	22810.0
35	26680.0
36	30550.0
37	35168.0
38	39786.0
39	44342.5
40	48899.0
41	52940.5
42	56982.0
43	60464.5
44	63947.0
45	69682.0
46	75417.0
47	79917.5
48	84418.0
49	87792.0
50	91166.0
51	89633.0
52	88100.0
53	87104.0
54	86108.0
55	86229.0
56	86350.0
57	82674.0
58	78998.0
59	72249.0
60	65500.0
61	57973.0
62	50446.0
63	43497.0
64	36548.0
65	30969.0
66	25390.0
67	21987.0
68	18584.0
69	16208.5
70	13833.0
71	10875.0
72	7917.0
73	7017.5
74	6118.0
75	4597.5
76	3077.0
77	2660.0
78	2243.0
79	1989.0
80	1735.0
81	1336.5
82	938.0
83	774.5
84	611.0
85	472.0
86	333.0
87	220.0
88	107.0
89	69.5
90	32.0
91	26.0
92	20.0
93	15.0
94	10.0
95	9.0
96	8.0
97	7.5
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03225890597963602
2	0.00582573234672672
3	2.608536871668681E-4
4	0.0
5	0.0
6	1.7390245811124542E-4
7	0.0
8	0.0
9	0.0
10	8.695122905562271E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.564635196118497E-4
17	0.0
18	8.695122905562271E-5
19	0.0
20	5.217073743337362E-4
21	0.0
22	0.0
23	8.695122905562271E-5
24	7.825610615006043E-4
25	1.7390245811124542E-4
26	7.825610615006043E-4
27	3.4780491622249084E-4
28	0.0013042684358343405
29	5.217073743337362E-4
30	0.0013912196648899634
31	3.4780491622249084E-4
32	5.217073743337362E-4
33	2.608536871668681E-4
34	6.956098324449817E-4
35	0.0
36	1.7390245811124542E-4
37	0.0013042684358343405
38	0.0
39	2.608536871668681E-4
40	8.695122905562271E-5
41	5.217073743337362E-4
42	0.004173658994669889
43	0.004260610223725513
44	0.003999756536558644
45	0.0018259758101680768
46	0.0015651221230012086
47	0.0011303659777230951
48	0.00321719547505804
49	0.0033910979331692854
50	0.0013042684358343405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1150070.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.20141810378826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.65574481594294	50.975574505848606
2	12.134262405214697	15.33805183306107
3	3.266568779103643	6.193553375187314
4	1.3432897430137163	3.3959126673096063
5	0.6957746889744486	2.198697351195368
6	0.4498821309362104	1.7059913192833585
7	0.28207691008209346	1.2479362512066152
8	0.20783421902662347	1.0508333898380748
9	0.15567107171206168	0.8854769240944934
>10	0.7126536685283625	7.99363969676679
>50	0.0522282257267696	2.3372815231827206
>100	0.04069158419824368	5.16023620089873
>500	0.003044944411966777	1.330143501390269
>1k	2.768131283606161E-4	0.18667146073700075
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.69512290556227E-5
7	0.0	0.0	0.0	0.0	8.69512290556227E-5
8	0.0	0.0	0.0	0.0	8.69512290556227E-5
9	0.0	0.0	0.0	0.0	8.69512290556227E-5
10	0.0	0.0	0.0	0.0	8.69512290556227E-5
11	0.0	0.0	0.0	0.0	8.69512290556227E-5
12	0.0	0.0	0.0	0.0	8.69512290556227E-5
13	0.0	0.0	0.0	0.0	1.739024581112454E-4
14	0.0	0.0	0.0	0.0	1.739024581112454E-4
15	0.0	0.0	0.0	0.0	1.739024581112454E-4
16	0.0	0.0	0.0	8.69512290556227E-5	3.478049162224908E-4
17	0.0	0.0	0.0	8.69512290556227E-5	3.478049162224908E-4
18	0.0	0.0	0.0	8.69512290556227E-5	3.478049162224908E-4
19	0.0	8.69512290556227E-5	0.0	8.69512290556227E-5	5.217073743337363E-4
20	0.0	2.6085368716686813E-4	0.0	2.6085368716686813E-4	5.217073743337363E-4
21	0.0	3.478049162224908E-4	0.0	4.347561452781135E-4	5.217073743337363E-4
22	0.0	3.478049162224908E-4	0.0	4.347561452781135E-4	5.217073743337363E-4
23	0.0	3.478049162224908E-4	0.0	6.956098324449816E-4	6.086586033893589E-4
24	0.0	3.478049162224908E-4	0.0	0.0013042684358343405	6.086586033893589E-4
25	0.0	3.478049162224908E-4	0.0	0.001478170893945586	6.086586033893589E-4
26	0.0	3.478049162224908E-4	0.0	0.001999878268279322	6.086586033893589E-4
27	0.0	3.478049162224908E-4	0.0	0.002347683184501813	6.086586033893589E-4
28	0.0	3.478049162224908E-4	0.0	0.0033910979331692854	6.086586033893589E-4
29	0.0	3.478049162224908E-4	0.0	0.00547792743050423	6.086586033893589E-4
30	0.0	3.478049162224908E-4	0.0	0.009390732738007251	6.086586033893589E-4
31	0.0	3.478049162224908E-4	0.0	0.017477197040180165	6.086586033893589E-4
32	0.0	3.478049162224908E-4	0.0	0.025911466258575565	6.956098324449816E-4
33	8.69512290556227E-5	3.478049162224908E-4	0.0	0.03382402810263723	6.956098324449816E-4
34	8.69512290556227E-5	3.478049162224908E-4	0.0	0.043301712069700106	6.956098324449816E-4
35	8.69512290556227E-5	3.478049162224908E-4	0.0	0.05434451815976419	6.956098324449816E-4
36	8.69512290556227E-5	3.478049162224908E-4	0.0	0.06999573938977627	6.956098324449816E-4
37	8.69512290556227E-5	3.478049162224908E-4	0.0	0.0907770831340701	6.956098324449816E-4
38	8.69512290556227E-5	3.478049162224908E-4	0.0	0.11625379324736755	6.956098324449816E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	30	0.0057453155	29.332897	10
GGTATCA	695	0.0	26.598783	1
GTATCAA	1465	0.0	25.387293	1
CGGATCG	70	1.1323355E-6	25.142485	26
GCGAATT	50	0.0025799328	22.000631	40
CGCAATA	190	0.0	21.999674	36
TCTAGCG	195	0.0	21.435581	28
ATAAGCG	55	0.0044838665	19.999704	3
TAGATCG	55	0.0044838665	19.999704	5
CTAGCGG	215	0.0	19.441572	29
CGACGGT	295	0.0	18.643793	7
ACTGCGT	95	1.5972042E-5	18.526041	8
CAATACG	230	0.0	18.174435	38
GCTCGAC	85	1.4309186E-4	18.117378	11
TCAACGC	2150	0.0	17.59974	4
ACGACGG	315	0.0	17.46006	6
CAACGCA	2200	0.0	17.199743	5
TAACACT	180	2.8558134E-10	17.110859	4
AACGCAG	2240	0.0	17.089033	6
GCGCAAT	245	0.0	17.060972	35
>>END_MODULE