FastQCFastQC Report
Thu 2 Feb 2017
SRR4062049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087095
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC19190.1765255106499432No Hit
GTATCAACGCAGAGTACTTTTTTTT14540.13375096012767973No Hit
GGGTAGGCACACGCTGAGCCAGTCA13530.12446014377768273No Hit
TCGTAGTTCCGACCATAAACGATGC12810.11783698756778387No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC12490.11489336258560659No Hit
ATCAGATACCGTCGTAGTTCCGACC12380.11388149149798316No Hit
GAATAGGACCGCGGTTCTATTTTGT12380.11388149149798316No Hit
GTATCAACGCAGAGTACATGGGGAA11610.10679839388461909No Hit
GGTATCAACGCAGAGTACATGGGGA11120.10229096813066016No Hit
CTGTAGGACGTGGAATATGGCAAGA10980.1010031322009576No Hit
GATTAAGAGGGACGGCCGGGGGCAT10880.1000832493940272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAC307.7475375E-418.9901921
GCTCGTA402.7574488E-416.6271369
ATTGGAC402.7622987E-416.6233083
GGTATCA6800.016.337151
CTCGTAG456.748702E-414.7810410
CCGTCCT400.00527324614.2518329
AGGTCGT400.00527324614.2518326
CGGTCCA1301.8189894E-1213.8876810
CGCCGGT1400.013.5737977
GCCGGTC1400.013.5731738
CCGACCA2600.013.1555389
CGACCAT2550.013.04209310
CGGACCA2700.013.0201919
GTATCAA21700.012.9518371
AGCCCCG2350.012.93544916
CCGGTCC1403.6379788E-1212.8945149
AGGCCCG1950.012.66946210
CGTCGTA1950.012.66946210
GGTCGTG604.0842398E-412.6688787
ACGATCA751.4756592E-512.6682959
Produced by FastQC (version 0.11.5)