##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062049_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1087095
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32230301859543	32.0	32.0	32.0	32.0	32.0
2	30.98668285660407	32.0	32.0	32.0	32.0	32.0
3	31.005004162469703	32.0	32.0	32.0	32.0	32.0
4	31.005782383324366	32.0	32.0	32.0	32.0	32.0
5	31.003489115486687	32.0	32.0	32.0	32.0	32.0
6	34.649385748255675	36.0	36.0	36.0	32.0	36.0
7	34.56852528987807	36.0	36.0	36.0	32.0	36.0
8	34.5449026993961	36.0	36.0	36.0	32.0	36.0
9	34.620370804759474	36.0	36.0	36.0	32.0	36.0
10	34.416114507011805	36.0	36.0	36.0	32.0	36.0
11	34.602717333811675	36.0	36.0	36.0	32.0	36.0
12	34.472261393898414	36.0	36.0	36.0	32.0	36.0
13	34.53435256348341	36.0	36.0	36.0	32.0	36.0
14	34.472476646475236	36.0	36.0	36.0	32.0	36.0
15	34.443834255515846	36.0	36.0	36.0	32.0	36.0
16	34.448534856659265	36.0	36.0	36.0	32.0	36.0
17	34.394511979173856	36.0	36.0	36.0	32.0	36.0
18	34.3866607794167	36.0	36.0	36.0	32.0	36.0
19	34.38617784094306	36.0	36.0	36.0	32.0	36.0
20	34.364813562752104	36.0	36.0	36.0	32.0	36.0
21	34.33864473666055	36.0	36.0	36.0	32.0	36.0
22	34.31086703553967	36.0	36.0	36.0	32.0	36.0
23	34.289265427584525	36.0	36.0	36.0	32.0	36.0
24	34.26918254614362	36.0	36.0	36.0	32.0	36.0
25	33.93064635565429	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	8.0
5	93.0
6	216.0
7	38.0
8	205.0
9	156.0
10	87.0
11	28.0
12	55.0
13	55.0
14	121.0
15	160.0
16	333.0
17	460.0
18	602.0
19	821.0
20	1173.0
21	1622.0
22	2573.0
23	3573.0
24	5071.0
25	7359.0
26	10141.0
27	13524.0
28	17962.0
29	24466.0
30	32042.0
31	43903.0
32	62764.0
33	91927.0
34	216779.0
35	548778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68922393373103	19.602239015348385	12.514752756224215	24.193784294696368
2	14.951701197788218	21.240526287224483	40.324095495576714	23.48367701941059
3	18.64418940946606	25.518451196043728	29.00383043076708	26.833528963723136
4	11.39693976683938	17.531113842439943	38.01850270843147	33.05344368228921
5	12.947166366684703	39.07900015827269	34.27128307623223	13.702550398810379
6	31.136152551094142	37.896881101127875	18.55976332341351	12.40720302436447
7	29.08651543839744	31.97555126608857	21.36096233045759	17.5769709650564
8	26.528618133259656	35.663949900999214	20.055348344614817	17.752083621126307
9	26.718815047322213	15.397495551453929	20.5776110110265	37.30607839019736
10	15.682851765910977	29.406486997357188	32.12317391420495	22.787487322526893
11	34.79019899933762	23.430442356890634	23.093541020063917	18.68581762370783
12	23.14632885926442	26.604267360852035	30.152940016894053	20.09646376298949
13	28.197712172975127	22.00479155978074	26.07319609564329	23.72430017160084
14	21.732327120948103	22.561402410046895	27.14802521302501	28.558245255979987
15	23.24925017870434	30.9722252927435	23.27660852327578	22.501916005276385
16	22.798733014837065	28.875859216911802	26.605398963091094	21.720008805160045
17	21.76402321083172	29.393663074514137	26.573454913880447	22.268858800773693
18	21.90002385252326	27.860944394150515	28.999787261279064	21.239244492047163
19	23.171640176171536	27.43043885424043	27.6871638426611	21.710757126926932
20	24.356923915515498	26.668471340218712	27.395773196730595	21.578831547535195
21	25.325392791638123	26.591607186263893	26.206181542291855	21.876818479806126
22	24.200263179462056	27.72703372435773	26.99863990180036	21.074063194379857
23	22.427898292538764	27.99569866338174	27.461253905911942	22.11514913816755
24	22.607100732282007	27.570560292875975	27.9404303825674	21.881908592274623
25	22.31798658521427	27.748166630605414	27.43052070913301	22.503326075047305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	66.0
2	226.5
3	387.0
4	387.0
5	387.0
6	794.5
7	1202.0
8	1202.0
9	1202.0
10	1675.0
11	2148.0
12	2148.0
13	2148.0
14	3456.5
15	4765.0
16	4765.0
17	4765.0
18	8222.5
19	11680.0
20	11680.0
21	11680.0
22	18268.0
23	24856.0
24	24856.0
25	24856.0
26	36078.5
27	47301.0
28	47301.0
29	47301.0
30	60556.0
31	73811.0
32	73811.0
33	73811.0
34	87590.0
35	101369.0
36	101369.0
37	101369.0
38	112353.5
39	123338.0
40	123338.0
41	123338.0
42	133061.0
43	142784.0
44	142784.0
45	142784.0
46	147609.0
47	152434.0
48	152434.0
49	152434.0
50	147787.0
51	143140.0
52	143140.0
53	143140.0
54	120784.5
55	98429.0
56	98429.0
57	98429.0
58	82896.0
59	67363.0
60	67363.0
61	67363.0
62	55034.0
63	42705.0
64	42705.0
65	42705.0
66	33294.0
67	23883.0
68	23883.0
69	23883.0
70	17596.5
71	11310.0
72	11310.0
73	11310.0
74	9021.5
75	6733.0
76	6733.0
77	6733.0
78	5559.0
79	4385.0
80	4385.0
81	4385.0
82	2941.5
83	1498.0
84	1498.0
85	1498.0
86	1141.5
87	785.0
88	785.0
89	785.0
90	543.0
91	301.0
92	301.0
93	301.0
94	183.5
95	66.0
96	66.0
97	66.0
98	211.0
99	356.0
100	356.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.198828069303971E-4
2	7.359062455443177E-4
3	0.0010118710876234368
4	0.010946605402471724
5	0.03339174589157341
6	0.06604758553760251
7	0.11323757353313188
8	0.11452540946283443
9	0.12390821409352448
10	0.1387183272851039
11	0.1484690850385661
12	0.1385343507237178
13	0.13209517107520502
14	0.1202286828658029
15	0.1382583858816387
16	0.12583996798807834
17	0.12832365156679038
18	0.11553728055045787
19	0.101923015007888
20	0.09437997599105874
21	0.09410401114897962
22	0.10523459311283742
23	0.08472120651828957
24	0.09548383535937521
25	0.09014851507917891
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1087095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.04112337983906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.58485954590529	35.516894014513234
2	16.767011430616854	17.116141982825734
3	6.432892049938276	9.850261104302561
4	2.970599212779188	6.064908837260612
5	1.5043212506722856	3.839112327923897
6	0.8462060034570094	2.591478301632584
7	0.5027204450262129	1.7961591382105375
8	0.30214121433038715	1.233730159901735
9	0.20871017150427545	0.9587521452939384
>10	0.730338991023755	6.435145823730523
>50	0.06751116965860074	2.440487899985648
>100	0.07165131886845598	7.563771426196597
>500	0.008142193932336353	2.810289787616527
>1k	0.002895002287052925	1.7828670506058841
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	1919	0.1765255106499432	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1454	0.13375096012767973	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1353	0.12446014377768273	No Hit
TCGTAGTTCCGACCATAAACGATGC	1281	0.11783698756778387	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1249	0.11489336258560659	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1238	0.11388149149798316	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1238	0.11388149149798316	No Hit
GTATCAACGCAGAGTACATGGGGAA	1161	0.10679839388461909	No Hit
GGTATCAACGCAGAGTACATGGGGA	1112	0.10229096813066016	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1098	0.1010031322009576	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1088	0.1000832493940272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTAC	30	7.7475375E-4	18.990192	1
GCTCGTA	40	2.7574488E-4	16.627136	9
ATTGGAC	40	2.7622987E-4	16.623308	3
GGTATCA	680	0.0	16.33715	1
CTCGTAG	45	6.748702E-4	14.78104	10
CCGTCCT	40	0.005273246	14.251832	9
AGGTCGT	40	0.005273246	14.251832	6
CGGTCCA	130	1.8189894E-12	13.88768	10
CGCCGGT	140	0.0	13.573797	7
GCCGGTC	140	0.0	13.573173	8
CCGACCA	260	0.0	13.155538	9
CGACCAT	255	0.0	13.042093	10
CGGACCA	270	0.0	13.020191	9
GTATCAA	2170	0.0	12.951837	1
AGCCCCG	235	0.0	12.935449	16
CCGGTCC	140	3.6379788E-12	12.894514	9
AGGCCCG	195	0.0	12.669462	10
CGTCGTA	195	0.0	12.669462	10
GGTCGTG	60	4.0842398E-4	12.668878	7
ACGATCA	75	1.4756592E-5	12.668295	9
>>END_MODULE