FastQCFastQC Report
Thu 2 Feb 2017
SRR4062049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087095
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19010.17486972159746847No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13870.1275877453212461No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13040.11995271802372379No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12550.11544529226976484No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12090.11121383135788501No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11950.10992599542818246No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11390.10477465170937222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.033.6742971
ACGGTCG300.005745466329.33259812
GTATCAA22150.027.0263121
CGCGGGA1301.8189894E-1223.69389344
TAAACGC1254.0017767E-1122.88047828
GTAAACG1254.0017767E-1122.88047827
CGCTTCG1450.022.75804932
GCTTCGG1750.021.37089233
CGGGCCC1451.2732926E-1121.24084737
AACCGCG1158.76571E-921.04391941
TCGGCGC1158.771167E-921.04295218
GTAGGAC7100.020.7600443
CCCCGCG1502.1827873E-1120.53376241
AAACGCT1502.1827873E-1120.53281829
GCGCCCC1301.6243575E-920.30718221
GCCGATC1201.4315447E-820.166168
CGCCCCT1201.4315447E-820.166165
ATACGAA2550.019.84355240
CGATCGC1001.1234933E-619.79950310
CGCGGTC1252.2855602E-819.36129644
Produced by FastQC (version 0.11.5)