##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1087095
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31441042411197	32.0	32.0	32.0	32.0	32.0
2	31.50126621868374	32.0	32.0	32.0	32.0	32.0
3	31.61204678523956	32.0	32.0	32.0	32.0	32.0
4	31.67500540431149	32.0	32.0	32.0	32.0	32.0
5	31.665881086749547	32.0	32.0	32.0	32.0	32.0
6	35.310994899249835	36.0	36.0	36.0	36.0	36.0
7	35.30967210777347	36.0	36.0	36.0	36.0	36.0
8	35.25491240415971	36.0	36.0	36.0	36.0	36.0
9	35.34206670070233	36.0	36.0	36.0	36.0	36.0
10	35.23914009355208	36.0	36.0	36.0	36.0	36.0
11	35.349135080190784	36.0	36.0	36.0	36.0	36.0
12	35.27219976175035	36.0	36.0	36.0	36.0	36.0
13	35.303247646249865	36.0	36.0	36.0	36.0	36.0
14	35.26396405097991	36.0	36.0	36.0	36.0	36.0
15	35.25913834577475	36.0	36.0	36.0	36.0	36.0
16	35.268301298414585	36.0	36.0	36.0	36.0	36.0
17	35.256598549344815	36.0	36.0	36.0	36.0	36.0
18	35.247832986077576	36.0	36.0	36.0	36.0	36.0
19	35.24133125439819	36.0	36.0	36.0	36.0	36.0
20	35.23842994402513	36.0	36.0	36.0	36.0	36.0
21	35.22876749502113	36.0	36.0	36.0	36.0	36.0
22	35.210988000128786	36.0	36.0	36.0	36.0	36.0
23	35.18558267676698	36.0	36.0	36.0	36.0	36.0
24	35.17692933920218	36.0	36.0	36.0	36.0	36.0
25	35.150135912684725	36.0	36.0	36.0	36.0	36.0
26	35.110795284680734	36.0	36.0	36.0	36.0	36.0
27	35.091501662688174	36.0	36.0	36.0	36.0	36.0
28	35.069995722544945	36.0	36.0	36.0	36.0	36.0
29	35.05173604882738	36.0	36.0	36.0	36.0	36.0
30	35.03173871648752	36.0	36.0	36.0	36.0	36.0
31	35.03085930852409	36.0	36.0	36.0	36.0	36.0
32	35.00479534907253	36.0	36.0	36.0	36.0	36.0
33	34.992934380159966	36.0	36.0	36.0	36.0	36.0
34	34.98249003077008	36.0	36.0	36.0	36.0	36.0
35	34.97554951499179	36.0	36.0	36.0	36.0	36.0
36	34.946042434193885	36.0	36.0	36.0	36.0	36.0
37	34.92331121015183	36.0	36.0	36.0	36.0	36.0
38	34.90676067868954	36.0	36.0	36.0	36.0	36.0
39	34.85794249812574	36.0	36.0	36.0	36.0	36.0
40	34.86862509716262	36.0	36.0	36.0	36.0	36.0
41	34.84109116498558	36.0	36.0	36.0	36.0	36.0
42	34.81665355833667	36.0	36.0	36.0	32.0	36.0
43	34.813364057419086	36.0	36.0	36.0	32.0	36.0
44	34.75999245696098	36.0	36.0	36.0	32.0	36.0
45	34.763231364324184	36.0	36.0	36.0	32.0	36.0
46	34.71531650867679	36.0	36.0	36.0	32.0	36.0
47	34.70594290287417	36.0	36.0	36.0	32.0	36.0
48	34.67829490522907	36.0	36.0	36.0	32.0	36.0
49	34.6489644419301	36.0	36.0	36.0	32.0	36.0
50	34.268425482593514	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	5.0
21	16.0
22	69.0
23	193.0
24	523.0
25	1202.0
26	2512.0
27	4773.0
28	7965.0
29	12981.0
30	19498.0
31	28811.0
32	43073.0
33	71724.0
34	162847.0
35	730899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.417408091398364	20.060289355781734	12.967824513070514	24.554478039749384
2	14.582417238708349	21.687754474988086	40.070576849836534	23.659251436467034
3	17.825814190519292	25.88106618391084	29.431639640435215	26.86147998513465
4	11.211531650867679	17.964483324824418	38.0982342849521	32.72575073935581
5	12.63780994301326	39.22941417263441	34.25983929647363	13.872936587878703
6	31.12843151413908	38.1992157064759	18.371841231614958	12.30051154777006
7	29.04272395696788	31.998307415635246	21.31350066001591	17.645467967380956
8	26.432924445425655	35.893275196739935	19.945450949549027	17.728349408285386
9	26.765278103569607	15.2170693453654	20.491309407181525	37.526343143883466
10	15.63930994007878	29.342356778714628	32.24118613477767	22.77714714642892
11	34.95922619458281	23.35913604606773	23.155841945736114	18.525795813613346
12	23.216094269590055	26.540826698678586	30.07694819679973	20.166130834931632
13	28.244633633675072	21.916023898555324	26.12807528320892	23.711267184560686
14	21.75789604404399	22.3762412668626	27.1406822770779	28.72518041201551
15	23.250865839692022	30.929587570543514	23.222073507835102	22.597473081929362
16	22.809234963931097	28.912339962063722	26.547761630634557	21.730663443370627
17	21.833694387335054	29.291828221084632	26.59528376084887	22.279193630731445
18	22.119982264643166	27.791340951196492	28.92702930933296	21.161647474827383
19	23.31249798775636	27.277468850468452	27.624908586646065	21.785124575129128
20	24.572643872500095	26.489318741485302	27.339937502932138	21.598099883082465
21	25.360617057386893	26.39842884016576	26.169561997801484	22.071392104645867
22	24.24691494303626	27.76454679673809	26.936376305658662	21.05216195456699
23	22.513487781656615	27.9283779246524	27.431641208909983	22.126493084781
24	22.640782200504653	27.448953027678556	27.986720474074296	21.9235442977425
25	22.505086965960565	27.443767408830162	27.4888417907592	22.562303834450073
26	21.657484686222798	28.596357441359665	28.07073543845835	21.675422433959195
27	22.59098347611474	27.714372183428647	27.45064129747869	22.244003042977926
28	21.74394590989582	27.963663960628292	27.882712784306513	22.409677345169378
29	22.177239828827737	27.747329572842037	27.83076317367803	22.244667424652192
30	21.78002967574693	27.912128144769078	28.382009469378144	21.92583271010585
31	22.95561816925753	27.487445830102224	27.339711835921438	22.217224164718804
32	22.54949939471099	27.795184180799275	27.41278502857185	22.242531395917887
33	21.851672304025506	27.875259982356614	27.99916842441263	22.27389928920525
34	22.64761021667099	27.99585313397468	28.07312392947752	21.283412719876807
35	22.84153900214701	27.711403061740754	28.060038966271545	21.387018969840693
36	21.39870149223526	28.040813397921433	28.25284657996456	22.30763852987874
37	22.41000407514831	27.74513672904495	27.480114104152698	22.364745091654044
38	21.644106540826698	27.76951416389552	27.942176166756354	22.644203128521426
39	23.35956242732879	27.407624773707372	27.26761770896192	21.965195090001913
40	23.09367911146598	27.23462736433096	28.088647347883438	21.58304617631962
41	22.40173455335895	27.912547455065447	28.192011450848646	21.493706540726958
42	22.87179336217	28.1307437983077	27.831123360458932	21.166339479063367
43	22.39949956764116	27.46582525343587	28.0911817195003	22.043493459422663
44	21.838669141848918	28.075778845287005	27.73458269935919	22.35096931350489
45	22.379187490858513	28.15449135617808	27.469259307298376	21.997061845665026
46	21.8094192906233	27.82385998287134	27.748979593714097	22.617741132791263
47	22.364529663328376	27.27243457951233	28.025458957595696	22.337576799563603
48	22.15556853672111	28.238021425055997	27.355997221826144	22.250412816396743
49	22.28180226723076	28.111876789816137	27.25368496292269	22.352635980030414
50	22.21466878857847	28.425860340180485	27.2888590431159	22.070611828125145
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	15.0
4	28.0
5	40.0
6	52.0
7	72.5
8	93.0
9	143.5
10	194.0
11	282.0
12	370.0
13	677.5
14	985.0
15	1391.5
16	1798.0
17	2075.0
18	2352.0
19	2795.0
20	3238.0
21	3993.5
22	4749.0
23	6055.0
24	7361.0
25	9460.5
26	11560.0
27	14601.5
28	17643.0
29	21975.5
30	26308.0
31	31489.0
32	36670.0
33	43001.5
34	49333.0
35	55172.0
36	61011.0
37	66320.0
38	71629.0
39	73759.0
40	75889.0
41	77990.0
42	80091.0
43	81174.5
44	82258.0
45	83613.5
46	84969.0
47	84840.5
48	84712.0
49	84494.5
50	84277.0
51	75454.5
52	66632.0
53	62282.5
54	57933.0
55	54029.5
56	50126.0
57	44967.0
58	39808.0
59	34213.5
60	28619.0
61	23214.5
62	17810.0
63	14474.5
64	11139.0
65	9208.5
66	7278.0
67	6806.5
68	6335.0
69	6091.5
70	5848.0
71	4115.5
72	2383.0
73	2251.5
74	2120.0
75	1526.5
76	933.0
77	900.5
78	868.0
79	738.0
80	608.0
81	484.5
82	361.0
83	339.5
84	318.0
85	277.0
86	236.0
87	171.5
88	107.0
89	71.5
90	36.0
91	22.5
92	9.0
93	6.5
94	4.0
95	6.0
96	8.0
97	5.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03090806231286134
2	0.004875378876731105
3	2.759648420791191E-4
4	0.0
5	0.0
6	1.8397656138607942E-4
7	0.0
8	0.0
9	0.0
10	9.198828069303971E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.278945262373574E-4
17	0.0
18	9.198828069303971E-5
19	0.0
20	3.6795312277215883E-4
21	0.0
22	0.0
23	0.0
24	7.359062455443177E-4
25	2.759648420791191E-4
26	1.8397656138607942E-4
27	1.8397656138607942E-4
28	0.0018397656138607942
29	2.759648420791191E-4
30	0.0011038593683164765
31	5.519296841582382E-4
32	0.0010118710876234368
33	1.8397656138607942E-4
34	8.278945262373574E-4
35	9.198828069303971E-5
36	9.198828069303971E-5
37	0.0016557890524747149
38	0.0
39	6.439179648512779E-4
40	9.198828069303971E-5
41	0.0012878359297025558
42	0.004875378876731105
43	0.00321958982425639
44	0.0034035663856424687
45	0.0016557890524747149
46	0.0025756718594051116
47	0.0011038593683164765
48	0.004599414034651986
49	0.0038635077891076676
50	0.002299707017325993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1087095.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.90311044226429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.33515362618094	37.373804434433936
2	17.00466770227471	18.332089623794364
3	6.550842703446694	10.59332393201365
4	3.044589245029593	6.564513213046408
5	1.5152679859600313	4.083882879841547
6	0.8403423138368874	2.7178238731234594
7	0.48512623260832266	1.830486902630821
8	0.30548335902033164	1.3173202591637447
9	0.20708905335692634	1.0046469703034129
>10	0.6034243875993116	5.4316876847053885
>50	0.04964033583277935	1.9175788231394153
>100	0.05184900953933604	5.861723591066779
>500	0.004978876687135935	1.9101034622299764
>1k	0.0015451686270421866	1.0610143505071066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1901	0.17486972159746847	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1387	0.1275877453212461	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1304	0.11995271802372379	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1255	0.11544529226976484	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1209	0.11121383135788501	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1195	0.10992599542818246	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1139	0.10477465170937222	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	9.198828069303971E-5	0.0
23	0.0	0.0	0.0	9.198828069303971E-5	0.0
24	0.0	0.0	0.0	3.6795312277215883E-4	0.0
25	0.0	0.0	0.0	3.6795312277215883E-4	0.0
26	0.0	0.0	0.0	3.6795312277215883E-4	0.0
27	0.0	0.0	0.0	3.6795312277215883E-4	0.0
28	0.0	0.0	0.0	0.0011038593683164765	0.0
29	0.0	0.0	0.0	0.0023916952980190323	0.0
30	0.0	0.0	0.0	0.004507425753958945	0.0
31	0.0	0.0	0.0	0.010670640560392606	0.0
32	0.0	0.0	0.0	0.019501515506924416	0.0
33	0.0	0.0	0.0	0.02824040217276319	0.0
34	0.0	0.0	0.0	0.038451101329690596	0.0
35	0.0	0.0	0.0	0.04856981220592497	0.0
36	0.0	0.0	0.0	0.0653116792920582	0.0
37	0.0	0.0	0.0	0.08840073774601116	0.0
38	0.0	0.0	0.0	0.12133254223411938	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	915	0.0	33.674297	1
ACGGTCG	30	0.0057454663	29.332598	12
GTATCAA	2215	0.0	27.026312	1
CGCGGGA	130	1.8189894E-12	23.693893	44
TAAACGC	125	4.0017767E-11	22.880478	28
GTAAACG	125	4.0017767E-11	22.880478	27
CGCTTCG	145	0.0	22.758049	32
GCTTCGG	175	0.0	21.370892	33
CGGGCCC	145	1.2732926E-11	21.240847	37
AACCGCG	115	8.76571E-9	21.043919	41
TCGGCGC	115	8.771167E-9	21.042952	18
GTAGGAC	710	0.0	20.760044	3
CCCCGCG	150	2.1827873E-11	20.533762	41
AAACGCT	150	2.1827873E-11	20.532818	29
GCGCCCC	130	1.6243575E-9	20.307182	21
GCCGATC	120	1.4315447E-8	20.16616	8
CGCCCCT	120	1.4315447E-8	20.16616	5
ATACGAA	255	0.0	19.843552	40
CGATCGC	100	1.1234933E-6	19.799503	10
CGCGGTC	125	2.2855602E-8	19.361296	44
>>END_MODULE