FastQCFastQC Report
Thu 2 Feb 2017
SRR4062048_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062048_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234750
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG19310.822577209797657No Hit
GTCCTAAAGTGTGTATTTCTCATTT14670.6249201277955272No Hit
TCCATGTACTCTGCGTTGATACCAC14640.6236421725239616No Hit
GTCCTACAGTGGACATTTCTAAATT13350.5686900958466454No Hit
CTGTAGGACGTGGAATATGGCAAGA13210.5627263045793397No Hit
CTTTAGGACGTGAAATATGGCGAGG12680.5401490947816826No Hit
GTCCTACAGTGTGCATTTCTCATTT9380.3995740149094782No Hit
GAGTACATGGAAGCAGTGGTATCAA8040.3424920127795527No Hit
GTACATGGGAAGCAGTGGTATCAAC8010.3412140575079872No Hit
CTGTAGGACCTGGAATATGGCGAGA7500.3194888178913738No Hit
GTATCAACGCAGAGTACTTTTTTTT6880.2930777422790202No Hit
CCCATGTACTCTGCGTTGATACCAC6700.2854100106496273No Hit
CTGAAGGACCTGGAATATGGCGAGA6210.2645367412140575No Hit
CATGTACTCTGCGTTGATACCACTG5770.2457933972310969No Hit
GTCCTTCAGTGTGCATTTCTCATTT5670.24153354632587862No Hit
ATTTAGAAATGTCCACTGTAGGACG5380.22917997870074547No Hit
GCTTCCATGTACTCTGCGTTGATAC5150.21938232161874335No Hit
TATCAACGCAGAGTACTTTTTTTTT4800.20447284345047922No Hit
TTTCTAAATTTTCCACCTTTTTCAG4430.18871139510117146No Hit
CATGGAAGCAGTGGTATCAACGCAG4410.1878594249201278No Hit
GCGTTGATACCACTGCTTCCATGTA4270.18189563365282216No Hit
GTATCAACGCAGAGTACATGGGAAG4220.17976570820021298No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4180.17806176783812566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4150.17678381256656017No Hit
GAATATGGCAAGAAAACTGAAAATC4090.1742279020234292No Hit
GGTATCAACGCAGAGTACATGGGAA3480.14824281150159743No Hit
TTGTAGAACAGTGTATATCAATGAG3340.1422790202342918No Hit
GTATCAACGCAGAGTACATGGAAGC3290.14014909478168264No Hit
GATATACACTGTTCTACAAATCCCG3280.1397231096911608No Hit
GAAATATGGCGAGGAAAACTGAAAA3160.13461128860489882No Hit
GGAATATGGCGAGAAAACTGAAAAT3080.13120340788072415No Hit
ATTCCAGGTCCTTCAGTGTGCATTT3050.1299254526091587No Hit
GTGTATATCAATGAGTTACAATGAA3000.12779552715654952No Hit
CCATATTCCAGGTCCTTCAGTGTGC2800.11927582534611289No Hit
ACCTGGAATATGGCGAGAAAACTGA2770.1179978700745474No Hit
CTGTAGGACATGGAATATGGCAAGA2730.11629392971246007No Hit
GGTATCAACGCAGAGTACTTTTTTT2720.11586794462193824No Hit
GAGTACATGGGAAGCAGTGGTATCA2690.11458998935037273No Hit
TATCAACGCAGAGTACATGGGAAGC2680.1141640042598509No Hit
GACCTGGAATATGGCGAGAAAACTG2660.11331203407880724No Hit
ACTCTGCGTTGATACCACTGCTTCC2630.11203407880724175No Hit
CCATATTTCACGTCCTAAAGTGTGT2590.11033013844515442No Hit
GTGTATATCAATGAGTTACAATGAG2580.10990415335463259No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2520.1073482428115016No Hit
ACAGTGGACATTTCTAAATTTTCCA2510.10692225772097977No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2490.10607028753993611No Hit
CAGTGGTATCAACGCAGAGTACATG2480.10564430244941427No Hit
ATACACACTTTAGGACGTGAAATAT2480.10564430244941427No Hit
TTCCAGGTCCTTCAGTGTGCATTTC2460.1047923322683706No Hit
GTCCACTGTAGGACGTGGAATATGG2460.1047923322683706No Hit
GCCATATTCCACGTCCTACAGTGGA2410.10266240681576144No Hit
CCACTGTAGGACGTGGAATATGGCA2390.10181043663471778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG250.006020271718.99829515
TTACCGC250.006020271718.99829516
CTTTTAA350.002164116416.2842522
TGATATA508.6704036E-515.1986362
TCCAACG653.3458364E-614.61407218
CGGTTTC400.00525712414.2517613
GAACAGT602.5469953E-514.2517596
GAATAAC400.00527202714.2456841
TTGATAT551.9540255E-413.8139951
CACTGTG707.1906707E-613.5731047
TAGGACC2100.013.57020954
CATCTAA707.2224466E-613.5673181
GCCGGTT500.001491545913.30164111
CCGGTTT500.001491545913.30164112
AACCACA500.001493946413.2988072
AATATTC655.4078086E-513.155477
TTCCACC1550.012.87255811
AGTGTAT751.4640889E-512.6682310
AAATGTC1700.012.2956357
AAATTTA853.907073E-612.29301415
Produced by FastQC (version 0.11.5)