##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062048_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234750
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23241746538871	32.0	32.0	32.0	32.0	32.0
2	30.954287539936104	32.0	32.0	32.0	32.0	32.0
3	30.98886474973376	32.0	32.0	32.0	32.0	32.0
4	30.964102236421724	32.0	32.0	32.0	32.0	32.0
5	30.99586794462194	32.0	32.0	32.0	32.0	32.0
6	34.623361022364215	36.0	36.0	36.0	32.0	36.0
7	34.53081576144835	36.0	36.0	36.0	32.0	36.0
8	34.48213844515442	36.0	36.0	36.0	32.0	36.0
9	34.50700745473908	36.0	36.0	36.0	32.0	36.0
10	34.33010436634718	36.0	36.0	36.0	32.0	36.0
11	34.53097337593184	36.0	36.0	36.0	32.0	36.0
12	34.38595101171459	36.0	36.0	36.0	32.0	36.0
13	34.405171458998936	36.0	36.0	36.0	32.0	36.0
14	34.343101171459	36.0	36.0	36.0	32.0	36.0
15	34.320668796592116	36.0	36.0	36.0	32.0	36.0
16	34.29468370607029	36.0	36.0	36.0	32.0	36.0
17	34.26215548455804	36.0	36.0	36.0	32.0	36.0
18	34.240149094781685	36.0	36.0	36.0	32.0	36.0
19	34.19072204472843	36.0	36.0	36.0	32.0	36.0
20	34.14307135250266	36.0	36.0	36.0	32.0	36.0
21	34.031816826411074	36.0	36.0	36.0	32.0	36.0
22	33.979987220447285	36.0	36.0	36.0	32.0	36.0
23	33.94572097976571	36.0	36.0	36.0	32.0	36.0
24	33.87301384451544	36.0	36.0	36.0	32.0	36.0
25	33.45392971246007	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	6.0
5	17.0
6	40.0
7	10.0
8	38.0
9	41.0
10	16.0
11	0.0
12	9.0
13	21.0
14	86.0
15	105.0
16	160.0
17	191.0
18	218.0
19	328.0
20	435.0
21	586.0
22	798.0
23	1018.0
24	1315.0
25	1713.0
26	2407.0
27	3013.0
28	3960.0
29	5464.0
30	7165.0
31	9929.0
32	14394.0
33	20368.0
34	46511.0
35	114387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44914546662804	18.870874299248555	12.3724163784143	25.307563855709102
2	12.684558040468582	20.6790202342918	44.14483493077742	22.491586794462194
3	19.369789603275013	26.146447025947083	29.240842268484794	25.24292110229311
4	11.279864353634846	17.70091339763471	37.68574690705838	33.33347534167206
5	11.322081275593034	39.54270228350584	35.50917620790599	13.626040232995146
6	30.513806361412094	36.85134824085046	19.08892507310253	13.545920324634913
7	27.111850240927893	31.88435461174364	22.144045030062685	18.85975011726579
8	24.625882252596114	39.80211953856323	19.891251039512124	15.68074716932853
9	26.67152867518798	15.951652869651259	19.982002038631645	37.394816416529125
10	16.41912642893704	28.74509469373827	33.20124552124211	21.63453335608258
11	33.62482297336496	21.11906427559848	26.25241012165782	19.003702629378743
12	25.893759784328864	24.044175045109224	31.669446446928944	18.392618723632967
13	31.08997462199569	22.38723848926233	25.291420528459618	21.231366360282358
14	20.838628783937086	24.66030928274721	25.70902174191182	28.792040191403885
15	22.237625029324576	34.98944315297831	23.15937639958199	19.613555418115123
16	20.570034587614135	26.901145954613888	32.15240727235507	20.376412185416907
17	20.187669275553944	29.998933697297993	29.830884391461048	19.98251263568702
18	21.062282777884388	25.146378387598883	35.254909486342996	18.536429348173737
19	24.486088903102015	26.879437160217464	26.517002451764206	22.117471484916322
20	24.204579371508974	27.115933995650877	29.79448258218565	18.8850040506545
21	22.09748048215786	26.94123114838677	25.327358237147646	25.633930132307732
22	23.365215277748167	31.584019512943957	25.49176797676868	19.5589972325392
23	19.42793557133965	31.03549715630516	28.329510475199744	21.207056797155452
24	23.46462803151755	28.558941910836715	28.62545621994065	19.350973837705084
25	21.93025466979905	28.813118500547887	28.703541841655333	20.55308498799773
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	493.0
1	493.0
2	363.5
3	234.0
4	234.0
5	234.0
6	509.5
7	785.0
8	785.0
9	785.0
10	756.0
11	727.0
12	727.0
13	727.0
14	783.0
15	839.0
16	839.0
17	839.0
18	1512.5
19	2186.0
20	2186.0
21	2186.0
22	3988.5
23	5791.0
24	5791.0
25	5791.0
26	9560.5
27	13330.0
28	13330.0
29	13330.0
30	17911.5
31	22493.0
32	22493.0
33	22493.0
34	23510.5
35	24528.0
36	24528.0
37	24528.0
38	24970.5
39	25413.0
40	25413.0
41	25413.0
42	26741.0
43	28069.0
44	28069.0
45	28069.0
46	32802.5
47	37536.0
48	37536.0
49	37536.0
50	31697.5
51	25859.0
52	25859.0
53	25859.0
54	20517.0
55	15175.0
56	15175.0
57	15175.0
58	13250.0
59	11325.0
60	11325.0
61	11325.0
62	9872.5
63	8420.0
64	8420.0
65	8420.0
66	6884.0
67	5348.0
68	5348.0
69	5348.0
70	4084.5
71	2821.0
72	2821.0
73	2821.0
74	2183.5
75	1546.0
76	1546.0
77	1546.0
78	1301.5
79	1057.0
80	1057.0
81	1057.0
82	727.5
83	398.0
84	398.0
85	398.0
86	289.0
87	180.0
88	180.0
89	180.0
90	129.5
91	79.0
92	79.0
93	79.0
94	49.5
95	20.0
96	20.0
97	20.0
98	59.0
99	98.0
100	98.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.519701810436635E-4
2	0.0
3	0.0012779552715654952
4	0.009371671991480297
5	0.028541001064962726
6	0.0630457933972311
7	0.10223642172523961
8	0.11288604898828541
9	0.11884984025559106
10	0.13205537806176784
11	0.1371671991480298
12	0.13588924387646434
13	0.12566560170394037
14	0.11586794462193824
15	0.1299254526091587
16	0.11629392971246007
17	0.12566560170394037
18	0.10862619808306709
19	0.09584664536741214
20	0.09371671991480299
21	0.09414270500532482
22	0.10181043663471778
23	0.08349307774227901
24	0.091160809371672
25	0.08988285410010649
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	234750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.68765404730446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.49123819480243	37.90750666825613
2	12.472059000084338	11.89526469707184
3	2.964272230505198	4.240775658910902
4	1.2886279534787317	2.4580657616471884
5	0.7135771927836243	1.701441115275608
6	0.44404423909722573	1.270525683346023
7	0.3754618798199886	1.2533427362964353
8	0.2582482039725737	0.985220080750545
9	0.2065974791826352	0.8866934202876025
>10	1.5018025272776638	14.267595615005572
>50	0.16686693927011795	5.65360922133055
>100	0.10031881468024947	9.780611328020655
>500	0.010925811499829153	3.537768705941511
>1k	0.005959533545361355	4.1615793078594345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	1931	0.822577209797657	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1467	0.6249201277955272	No Hit
TCCATGTACTCTGCGTTGATACCAC	1464	0.6236421725239616	No Hit
GTCCTACAGTGGACATTTCTAAATT	1335	0.5686900958466454	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1321	0.5627263045793397	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1268	0.5401490947816826	No Hit
GTCCTACAGTGTGCATTTCTCATTT	938	0.3995740149094782	No Hit
GAGTACATGGAAGCAGTGGTATCAA	804	0.3424920127795527	No Hit
GTACATGGGAAGCAGTGGTATCAAC	801	0.3412140575079872	No Hit
CTGTAGGACCTGGAATATGGCGAGA	750	0.3194888178913738	No Hit
GTATCAACGCAGAGTACTTTTTTTT	688	0.2930777422790202	No Hit
CCCATGTACTCTGCGTTGATACCAC	670	0.2854100106496273	No Hit
CTGAAGGACCTGGAATATGGCGAGA	621	0.2645367412140575	No Hit
CATGTACTCTGCGTTGATACCACTG	577	0.2457933972310969	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	567	0.24153354632587862	No Hit
ATTTAGAAATGTCCACTGTAGGACG	538	0.22917997870074547	No Hit
GCTTCCATGTACTCTGCGTTGATAC	515	0.21938232161874335	No Hit
TATCAACGCAGAGTACTTTTTTTTT	480	0.20447284345047922	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	443	0.18871139510117146	No Hit
CATGGAAGCAGTGGTATCAACGCAG	441	0.1878594249201278	No Hit
GCGTTGATACCACTGCTTCCATGTA	427	0.18189563365282216	No Hit
GTATCAACGCAGAGTACATGGGAAG	422	0.17976570820021298	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	418	0.17806176783812566	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	415	0.17678381256656017	No Hit
GAATATGGCAAGAAAACTGAAAATC	409	0.1742279020234292	No Hit
GGTATCAACGCAGAGTACATGGGAA	348	0.14824281150159743	No Hit
TTGTAGAACAGTGTATATCAATGAG	334	0.1422790202342918	No Hit
GTATCAACGCAGAGTACATGGAAGC	329	0.14014909478168264	No Hit
GATATACACTGTTCTACAAATCCCG	328	0.1397231096911608	No Hit
GAAATATGGCGAGGAAAACTGAAAA	316	0.13461128860489882	No Hit
GGAATATGGCGAGAAAACTGAAAAT	308	0.13120340788072415	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	305	0.1299254526091587	No Hit
GTGTATATCAATGAGTTACAATGAA	300	0.12779552715654952	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	280	0.11927582534611289	No Hit
ACCTGGAATATGGCGAGAAAACTGA	277	0.1179978700745474	No Hit
CTGTAGGACATGGAATATGGCAAGA	273	0.11629392971246007	No Hit
GGTATCAACGCAGAGTACTTTTTTT	272	0.11586794462193824	No Hit
GAGTACATGGGAAGCAGTGGTATCA	269	0.11458998935037273	No Hit
TATCAACGCAGAGTACATGGGAAGC	268	0.1141640042598509	No Hit
GACCTGGAATATGGCGAGAAAACTG	266	0.11331203407880724	No Hit
ACTCTGCGTTGATACCACTGCTTCC	263	0.11203407880724175	No Hit
CCATATTTCACGTCCTAAAGTGTGT	259	0.11033013844515442	No Hit
GTGTATATCAATGAGTTACAATGAG	258	0.10990415335463259	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	252	0.1073482428115016	No Hit
ACAGTGGACATTTCTAAATTTTCCA	251	0.10692225772097977	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	249	0.10607028753993611	No Hit
CAGTGGTATCAACGCAGAGTACATG	248	0.10564430244941427	No Hit
ATACACACTTTAGGACGTGAAATAT	248	0.10564430244941427	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	246	0.1047923322683706	No Hit
GTCCACTGTAGGACGTGGAATATGG	246	0.1047923322683706	No Hit
GCCATATTCCACGTCCTACAGTGGA	241	0.10266240681576144	No Hit
CCACTGTAGGACGTGGAATATGGCA	239	0.10181043663471778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.259850905218317E-4
2	0.0	0.0	0.0	0.0	4.259850905218317E-4
3	0.0	0.0	0.0	0.0	4.259850905218317E-4
4	0.0	0.0	0.0	0.0	4.259850905218317E-4
5	0.0	0.0	0.0	0.0	4.259850905218317E-4
6	0.0	0.0	0.0	0.0	4.259850905218317E-4
7	0.0	0.0	0.0	0.0	4.259850905218317E-4
8	0.0	0.0	0.0	0.0	4.259850905218317E-4
9	0.0	0.0	0.0	0.0	4.259850905218317E-4
10	0.0	0.0	0.0	4.259850905218317E-4	4.259850905218317E-4
11	0.0	0.0	0.0	4.259850905218317E-4	4.259850905218317E-4
12	0.0	0.0	0.0	4.259850905218317E-4	4.259850905218317E-4
13	0.0	0.0	0.0	4.259850905218317E-4	4.259850905218317E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACCG	25	0.0060202717	18.998295	15
TTACCGC	25	0.0060202717	18.998295	16
CTTTTAA	35	0.0021641164	16.284252	2
TGATATA	50	8.6704036E-5	15.198636	2
TCCAACG	65	3.3458364E-6	14.614072	18
CGGTTTC	40	0.005257124	14.25176	13
GAACAGT	60	2.5469953E-5	14.251759	6
GAATAAC	40	0.005272027	14.245684	1
TTGATAT	55	1.9540255E-4	13.813995	1
CACTGTG	70	7.1906707E-6	13.573104	7
TAGGACC	210	0.0	13.5702095	4
CATCTAA	70	7.2224466E-6	13.567318	1
GCCGGTT	50	0.0014915459	13.301641	11
CCGGTTT	50	0.0014915459	13.301641	12
AACCACA	50	0.0014939464	13.298807	2
AATATTC	65	5.4078086E-5	13.15547	7
TTCCACC	155	0.0	12.872558	11
AGTGTAT	75	1.4640889E-5	12.66823	10
AAATGTC	170	0.0	12.295635	7
AAATTTA	85	3.907073E-6	12.293014	15
>>END_MODULE