FastQCFastQC Report
Thu 2 Feb 2017
SRR4062048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234750
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA6560.2794462193823216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.2223642172523962No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC5190.22108626198083067No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5180.22066027689030884No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC4850.20660276890308837No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA4750.20234291799787005No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4480.1908413205537806No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA3290.14014909478168264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.1329073482428115No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA2890.12310969116080939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.12012779552715655No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA2400.10223642172523961No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2390.10181043663471778No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA2370.10095846645367412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGACC301.2979427E-436.6666644
GGCGTAG250.002348945535.228
GTGCTAG250.002348945535.21
GGTCTAC250.002348945535.21
ATATGAG353.21003E-431.4285743
CAACGTA300.0057357129.33333230
ATTCCGA300.0057357129.3333326
AATTCCG300.0057357129.3333325
GCGTAGG300.0057357129.33333229
TAATCCT300.0057357129.3333324
CGCCCCT407.01515E-427.55
GGTCGTA450.001394863824.44444529
ATCGTAA450.001394863824.4444451
TATGAGA450.001394863824.44444544
CCCCCTT450.001394863824.44444528
ACTGTTC1350.024.4444458
GGTTACC450.001394863824.44444511
GATATAC1450.024.2758621
ATGCCGG551.5882908E-424.019
GTAAACC551.5882908E-424.033
Produced by FastQC (version 0.11.5)