##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234750
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14055378061768	32.0	32.0	32.0	32.0	32.0
2	31.524966986155484	32.0	32.0	32.0	32.0	32.0
3	31.626892438764642	32.0	32.0	32.0	32.0	32.0
4	31.71590202342918	32.0	32.0	32.0	32.0	32.0
5	31.70672630457934	32.0	32.0	32.0	32.0	32.0
6	35.37208945686901	36.0	36.0	36.0	36.0	36.0
7	35.368328008519704	36.0	36.0	36.0	36.0	36.0
8	35.33277529286475	36.0	36.0	36.0	36.0	36.0
9	35.384609158679446	36.0	36.0	36.0	36.0	36.0
10	35.30866027689031	36.0	36.0	36.0	36.0	36.0
11	35.375718849840254	36.0	36.0	36.0	36.0	36.0
12	35.32254739084132	36.0	36.0	36.0	36.0	36.0
13	35.32282002129926	36.0	36.0	36.0	36.0	36.0
14	35.285022364217255	36.0	36.0	36.0	36.0	36.0
15	35.27525878594249	36.0	36.0	36.0	36.0	36.0
16	35.268681576144836	36.0	36.0	36.0	36.0	36.0
17	35.25590628328008	36.0	36.0	36.0	36.0	36.0
18	35.22997657082002	36.0	36.0	36.0	36.0	36.0
19	35.1788583599574	36.0	36.0	36.0	36.0	36.0
20	35.16207028753993	36.0	36.0	36.0	36.0	36.0
21	35.104511182108624	36.0	36.0	36.0	36.0	36.0
22	35.02980191693291	36.0	36.0	36.0	36.0	36.0
23	34.9982066027689	36.0	36.0	36.0	36.0	36.0
24	34.96660702875399	36.0	36.0	36.0	36.0	36.0
25	34.870909478168265	36.0	36.0	36.0	36.0	36.0
26	34.821644302449414	36.0	36.0	36.0	32.0	36.0
27	34.709708200212994	36.0	36.0	36.0	32.0	36.0
28	34.63748668796592	36.0	36.0	36.0	32.0	36.0
29	34.58560170394036	36.0	36.0	36.0	32.0	36.0
30	34.48302875399361	36.0	36.0	36.0	32.0	36.0
31	34.475659211927585	36.0	36.0	36.0	32.0	36.0
32	34.41699254526092	36.0	36.0	36.0	32.0	36.0
33	34.43423642172524	36.0	36.0	36.0	32.0	36.0
34	34.36588711395101	36.0	36.0	36.0	32.0	36.0
35	34.296886048988284	36.0	36.0	36.0	32.0	36.0
36	34.198807241746536	36.0	36.0	36.0	32.0	36.0
37	33.98996805111821	36.0	36.0	36.0	32.0	36.0
38	33.94456230031949	36.0	36.0	36.0	32.0	36.0
39	33.58324600638978	36.0	36.0	36.0	21.0	36.0
40	33.65257507987221	36.0	36.0	36.0	27.0	36.0
41	33.64932055378062	36.0	36.0	36.0	27.0	36.0
42	33.51433439829606	36.0	36.0	36.0	21.0	36.0
43	33.56175505857295	36.0	36.0	36.0	21.0	36.0
44	33.459906283280084	36.0	36.0	36.0	21.0	36.0
45	33.28433652822151	36.0	36.0	36.0	14.0	36.0
46	33.22617678381257	36.0	36.0	36.0	14.0	36.0
47	33.17956549520767	36.0	36.0	36.0	14.0	36.0
48	33.18300319488818	36.0	36.0	36.0	14.0	36.0
49	33.14693503727369	36.0	36.0	36.0	14.0	36.0
50	32.78215974440894	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	12.0
21	17.0
22	53.0
23	111.0
24	296.0
25	746.0
26	1566.0
27	2805.0
28	4394.0
29	5767.0
30	7219.0
31	9439.0
32	13286.0
33	18273.0
34	37356.0
35	133407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01346571781651	19.35483870967742	12.969702134912856	25.661993437593217
2	12.354414633938537	21.082720603897044	43.58657590035017	22.976288861814247
3	19.017252396166135	26.308413205537807	29.708626198083067	24.965708200212994
4	11.186794462193824	17.89137380191693	37.93312034078807	32.98871139510117
5	11.125026624068157	39.82917997870074	35.30223642172524	13.743556975505857
6	31.167625133120342	36.689243876464325	18.638551650692225	13.504579339723112
7	27.02151224707135	31.858998935037274	22.364643237486685	18.754845580404687
8	24.647923322683706	39.64728434504792	20.097976570820023	15.60681576144835
9	26.505644302449415	15.404046858359957	20.238551650692227	37.851757188498404
10	16.270500532481364	28.46815761448349	33.659637912673055	21.60170394036209
11	33.642598509052185	20.739936102236424	26.69520766773163	18.922257720979765
12	25.586794462193822	23.92800851970181	32.31352502662407	18.1716719914803
13	31.294142705005324	21.95101171458999	25.707348242811502	21.047497337593185
14	20.833652822151226	24.065601703940363	26.2790202342918	28.82172523961661
15	22.170820021299253	34.73312034078807	23.642598509052185	19.45346112886049
16	20.6888178913738	26.324174653887113	32.27774227902023	20.709265175718848
17	19.985516506922256	29.79595314164004	30.309265175718853	19.90926517571885
18	21.242598509052183	24.659424920127794	35.78317358892439	18.314802981895635
19	24.51160809371672	26.40894568690096	27.211501597444087	21.867944621938232
20	24.140354763405867	26.722272394227005	30.4288002453695	18.70857259699763
21	21.895633652822152	26.448136315228965	26.17380191693291	25.482428115015978
22	23.295420660276893	30.702875399361023	26.459211927582533	19.54249201277955
23	19.415548455804046	29.996592119275828	29.11352502662407	21.47433439829606
24	23.559807960194767	27.588895089523437	29.459877397835076	19.391419552446717
25	22.45537806176784	27.747390841320552	29.12247071352503	20.67476038338658
26	18.26453674121406	29.363152289669863	31.22768903088392	21.14462193823216
27	21.154845580404686	29.629392971246006	28.984451544195956	20.231309904153356
28	19.709988114965345	28.91982500457936	27.826742605932292	23.543444274523
29	21.33290734824281	27.42747603833866	28.04472843450479	23.19488817891374
30	20.716683422222978	27.221957162574334	30.113568592703665	21.94779082249902
31	23.34568690095847	25.49946751863685	28.765921192758253	22.388924387646433
32	20.09422830342195	29.511094828944955	29.231647419158335	21.163029448474756
33	18.91246006389776	29.56208732694356	31.419808306709264	20.105644302449413
34	20.070799023638013	29.47488594200614	31.50769545344176	18.94661958091408
35	20.261641157150827	30.071693596138854	30.035484709200038	19.631180537510275
36	19.135250266240682	29.05814696485623	31.18594249201278	20.62066027689031
37	20.60124047915211	28.813451019816995	27.59128938265715	22.994019118373746
38	21.301810436634717	30.98658146964856	26.946964856230032	20.764643237486688
39	23.04693989699549	27.231870907828426	28.25254422846724	21.468644966708837
40	24.523109691160812	27.305218317358893	28.97550585729499	19.1961661341853
41	22.95118979663125	25.899908837637277	30.71958627623048	20.429315089500992
42	22.14960955306287	30.71260293697892	29.26839659013935	17.869390919818855
43	19.84535753083264	29.919270666922277	29.760368074637356	20.475003727607728
44	19.32878357999131	29.863081392872054	29.785122136168834	21.023012890967802
45	20.074037470287035	30.36771659580994	28.72636807442938	20.831877859473643
46	20.58403799867942	26.969264521076063	29.88434258450659	22.56235489573793
47	24.214902789374136	26.91950517150306	28.76744423807658	20.098147801046228
48	19.959188715978172	30.14965557491512	27.390846855443705	22.500308853663004
49	21.92212660816222	27.607991820737837	29.265144415097556	21.204737156002384
50	19.539510117145902	32.882641107561234	26.280298189563368	21.2975505857295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	131.5
2	71.0
3	197.5
4	324.0
5	292.5
6	261.0
7	196.0
8	131.0
9	169.0
10	207.0
11	324.0
12	441.0
13	674.0
14	907.0
15	1066.0
16	1225.0
17	1235.0
18	1245.0
19	1141.0
20	1037.0
21	1127.5
22	1218.0
23	1339.0
24	1460.0
25	1910.0
26	2360.0
27	3589.0
28	4818.0
29	6306.0
30	7794.0
31	9435.0
32	11076.0
33	13914.0
34	16752.0
35	19515.0
36	22278.0
37	21878.0
38	21478.0
39	19164.0
40	16850.0
41	15357.0
42	13864.0
43	12969.0
44	12074.0
45	12691.0
46	13308.0
47	13746.5
48	14185.0
49	13160.5
50	12136.0
51	10964.5
52	9793.0
53	9090.0
54	8387.0
55	8363.5
56	8340.0
57	8074.0
58	7808.0
59	6947.0
60	6086.0
61	5440.0
62	4794.0
63	4082.5
64	3371.0
65	2843.0
66	2315.0
67	2106.0
68	1897.0
69	1702.0
70	1507.0
71	1226.0
72	945.0
73	832.5
74	720.0
75	547.0
76	374.0
77	326.5
78	279.0
79	232.0
80	185.0
81	141.0
82	97.0
83	88.0
84	79.0
85	64.5
86	50.0
87	32.5
88	15.0
89	12.5
90	10.0
91	6.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034078807241746535
2	0.003407880724174654
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	8.519701810436635E-4
21	0.0
22	0.0
23	0.0
24	0.002981895633652822
25	0.0
26	0.0
27	0.0
28	4.2598509052183177E-4
29	0.0
30	8.519701810436635E-4
31	0.0
32	4.2598509052183177E-4
33	0.0
34	4.2598509052183177E-4
35	4.2598509052183177E-4
36	0.0
37	8.519701810436635E-4
38	0.0
39	0.0012779552715654952
40	0.0
41	0.001703940362087327
42	0.008093716719914802
43	0.006389776357827476
44	0.005111821086261981
45	0.001703940362087327
46	0.0021299254526091584
47	8.519701810436635E-4
48	0.0046858359957401485
49	0.004259850905218317
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	234750.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.88596611406903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.55524938225443	57.22445511062195
2	9.334164972589413	12.486492841194911
3	2.169671574001251	4.353617381319195
4	0.8354103497280906	2.2350891337302246
5	0.43675890762886405	1.4606520747841
6	0.279052842900516	1.1198831396567126
7	0.19597782319589283	0.917571622897265
8	0.1581480344730254	0.8462307259815518
9	0.12111038431675122	0.729052655532489
>10	0.8086739464446692	10.40935854832241
>50	0.07264532048944101	3.482675475863729
>100	0.030587503363975168	3.790835354587199
>500	0.0025489586136645975	0.9440859355082805
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	656	0.2794462193823216	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	522	0.2223642172523962	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	519	0.22108626198083067	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	518	0.22066027689030884	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	485	0.20660276890308837	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	475	0.20234291799787005	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	448	0.1908413205537806	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	329	0.14014909478168264	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	312	0.1329073482428115	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	289	0.12310969116080939	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	282	0.12012779552715655	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	240	0.10223642172523961	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	239	0.10181043663471778	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	237	0.10095846645367412	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.259850905218317E-4	0.0
11	0.0	0.0	0.0	4.259850905218317E-4	0.0
12	0.0	0.0	0.0	4.259850905218317E-4	0.0
13	0.0	0.0	0.0	4.259850905218317E-4	4.259850905218317E-4
14	0.0	0.0	0.0	4.259850905218317E-4	4.259850905218317E-4
15	0.0	0.0	0.0	4.259850905218317E-4	4.259850905218317E-4
16	0.0	0.0	0.0	4.259850905218317E-4	4.259850905218317E-4
17	0.0	0.0	0.0	8.519701810436634E-4	4.259850905218317E-4
18	0.0	0.0	0.0	8.519701810436634E-4	4.259850905218317E-4
19	0.0	0.0	0.0	8.519701810436634E-4	4.259850905218317E-4
20	0.0	0.0	0.0	0.0012779552715654952	4.259850905218317E-4
21	0.0	0.0	0.0	0.002129925452609159	8.519701810436634E-4
22	0.0	0.0	0.0	0.0038338658146964857	8.519701810436634E-4
23	0.0	0.0	0.0	0.006389776357827476	8.519701810436634E-4
24	0.0	0.0	0.0	0.00724174653887114	8.519701810436634E-4
25	0.0	0.0	0.0	0.009371671991480299	8.519701810436634E-4
26	0.0	0.0	0.0	0.013205537806176783	8.519701810436634E-4
27	0.0	0.0	0.0	0.016187433439829604	8.519701810436634E-4
28	0.0	0.0	0.0	0.020447284345047924	0.0012779552715654952
29	0.0	0.0	0.0	0.0268370607028754	0.0012779552715654952
30	0.0	0.0	0.0	0.03237486687965921	0.0012779552715654952
31	4.259850905218317E-4	0.0	0.0	0.04600638977635783	0.0012779552715654952
32	4.259850905218317E-4	0.0	0.0	0.06474973375931842	0.0012779552715654952
33	4.259850905218317E-4	0.0	0.0	0.07965921192758253	0.0012779552715654952
34	4.259850905218317E-4	0.0	0.0	0.09755058572949947	0.0017039403620873268
35	4.259850905218317E-4	0.0	0.0	0.11246006389776358	0.0017039403620873268
36	4.259850905218317E-4	0.0	0.0	0.1397231096911608	0.0017039403620873268
37	4.259850905218317E-4	0.0	0.0	0.18274760383386582	0.0017039403620873268
38	4.259850905218317E-4	0.0	0.0	0.23471778487752928	0.0017039403620873268
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGACC	30	1.2979427E-4	36.666664	4
GGCGTAG	25	0.0023489455	35.2	28
GTGCTAG	25	0.0023489455	35.2	1
GGTCTAC	25	0.0023489455	35.2	1
ATATGAG	35	3.21003E-4	31.42857	43
CAACGTA	30	0.00573571	29.333332	30
ATTCCGA	30	0.00573571	29.333332	6
AATTCCG	30	0.00573571	29.333332	5
GCGTAGG	30	0.00573571	29.333332	29
TAATCCT	30	0.00573571	29.333332	4
CGCCCCT	40	7.01515E-4	27.5	5
GGTCGTA	45	0.0013948638	24.444445	29
ATCGTAA	45	0.0013948638	24.444445	1
TATGAGA	45	0.0013948638	24.444445	44
CCCCCTT	45	0.0013948638	24.444445	28
ACTGTTC	135	0.0	24.444445	8
GGTTACC	45	0.0013948638	24.444445	11
GATATAC	145	0.0	24.275862	1
ATGCCGG	55	1.5882908E-4	24.0	19
GTAAACC	55	1.5882908E-4	24.0	33
>>END_MODULE