FastQCFastQC Report
Thu 2 Feb 2017
SRR4062047_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062047_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828020
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC30180.16509666196212297No Hit
GGGTAGGCACACGCTGAGCCAGTCA28840.15776632640780736No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC26060.1425586153324362No Hit
GAATAGGACCGCGGTTCTATTTTGT26020.1423397993457402No Hit
GTATCAACGCAGAGTACTTTTTTTT25690.1405345674554983No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24730.13528298377479459No Hit
TCGTAGTTCCGACCATAAACGATGC23830.13035962407413484No Hit
GATTAAGAGGGACGGCCGGGGGCAT23760.12997669609741686No Hit
ATCAGATACCGTCGTAGTTCCGACC23520.12866380017724097No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21790.11920000875263947No Hit
GTATCTGATCGTCTTCGAACCTCCG21650.11843415279920351No Hit
GAACTACGACGGTATCTGATCGTCT21070.11526132099211167No Hit
GAATAACGCCGCCGCATCGCCAGTC20930.11449546503867573No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT19750.10804039343114409No Hit
GTGCATGGCCGTTCTTAGTTGGTGG19180.10492226562072623No Hit
ACCATACTCCCCCCGGAACCCAAAG19080.10437522565398627No Hit
GCCATGCACCACCACCCACGGAATC18900.10339055371385433No Hit
GAGTATGGTTGCAAAGCTGAAACTT18870.10322644172383236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG402.7629983E-416.62377713
GGTATCA7850.015.2439461
TATACCG653.3687666E-614.6171135
TCGCGTA2700.013.3734189
GCGTTAT2550.013.0353991
GAGCGTT957.370181E-813.0026057
CGCATCG4050.012.66573613
GTATCAA21750.012.6192951
GTTTAGA1551.8189894E-1212.25447751
GTCCTAG1551.8189894E-1212.25447751
CGCGTAA2950.012.23974110
AAGACGG5500.012.0923395
CGTTATT2750.012.0890262
GTCGTAC550.003075531512.0873711
GTTCAAA5450.012.0240041
AACCGCG1353.7107384E-1011.9653627
TCGGCGT2000.011.87412613
GTATTAA1459.822543E-1111.7896521
ATCGCCA4750.011.59819416
CGGCGTC2050.011.58419714
Produced by FastQC (version 0.11.5)