##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062047_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1828020
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218143127536898	32.0	32.0	32.0	32.0	32.0
2	30.703984639117735	32.0	32.0	32.0	32.0	32.0
3	30.730867824203237	32.0	32.0	32.0	32.0	32.0
4	30.742320652946905	32.0	32.0	32.0	32.0	32.0
5	30.638455815581885	32.0	32.0	32.0	32.0	32.0
6	34.293591973829606	36.0	36.0	36.0	32.0	36.0
7	34.22392424590541	36.0	36.0	36.0	32.0	36.0
8	34.17246036695441	36.0	36.0	36.0	32.0	36.0
9	34.33120534786271	36.0	36.0	36.0	32.0	36.0
10	33.97816708788744	36.0	36.0	36.0	32.0	36.0
11	34.3174292403803	36.0	36.0	36.0	32.0	36.0
12	34.102028424196675	36.0	36.0	36.0	32.0	36.0
13	34.222553910788726	36.0	36.0	36.0	32.0	36.0
14	34.107173335083864	36.0	36.0	36.0	32.0	36.0
15	34.03623811555672	36.0	36.0	36.0	32.0	36.0
16	34.049751096815136	36.0	36.0	36.0	32.0	36.0
17	33.967208783273705	36.0	36.0	36.0	32.0	36.0
18	33.98777092154353	36.0	36.0	36.0	32.0	36.0
19	33.987741928425294	36.0	36.0	36.0	32.0	36.0
20	33.975566459885556	36.0	36.0	36.0	32.0	36.0
21	33.96109342348552	36.0	36.0	36.0	32.0	36.0
22	33.932279734357394	36.0	36.0	36.0	32.0	36.0
23	33.90111158521241	36.0	36.0	36.0	32.0	36.0
24	33.8676135928491	36.0	36.0	36.0	32.0	36.0
25	33.51057647071695	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	28.0
5	139.0
6	430.0
7	97.0
8	300.0
9	270.0
10	126.0
11	36.0
12	87.0
13	74.0
14	182.0
15	286.0
16	543.0
17	719.0
18	1072.0
19	1552.0
20	2479.0
21	3637.0
22	5653.0
23	8494.0
24	12231.0
25	17246.0
26	23676.0
27	30231.0
28	39815.0
29	52262.0
30	67912.0
31	91238.0
32	127649.0
33	178323.0
34	381346.0
35	779882.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.60044245028178	17.150418872627053	11.523018027332574	25.726120649758588
2	17.313049091366615	18.99880745287251	36.65824225117887	27.029901204582007
3	19.566216340409888	22.305044616872575	27.651191898503725	30.477547144213812
4	13.151656369353052	15.101954620332494	34.50109775399478	37.24529125631968
5	15.58976850985401	35.85196932435435	32.54006560219068	16.018196563600963
6	35.84284011904625	34.1808563688485	16.03746990042439	13.93883361168085
7	31.748109286265837	29.47990996862045	19.457456887345774	19.314523857767945
8	28.956952137278247	32.25577679032034	18.50122726331781	20.28604380908361
9	26.95436331889592	14.444258225929065	18.466085226737476	40.13529322843754
10	17.254402955315317	25.780568714148615	29.586840871344748	27.378187459191324
11	37.863806430054396	21.020821806747776	21.27576705432514	19.839604708872685
12	25.036645968176618	23.37744361231743	27.46541365574482	24.12049676376114
13	29.387641754158416	18.792668658966225	24.87993619967399	26.939753387201375
14	24.095722395195807	19.360536282008102	23.969209789118274	32.57453153367782
15	25.64514238323895	26.67720586382525	21.360717717135397	26.316934035800404
16	26.65040745696357	25.436115052986658	22.636412779454027	25.27706471059574
17	24.64507464386965	25.808879268415584	24.173319691346393	25.372726396368368
18	25.646002296872716	24.51399564393763	25.114994241388505	24.72500781780115
19	26.10998855540771	24.628379303358358	24.500517470799863	24.761114670434072
20	26.34680964301906	23.788709073347658	23.92789637718774	25.936584906445542
21	27.389384601232635	23.760354109448027	23.736043032795862	25.114218256523476
22	26.64007456303194	23.691012460393864	24.3922313381589	25.276681638415294
23	24.80496734445157	23.82631376372921	24.993361580606287	26.375357311212934
24	25.11442628067361	24.562595651828712	24.69855471626827	25.62442335122941
25	25.421754892389604	24.034457666272257	24.495977511516095	26.047809929822048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	279.0
1	279.0
2	380.5
3	482.0
4	482.0
5	482.0
6	1061.5
7	1641.0
8	1641.0
9	1641.0
10	1922.5
11	2204.0
12	2204.0
13	2204.0
14	3004.5
15	3805.0
16	3805.0
17	3805.0
18	6507.5
19	9210.0
20	9210.0
21	9210.0
22	14906.5
23	20603.0
24	20603.0
25	20603.0
26	31037.5
27	41472.0
28	41472.0
29	41472.0
30	51912.0
31	62352.0
32	62352.0
33	62352.0
34	80550.0
35	98748.0
36	98748.0
37	98748.0
38	117446.0
39	136144.0
40	136144.0
41	136144.0
42	159780.5
43	183417.0
44	183417.0
45	183417.0
46	206792.0
47	230167.0
48	230167.0
49	230167.0
50	240957.0
51	251747.0
52	251747.0
53	251747.0
54	237409.0
55	223071.0
56	223071.0
57	223071.0
58	209578.0
59	196085.0
60	196085.0
61	196085.0
62	175885.5
63	155686.0
64	155686.0
65	155686.0
66	128612.5
67	101539.0
68	101539.0
69	101539.0
70	76735.5
71	51932.0
72	51932.0
73	51932.0
74	40070.5
75	28209.0
76	28209.0
77	28209.0
78	22767.0
79	17325.0
80	17325.0
81	17325.0
82	11947.5
83	6570.0
84	6570.0
85	6570.0
86	4924.5
87	3279.0
88	3279.0
89	3279.0
90	2162.5
91	1046.0
92	1046.0
93	1046.0
94	647.5
95	249.0
96	249.0
97	249.0
98	503.5
99	758.0
100	758.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.846719401319461E-4
2	0.0
3	6.564479600879641E-4
4	0.01077668734477741
5	0.035119965864706074
6	0.0638395641185545
7	0.10886095338125404
8	0.10973621732803798
9	0.12078642465618537
10	0.13282130392446473
11	0.13840111158521243
12	0.13369656787124867
13	0.12713208827036904
14	0.11569895296550366
15	0.13424360783798864
16	0.11941882473933546
17	0.12494392840340916
18	0.11263552915175984
19	0.10010831391341453
20	0.09321561033249089
21	0.09255916237240293
22	0.10470344963403028
23	0.08375181890788941
24	0.0949114342293848
25	0.09097274646885702
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1828020.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.81159687386865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95025505529445	33.87779273312916
2	14.38624559003528	13.181137669983336
3	4.921044128622337	6.763226694568942
4	2.155478616536433	3.949836698040447
5	1.187749250196084	2.720634491861138
6	0.7219667790657917	1.9844670623332463
7	0.4672462639478412	1.498370823935981
8	0.35807357318266064	1.3123137748663805
9	0.24883302123472384	1.025949425194077
>10	1.3784243603560085	11.446220537571849
>50	0.11062917149446078	3.5241325056276134
>100	0.09239285074910594	8.869816028819374
>500	0.01483981254869362	4.697279597126322
>1k	0.006821526736093035	5.148821956942053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	3018	0.16509666196212297	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2884	0.15776632640780736	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2606	0.1425586153324362	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2602	0.1423397993457402	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2569	0.1405345674554983	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2473	0.13528298377479459	No Hit
TCGTAGTTCCGACCATAAACGATGC	2383	0.13035962407413484	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2376	0.12997669609741686	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2352	0.12866380017724097	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2179	0.11920000875263947	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2165	0.11843415279920351	No Hit
GAACTACGACGGTATCTGATCGTCT	2107	0.11526132099211167	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2093	0.11449546503867573	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1975	0.10804039343114409	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1918	0.10492226562072623	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1908	0.10437522565398627	No Hit
GCCATGCACCACCACCCACGGAATC	1890	0.10339055371385433	No Hit
GAGTATGGTTGCAAAGCTGAAACTT	1887	0.10322644172383236	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.4703996673997004E-5
7	0.0	0.0	0.0	0.0	5.4703996673997004E-5
8	0.0	0.0	0.0	0.0	5.4703996673997004E-5
9	0.0	0.0	0.0	0.0	5.4703996673997004E-5
10	0.0	0.0	0.0	5.4703996673997004E-5	1.0940799334799401E-4
11	0.0	0.0	0.0	5.4703996673997004E-5	1.0940799334799401E-4
12	0.0	0.0	0.0	5.4703996673997004E-5	3.8292797671797904E-4
13	0.0	0.0	0.0	5.4703996673997004E-5	3.8292797671797904E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	40	2.7629983E-4	16.623777	13
GGTATCA	785	0.0	15.243946	1
TATACCG	65	3.3687666E-6	14.617113	5
TCGCGTA	270	0.0	13.373418	9
GCGTTAT	255	0.0	13.035399	1
GAGCGTT	95	7.370181E-8	13.002605	7
CGCATCG	405	0.0	12.665736	13
GTATCAA	2175	0.0	12.619295	1
GTTTAGA	155	1.8189894E-12	12.2544775	1
GTCCTAG	155	1.8189894E-12	12.2544775	1
CGCGTAA	295	0.0	12.239741	10
AAGACGG	550	0.0	12.092339	5
CGTTATT	275	0.0	12.089026	2
GTCGTAC	55	0.0030755315	12.087371	1
GTTCAAA	545	0.0	12.024004	1
AACCGCG	135	3.7107384E-10	11.965362	7
TCGGCGT	200	0.0	11.874126	13
GTATTAA	145	9.822543E-11	11.789652	1
ATCGCCA	475	0.0	11.598194	16
CGGCGTC	205	0.0	11.584197	14
>>END_MODULE