FastQCFastQC Report
Thu 2 Feb 2017
SRR4062047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828020
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT29680.16236146212842312No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27120.14835723897987987No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC27090.1481931269898579No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA25510.13954989551536637No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA25070.1371429196617105No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24020.1313990000109408No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG23950.13101607203422283No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT23800.13019551208411287No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC21200.11597247294887364No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA20680.11312786512182581No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT19560.10700101749433813No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC19550.10694631349766413No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC18550.10147591383026444No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG18480.10109298585354645No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG18360.1004365378934585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22900.027.7771241
GGTATCA10300.026.4977741
CGTCGTA6900.019.76704610
ACCGTCG6850.019.5901838
GTATAGA1950.019.1884211
ATACCGT7200.018.9434196
TACCGTC7500.018.7723187
CAACGTA851.4313338E-418.11765930
CTTAGTA1102.7972692E-618.0005042
CCGTCGT7350.017.9582129
GCATATA2700.017.9342771
GTCGTAG7400.017.83687411
TCAACGC35400.017.7109074
CATATAG2500.017.6004922
AACGTAT750.001291444817.59953131
CAACGCA35950.017.4399475
AACGCAG36400.017.2847796
ATCAACG36300.017.2717933
CGCGGGA5100.017.25680244
TAAACGC5650.017.13275328
Produced by FastQC (version 0.11.5)