##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1828020
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30234242513758	32.0	32.0	32.0	32.0	32.0
2	31.448977035262196	32.0	32.0	32.0	32.0	32.0
3	31.573169330751305	32.0	32.0	32.0	32.0	32.0
4	31.650536099167404	32.0	32.0	32.0	32.0	32.0
5	31.622455990634677	32.0	32.0	32.0	32.0	32.0
6	35.262530497478146	36.0	36.0	36.0	36.0	36.0
7	35.27066990514327	36.0	36.0	36.0	36.0	36.0
8	35.21821971313224	36.0	36.0	36.0	36.0	36.0
9	35.30104594041641	36.0	36.0	36.0	36.0	36.0
10	35.182828415444035	36.0	36.0	36.0	36.0	36.0
11	35.30136267655715	36.0	36.0	36.0	36.0	36.0
12	35.21623122285314	36.0	36.0	36.0	36.0	36.0
13	35.26362184221179	36.0	36.0	36.0	36.0	36.0
14	35.22504403671732	36.0	36.0	36.0	36.0	36.0
15	35.19981783569108	36.0	36.0	36.0	36.0	36.0
16	35.2062351615409	36.0	36.0	36.0	36.0	36.0
17	35.18702749422873	36.0	36.0	36.0	36.0	36.0
18	35.188206365357054	36.0	36.0	36.0	36.0	36.0
19	35.17985415914487	36.0	36.0	36.0	36.0	36.0
20	35.176796752770755	36.0	36.0	36.0	36.0	36.0
21	35.16857528911062	36.0	36.0	36.0	36.0	36.0
22	35.15437960197372	36.0	36.0	36.0	36.0	36.0
23	35.12111136639643	36.0	36.0	36.0	36.0	36.0
24	35.1034507281102	36.0	36.0	36.0	36.0	36.0
25	35.087398387326175	36.0	36.0	36.0	36.0	36.0
26	35.048134593713414	36.0	36.0	36.0	36.0	36.0
27	35.0340970011269	36.0	36.0	36.0	36.0	36.0
28	35.015057275084516	36.0	36.0	36.0	36.0	36.0
29	34.99539009420028	36.0	36.0	36.0	36.0	36.0
30	34.97480333913196	36.0	36.0	36.0	36.0	36.0
31	34.98895362195162	36.0	36.0	36.0	36.0	36.0
32	34.954513626765575	36.0	36.0	36.0	36.0	36.0
33	34.93462489469481	36.0	36.0	36.0	32.0	36.0
34	34.92220763448977	36.0	36.0	36.0	32.0	36.0
35	34.91065360335226	36.0	36.0	36.0	32.0	36.0
36	34.88553079287973	36.0	36.0	36.0	32.0	36.0
37	34.87847014802902	36.0	36.0	36.0	32.0	36.0
38	34.86029310401418	36.0	36.0	36.0	32.0	36.0
39	34.850759291473835	36.0	36.0	36.0	32.0	36.0
40	34.83213695692607	36.0	36.0	36.0	32.0	36.0
41	34.81148072778197	36.0	36.0	36.0	32.0	36.0
42	34.77031761140469	36.0	36.0	36.0	32.0	36.0
43	34.77616492160917	36.0	36.0	36.0	32.0	36.0
44	34.71567652432687	36.0	36.0	36.0	32.0	36.0
45	34.715575321933024	36.0	36.0	36.0	32.0	36.0
46	34.69651918469163	36.0	36.0	36.0	32.0	36.0
47	34.68240555354974	36.0	36.0	36.0	32.0	36.0
48	34.65034955853875	36.0	36.0	36.0	32.0	36.0
49	34.636621590573405	36.0	36.0	36.0	32.0	36.0
50	34.21481603045918	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	9.0
21	44.0
22	119.0
23	315.0
24	808.0
25	1882.0
26	4038.0
27	7977.0
28	13721.0
29	22087.0
30	33946.0
31	49921.0
32	76860.0
33	131546.0
34	303358.0
35	1181384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.310491538640434	17.640723969519954	11.960053080153768	26.088731411685846
2	16.63941354267817	19.446312075167196	36.42364976681209	27.490624615342536
3	18.888377708473627	22.723908418594398	27.9675253950601	30.420188477871875
4	12.877102001072199	15.552236846424	34.62073719105918	36.949923961444625
5	15.2519202480937	36.18622125918822	32.34846027311533	16.21339821960275
6	35.98205049515404	34.30372912286921	15.878681653398191	13.835538728578564
7	31.767322020546825	29.41778536339865	19.501591886303213	19.313300729751315
8	28.984912637717315	32.33356309011937	18.428682399536108	20.252841872627215
9	27.116771151300313	14.196562400849006	18.356746643909805	40.329919803940875
10	17.18500583692283	25.77015105978169	29.634336204566914	27.41050689872857
11	38.086727716326955	20.951302502160807	21.26957035481012	19.692399426702114
12	25.00722092756097	23.27977812058949	27.53914070962025	24.1738602422293
13	29.473419328016103	18.666754193061344	24.953063970853712	26.906762508068837
14	24.23534753449087	19.18934147328804	23.956904191420225	32.61840680080086
15	25.64310018489951	26.679357993895035	21.34932878196081	26.32821303924465
16	26.815269306955607	25.281139514235996	22.617145339158988	25.286445839649414
17	24.804870843863853	25.7853305762519	24.081574599840263	25.328223980043983
18	25.817798293014622	24.357747024372845	25.179730177711594	24.644724504900935
19	26.23833437270927	24.471778208115886	24.4803667355937	24.809520683581145
20	26.480483233753716	23.643257342104945	24.052006052456814	25.824253371684524
21	27.50511482368902	23.68048489622652	23.7646743471078	25.049725932976664
22	26.728099254931564	23.528790713449524	24.532062012450627	25.21104801916828
23	24.900014715383158	23.662719041760507	25.13934483175503	26.297921411101306
24	25.283069237493695	24.468899856564395	24.67716047829374	25.570870427648167
25	25.495209555287868	23.78515700645619	24.731867480227177	25.98776595802877
26	25.137252928317753	25.115808867775485	24.882768822086508	24.86416938182025
27	25.811235581346754	24.129793316681383	24.488652712725393	25.57031838924647
28	25.24870144611993	24.017516459290096	25.14065957510825	25.593122519481724
29	25.006605547346865	24.314705233945784	25.207042197275836	25.471647021431515
30	25.13168799695842	24.413414697523788	25.21221338132763	25.242683924190164
31	25.83510073506042	24.249827544990108	24.11853745022601	25.796534269723466
32	25.766543946696135	24.09729706073818	23.972188335950023	26.163970656615664
33	25.10092909471252	23.989396154081803	24.63692877961681	26.27274597158887
34	26.0427744298712	24.101426251963886	24.72582176883252	25.129977549332388
35	26.51244821563085	23.76115487143691	24.97236069984185	24.754036213090387
36	24.91605662526299	24.731977475057683	24.439803109159758	25.912162790519567
37	26.340544905108786	24.24523574553006	24.32953608010556	25.0846832692556
38	25.29485454207284	23.861117493244056	24.86931215194582	25.97471581273728
39	26.311251883884346	23.89774645036529	23.986203538830583	25.80479812691979
40	26.268873572977085	24.246137485441892	24.530926647917774	24.95406229366325
41	24.990973741794313	24.856619256017506	25.02571115973742	25.126695842450765
42	26.624170230830924	24.646619815549712	24.321878771367206	24.407331182252157
43	26.007146858533343	23.558155208935105	24.657427831171333	25.777270101360223
44	25.306820457104216	24.4011024419812	24.268384883108983	26.0236922178056
45	25.476797574586552	24.701678412802785	24.37032851069789	25.45119550191277
46	25.374567280970865	24.495726420915812	24.354586712298193	25.775119585815133
47	25.615261235752218	24.12560366388695	24.909956433355728	25.349178667005106
48	26.66824584658358	24.826732415986623	23.491796561508966	25.013225175920823
49	25.388590832029962	24.995090130971782	23.93477724512309	25.68154179187517
50	25.764934354485774	24.96750547045952	23.926805251641138	25.340754923413567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	113.0
1	76.5
2	40.0
3	120.5
4	201.0
5	222.0
6	243.0
7	185.0
8	127.0
9	190.0
10	253.0
11	409.5
12	566.0
13	1103.0
14	1640.0
15	2107.0
16	2574.0
17	2611.5
18	2649.0
19	2610.0
20	2571.0
21	3093.0
22	3615.0
23	4214.0
24	4813.0
25	6468.5
26	8124.0
27	10658.0
28	13192.0
29	15216.5
30	17241.0
31	19650.0
32	22059.0
33	25351.5
34	28644.0
35	33639.0
36	38634.0
37	48096.0
38	57558.0
39	64280.5
40	71003.0
41	78877.0
42	86751.0
43	90020.0
44	93289.0
45	103962.0
46	114635.0
47	123794.0
48	132953.0
49	139953.5
50	146954.0
51	142246.5
52	137539.0
53	138214.0
54	138889.0
55	142502.5
56	146116.0
57	142651.0
58	139186.0
59	129046.5
60	118907.0
61	103752.5
62	88598.0
63	76443.0
64	64288.0
65	53872.5
66	43457.0
67	38118.0
68	32779.0
69	29674.0
70	26569.0
71	20489.0
72	14409.0
73	12679.0
74	10949.0
75	7850.5
76	4752.0
77	4408.0
78	4064.0
79	3519.5
80	2975.0
81	2336.0
82	1697.0
83	1481.0
84	1265.0
85	990.0
86	715.0
87	481.5
88	248.0
89	157.5
90	67.0
91	54.5
92	42.0
93	30.5
94	19.0
95	18.5
96	18.0
97	20.5
98	23.0
99	15.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03254887802102822
2	0.005196879684029715
3	3.8292797671797904E-4
4	0.0
5	5.4703996673997004E-5
6	1.6411199002199102E-4
7	0.0
8	0.0
9	0.0
10	1.0940799334799401E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.11151956761961E-4
17	0.0
18	1.0940799334799401E-4
19	0.0
20	1.0940799334799401E-4
21	0.0
22	0.0
23	5.4703996673997004E-5
24	0.001203487926827934
25	3.2822398004398205E-4
26	4.3763197339197603E-4
27	3.2822398004398205E-4
28	0.0013675999168499251
29	6.01743963413967E-4
30	0.0013675999168499251
31	3.8292797671797904E-4
32	5.4703996673997E-4
33	2.1881598669598801E-4
34	6.564479600879641E-4
35	3.8292797671797904E-4
36	1.0940799334799401E-4
37	0.0017505278935679041
38	0.0
39	8.20559950109955E-4
40	5.4703996673997004E-5
41	0.00109407993347994
42	0.005579807660747694
43	0.004595135720615749
44	0.004266911740571766
45	0.0023522718569818713
46	0.002406975853655868
47	8.752639467839521E-4
48	0.003665167777157799
49	0.003774575770505793
50	0.00109407993347994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1828020.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.43605778811686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.00361842534015	33.90000243940728
2	14.999063326027903	13.929947427501121
3	5.114459679456616	7.124860356907233
4	2.2855998708452954	4.245369907323384
5	1.2104282447485664	2.8103757960756646
6	0.7317887739678557	2.0388831477999916
7	0.48031667902624947	1.561280914470151
8	0.3462439258441021	1.2862562359424938
9	0.24721656573660328	1.0331786459452232
>10	1.3708527685616236	11.38883577944085
>50	0.10460446536794145	3.364054193209716
>100	0.08641975282171202	8.453423058199796
>500	0.013240259101288005	4.254811602871913
>1k	0.006147263154169431	4.6087204949051905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2968	0.16236146212842312	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2712	0.14835723897987987	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2709	0.1481931269898579	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2551	0.13954989551536637	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2507	0.1371429196617105	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	2402	0.1313990000109408	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2395	0.13101607203422283	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2380	0.13019551208411287	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2120	0.11597247294887364	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2068	0.11312786512182581	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	1956	0.10700101749433813	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1955	0.10694631349766413	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC	1855	0.10147591383026444	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1848	0.10109298585354645	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	1836	0.1004365378934585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0940799334799401E-4
2	0.0	0.0	0.0	0.0	1.0940799334799401E-4
3	0.0	0.0	0.0	0.0	1.0940799334799401E-4
4	0.0	0.0	0.0	0.0	1.0940799334799401E-4
5	0.0	0.0	0.0	0.0	1.0940799334799401E-4
6	0.0	0.0	0.0	0.0	2.1881598669598801E-4
7	0.0	0.0	0.0	5.4703996673997004E-5	2.1881598669598801E-4
8	0.0	0.0	0.0	5.4703996673997004E-5	2.1881598669598801E-4
9	0.0	0.0	0.0	5.4703996673997004E-5	2.1881598669598801E-4
10	0.0	0.0	0.0	5.4703996673997004E-5	2.1881598669598801E-4
11	0.0	0.0	0.0	5.4703996673997004E-5	2.1881598669598801E-4
12	0.0	0.0	0.0	5.4703996673997004E-5	6.01743963413967E-4
13	0.0	0.0	0.0	5.4703996673997004E-5	6.56447960087964E-4
14	0.0	0.0	0.0	5.4703996673997004E-5	6.56447960087964E-4
15	0.0	0.0	0.0	5.4703996673997004E-5	7.11151956761961E-4
16	0.0	0.0	0.0	5.4703996673997004E-5	7.658559534359581E-4
17	0.0	0.0	0.0	5.4703996673997004E-5	7.658559534359581E-4
18	0.0	0.0	0.0	5.4703996673997004E-5	7.658559534359581E-4
19	0.0	0.0	0.0	1.0940799334799401E-4	7.658559534359581E-4
20	0.0	0.0	0.0	2.1881598669598801E-4	9.846719401319461E-4
21	0.0	0.0	0.0	2.1881598669598801E-4	9.846719401319461E-4
22	0.0	0.0	0.0	2.73519983369985E-4	9.846719401319461E-4
23	0.0	0.0	0.0	4.3763197339197603E-4	9.846719401319461E-4
24	0.0	0.0	0.0	5.4703996673997E-4	9.846719401319461E-4
25	0.0	0.0	0.0	7.11151956761961E-4	9.846719401319461E-4
26	0.0	0.0	0.0	9.846719401319461E-4	9.846719401319461E-4
27	0.0	0.0	0.0	0.001258191923501931	9.846719401319461E-4
28	0.0	0.0	0.0	0.0013675999168499251	9.846719401319461E-4
29	0.0	0.0	0.0	0.0019146398835898951	9.846719401319461E-4
30	0.0	0.0	0.0	0.002844607827047844	9.846719401319461E-4
31	0.0	0.0	0.0	0.004102799750549775	0.001039375936805943
32	0.0	0.0	0.0	0.006345663614183652	0.00109407993347994
33	0.0	0.0	0.0	0.008369711491121541	0.001148783930153937
34	0.0	0.0	0.0	0.01077668734477741	0.001148783930153937
35	0.0	0.0	0.0	0.012910143215063292	0.001148783930153937
36	0.0	0.0	0.0	0.01712235095896106	0.001148783930153937
37	0.0	0.0	0.0	0.022483342633012767	0.001148783930153937
38	0.0	0.0	0.0	0.029102526230566406	0.001148783930153937
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2290	0.0	27.777124	1
GGTATCA	1030	0.0	26.497774	1
CGTCGTA	690	0.0	19.767046	10
ACCGTCG	685	0.0	19.590183	8
GTATAGA	195	0.0	19.188421	1
ATACCGT	720	0.0	18.943419	6
TACCGTC	750	0.0	18.772318	7
CAACGTA	85	1.4313338E-4	18.117659	30
CTTAGTA	110	2.7972692E-6	18.000504	2
CCGTCGT	735	0.0	17.958212	9
GCATATA	270	0.0	17.934277	1
GTCGTAG	740	0.0	17.836874	11
TCAACGC	3540	0.0	17.710907	4
CATATAG	250	0.0	17.600492	2
AACGTAT	75	0.0012914448	17.599531	31
CAACGCA	3595	0.0	17.439947	5
AACGCAG	3640	0.0	17.284779	6
ATCAACG	3630	0.0	17.271793	3
CGCGGGA	510	0.0	17.256802	44
TAAACGC	565	0.0	17.132753	28
>>END_MODULE