FastQCFastQC Report
Thu 2 Feb 2017
SRR4062046_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062046_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054760
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA75480.7156130304524252No Hit
GTCCTACAGTGGACATTTCTAAATT75460.7154234138571808No Hit
GTCCTAAAGTGTGTATTTCTCATTT72810.6902992149872957No Hit
CTTTAGGACGTGAAATATGGCGAGG60400.5726421176381358No Hit
GTCCTACAGTGTGCATTTCTCATTT46650.44228070840759987No Hit
CTGTAGGACCTGGAATATGGCGAGA36720.3481360688687474No Hit
GTATCAACGCAGAGTACTTTTTTTT32520.3083165838674201No Hit
CTGAAGGACCTGGAATATGGCGAGA26150.24792369828207364No Hit
ATTTAGAAATGTCCACTGTAGGACG26110.2475444650915848No Hit
GTCCTTCAGTGTGCATTTCTCATTT25060.23758959384125297No Hit
GAATATGGCAAGAAAACTGAAAATC21420.20307937350676933No Hit
TATCAACGCAGAGTACTTTTTTTTT21060.19966627479236984No Hit
TTTCTAAATTTTCCACCTTTTTCAG20080.19037506162539344No Hit
CTGTAGGACATGGAATATGGCAAGA17580.1666729872198415No Hit
GATATACACTGTTCTACAAATCCCG16610.1574765823504873No Hit
ATTCCAGGTCCTTCAGTGTGCATTT16130.15292578406462135No Hit
GGAATATGGCGAGAAAACTGAAAAT15710.1489438355644886No Hit
TTGTAGAACAGTGTATATCAATGAG14870.14097993856422314No Hit
GAAATATGGCGAGGAAAACTGAAAA14410.1366187568736016No Hit
GGTATCAACGCAGAGTACTTTTTTT14270.13529144070689067No Hit
ACCTGGAATATGGCGAGAAAACTGA13800.13083545071864688No Hit
TTCCAGGTCCTTCAGTGTGCATTTC13590.12884447646858052No Hit
GTGTATATCAATGAGTTACAATGAG13210.12524176115893662No Hit
GCCATATTCCACGTCCTACAGTGGA13030.12353521180173688No Hit
GACCTGGAATATGGCGAGAAAACTG12980.12306117031362586No Hit
CCATATTTCACGTCCTAAAGTGTGT12640.11983768819447077No Hit
GTCCACTGTAGGACGTGGAATATGG12520.1186999886230043No Hit
CCATATTCCAGGTCCTTCAGTGTGC12190.11557131480147143No Hit
ACAGTGGACATTTCTAAATTTTCCA12010.11386476544427168No Hit
GTGTATATCAATGAGTTACAATGAA11910.1129166824680496No Hit
ATACACACTTTAGGACGTGAAATAT11560.10959839205127234No Hit
CCACTGTAGGACGTGGAATATGGCA11190.10609048503925063No Hit
CCTAAAGTGTGTATTTCTCATTTTC10750.10191891994387348No Hit
GTTCTACAGTGTGGTTTTTATCATT10660.10106564526527362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTT307.724886E-418.99838616
GGCGATG359.945873E-518.99748419
TGGCGAC453.539718E-516.88745518
CGGGAGT350.002170119716.2851034
AGCCCCG508.716009E-515.19870916
GGCGACA508.719538E-515.19798819
GACGTCA456.754396E-414.7793287
GGCGATA456.766559E-414.77582319
GGTATCA9600.014.5428791
GCGAGCA400.00527061814.2528489
CCGTCGT602.5618807E-514.2528479
TGCAAAC400.005272277614.252171512
CCAACGA2200.014.24811419
TAGGACC14150.013.5613294
CACCTTT6600.013.24192714
TTGCGAG655.453582E-513.15272918
CCACCTT6900.013.0792213
TGGCGAT957.3860065E-812.99889718
TCCAACG3050.012.76940818
GGTCCAA604.0848297E-412.66859711
Produced by FastQC (version 0.11.5)