##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062046_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1054760
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39827069665137	32.0	32.0	32.0	32.0	32.0
2	31.135330312108916	32.0	32.0	32.0	32.0	32.0
3	31.152259281732338	32.0	32.0	32.0	32.0	32.0
4	31.142422920854035	32.0	32.0	32.0	32.0	32.0
5	31.176898062118397	32.0	32.0	32.0	32.0	32.0
6	34.85992358451212	36.0	36.0	36.0	36.0	36.0
7	34.77577553187455	36.0	36.0	36.0	36.0	36.0
8	34.763819257461414	36.0	36.0	36.0	36.0	36.0
9	34.784048503925064	36.0	36.0	36.0	36.0	36.0
10	34.621018999582844	36.0	36.0	36.0	32.0	36.0
11	34.775434222003106	36.0	36.0	36.0	36.0	36.0
12	34.67004531836626	36.0	36.0	36.0	32.0	36.0
13	34.6931538928287	36.0	36.0	36.0	32.0	36.0
14	34.68304448405324	36.0	36.0	36.0	32.0	36.0
15	34.65857161818803	36.0	36.0	36.0	32.0	36.0
16	34.65277788312033	36.0	36.0	36.0	32.0	36.0
17	34.61517596420039	36.0	36.0	36.0	32.0	36.0
18	34.60995013083545	36.0	36.0	36.0	32.0	36.0
19	34.52400830520687	36.0	36.0	36.0	32.0	36.0
20	34.50117751905647	36.0	36.0	36.0	32.0	36.0
21	34.45219955250484	36.0	36.0	36.0	32.0	36.0
22	34.38730422086541	36.0	36.0	36.0	32.0	36.0
23	34.42217850506276	36.0	36.0	36.0	32.0	36.0
24	34.346741438810724	36.0	36.0	36.0	32.0	36.0
25	34.02247146270241	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	11.0
5	91.0
6	198.0
7	33.0
8	194.0
9	159.0
10	90.0
11	17.0
12	40.0
13	41.0
14	163.0
15	216.0
16	378.0
17	485.0
18	602.0
19	739.0
20	1075.0
21	1436.0
22	2054.0
23	2984.0
24	3964.0
25	5715.0
26	8118.0
27	10654.0
28	14634.0
29	19951.0
30	27002.0
31	38332.0
32	55248.0
33	83178.0
34	208033.0
35	568925.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.327077214212984	21.192145277477024	13.031948681872741	24.448828826437254
2	11.782691591159686	20.95047304644947	47.87918377563026	19.387651586760576
3	18.492670795911458	27.127488615818113	30.072253883136618	24.307586705133808
4	10.381149421799797	17.606471139295238	40.60732829407236	31.40505114483261
5	10.387815837741767	41.19997951476549	36.50345498654234	11.9087496609504
6	28.644191445097235	40.14385609078378	19.318874993003572	11.893077471115417
7	26.400139707885238	32.66459572505261	22.87042758015882	18.064836986903334
8	22.563441586853543	42.88116916459363	20.34392330347084	14.211465945081988
9	26.63016600901569	15.070949418935223	21.986583730186226	36.31230084186286
10	15.568232413707337	30.049754927788424	34.493522176115924	19.888490482388313
11	32.83750861608982	22.0290867799275	28.209315440982397	16.924089163000282
12	24.935681641689307	25.219631766718503	33.74971756883251	16.09496902275968
13	29.91007308638319	24.100849525968496	26.556491246056858	19.43258614159146
14	18.478782920027566	26.307451653381275	28.095996537381662	27.117768889209504
15	19.887593513841946	39.15818554665249	22.791079633919416	18.163141305586148
16	17.858844702974338	29.165203260058586	34.284911502660705	18.691040534306364
17	17.83716771148567	32.93030911707415	30.77188139564024	18.460641775799942
18	18.147614161741355	28.074330064134262	37.17784563166602	16.600210142458362
19	23.56052791161362	26.580826145423387	28.307727639819042	21.550918303143956
20	23.89625153489502	29.07883524479559	30.261012812459075	16.76390040785031
21	21.15600869630407	27.761176183666247	27.24550926615682	23.837305853872863
22	22.402566280554066	33.1066325634808	27.895583510888184	16.595217645076946
23	18.406839488366796	32.52704292708847	30.824477170076293	18.241640414468442
24	22.672543865476044	27.980242742861485	31.979047058711892	17.368166332950587
25	19.8279191540766	30.664438554687905	31.493812434345188	18.013829856890304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	888.0
1	888.0
2	720.0
3	552.0
4	552.0
5	552.0
6	1185.5
7	1819.0
8	1819.0
9	1819.0
10	2379.0
11	2939.0
12	2939.0
13	2939.0
14	4247.0
15	5555.0
16	5555.0
17	5555.0
18	10101.0
19	14647.0
20	14647.0
21	14647.0
22	25638.0
23	36629.0
24	36629.0
25	36629.0
26	57311.0
27	77993.0
28	77993.0
29	77993.0
30	104789.5
31	131586.0
32	131586.0
33	131586.0
34	140357.0
35	149128.0
36	149128.0
37	149128.0
38	148943.0
39	148758.0
40	148758.0
41	148758.0
42	144787.0
43	140816.0
44	140816.0
45	140816.0
46	134961.0
47	129106.0
48	129106.0
49	129106.0
50	110550.5
51	91995.0
52	91995.0
53	91995.0
54	69065.5
55	46136.0
56	46136.0
57	46136.0
58	37122.0
59	28108.0
60	28108.0
61	28108.0
62	23498.0
63	18888.0
64	18888.0
65	18888.0
66	15479.5
67	12071.0
68	12071.0
69	12071.0
70	9505.0
71	6939.0
72	6939.0
73	6939.0
74	5671.5
75	4404.0
76	4404.0
77	4404.0
78	3697.5
79	2991.0
80	2991.0
81	2991.0
82	2155.5
83	1320.0
84	1320.0
85	1320.0
86	1040.0
87	760.0
88	760.0
89	760.0
90	518.5
91	277.0
92	277.0
93	277.0
94	168.5
95	60.0
96	60.0
97	60.0
98	227.5
99	395.0
100	395.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.688497857332474E-4
2	9.48082976222079E-5
3	6.636580833554553E-4
4	0.010239296143198453
5	0.0324244377867951
6	0.061720201752057335
7	0.10741780120596155
8	0.10931396715840569
9	0.11974287989684858
10	0.13244719177822442
11	0.14202282983806744
12	0.1333004664568243
13	0.12789639349235846
14	0.11585573969433804
15	0.13652394857597938
16	0.12154423755167051
17	0.12400925328984792
18	0.11272706587280518
19	0.09698888846751867
20	0.08930941636011984
21	0.09281732337214153
22	0.10381508589631765
23	0.08343130190754294
24	0.09196404869354166
25	0.09035230763396412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1054760.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.75775081669208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41991433147378	40.31176221519978
2	12.904415628449046	13.09998225807687
3	3.5110291455760154	5.346358274438721
4	1.3185182507738518	2.6770008328015957
5	0.6390051992887372	1.6217233338034192
6	0.4045900270655124	1.2321647866025989
7	0.2619647121444112	0.9307717707254742
8	0.18540546936968325	0.7528611691454575
9	0.149070807641056	0.6809849017458983
>10	0.9941443337307873	9.850284785306334
>50	0.10889089078965325	3.8002370003489645
>100	0.08857138146095987	8.944788671367974
>500	0.007710035216841695	2.7122051405626855
>1k	0.006017588461925226	5.326669719311673
>5k	7.521985577406533E-4	2.7122051405626855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	7548	0.7156130304524252	No Hit
GTCCTACAGTGGACATTTCTAAATT	7546	0.7154234138571808	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7281	0.6902992149872957	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6040	0.5726421176381358	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4665	0.44228070840759987	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3672	0.3481360688687474	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3252	0.3083165838674201	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2615	0.24792369828207364	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2611	0.2475444650915848	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2506	0.23758959384125297	No Hit
GAATATGGCAAGAAAACTGAAAATC	2142	0.20307937350676933	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2106	0.19966627479236984	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2008	0.19037506162539344	No Hit
CTGTAGGACATGGAATATGGCAAGA	1758	0.1666729872198415	No Hit
GATATACACTGTTCTACAAATCCCG	1661	0.1574765823504873	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1613	0.15292578406462135	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1571	0.1489438355644886	No Hit
TTGTAGAACAGTGTATATCAATGAG	1487	0.14097993856422314	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1441	0.1366187568736016	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1427	0.13529144070689067	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1380	0.13083545071864688	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1359	0.12884447646858052	No Hit
GTGTATATCAATGAGTTACAATGAG	1321	0.12524176115893662	No Hit
GCCATATTCCACGTCCTACAGTGGA	1303	0.12353521180173688	No Hit
GACCTGGAATATGGCGAGAAAACTG	1298	0.12306117031362586	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1264	0.11983768819447077	No Hit
GTCCACTGTAGGACGTGGAATATGG	1252	0.1186999886230043	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1219	0.11557131480147143	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1201	0.11386476544427168	No Hit
GTGTATATCAATGAGTTACAATGAA	1191	0.1129166824680496	No Hit
ATACACACTTTAGGACGTGAAATAT	1156	0.10959839205127234	No Hit
CCACTGTAGGACGTGGAATATGGCA	1119	0.10609048503925063	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1075	0.10191891994387348	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1066	0.10106564526527362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.480829762220789E-5	0.0
4	0.0	0.0	0.0	9.480829762220789E-5	0.0
5	0.0	0.0	0.0	9.480829762220789E-5	0.0
6	0.0	0.0	0.0	9.480829762220789E-5	0.0
7	0.0	0.0	0.0	9.480829762220789E-5	0.0
8	0.0	0.0	0.0	1.8961659524441578E-4	0.0
9	0.0	0.0	0.0	1.8961659524441578E-4	0.0
10	0.0	0.0	0.0	1.8961659524441578E-4	0.0
11	0.0	0.0	0.0	1.8961659524441578E-4	0.0
12	0.0	0.0	0.0	2.844248928666237E-4	0.0
13	0.0	0.0	0.0	2.844248928666237E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCTT	30	7.724886E-4	18.998386	16
GGCGATG	35	9.945873E-5	18.997484	19
TGGCGAC	45	3.539718E-5	16.887455	18
CGGGAGT	35	0.0021701197	16.285103	4
AGCCCCG	50	8.716009E-5	15.198709	16
GGCGACA	50	8.719538E-5	15.197988	19
GACGTCA	45	6.754396E-4	14.779328	7
GGCGATA	45	6.766559E-4	14.775823	19
GGTATCA	960	0.0	14.542879	1
GCGAGCA	40	0.005270618	14.252848	9
CCGTCGT	60	2.5618807E-5	14.252847	9
TGCAAAC	40	0.0052722776	14.2521715	12
CCAACGA	220	0.0	14.248114	19
TAGGACC	1415	0.0	13.561329	4
CACCTTT	660	0.0	13.241927	14
TTGCGAG	65	5.453582E-5	13.152729	18
CCACCTT	690	0.0	13.07922	13
TGGCGAT	95	7.3860065E-8	12.998897	18
TCCAACG	305	0.0	12.769408	18
GGTCCAA	60	4.0848297E-4	12.668597	11
>>END_MODULE