FastQCFastQC Report
Thu 2 Feb 2017
SRR4062046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054760
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA36100.3422579544161705No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC28630.2714361560923812No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA27040.25636163677045015No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA24240.22981531343623193No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA15010.14230725473093406No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA11720.11111532481322765No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA11520.1092191588607835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG554.9621776E-627.9986114
TAGGACC15300.027.6064744
GGTATCA10250.026.6234131
GTATCAA25650.026.0826741
TAGCGAG959.622454E-1025.47362918
GGCGGGT450.001398499724.44323232
TGTAGGA45700.023.8762782
CTGTAGG44400.023.6924971
TGCGAGA852.352117E-723.29406519
GTCCTAC41300.023.2861271
GTAGGAC46400.023.231613
AGGACCT26400.023.082195
TTAGGAC27750.022.83133
CTCTAGG3000.022.7408311
GGACCTG25950.022.5499426
CCTACAC2150.022.5105113
TCCTACA45400.022.2896442
TAGGACG49150.022.1555634
CGGGTCA500.002580964521.9989134
TATAGCG1004.6249625E-821.9989116
Produced by FastQC (version 0.11.5)