##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062046_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1054760
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262511851037203	32.0	32.0	32.0	32.0	32.0
2	31.570663468466762	32.0	32.0	32.0	32.0	32.0
3	31.6677272554894	32.0	32.0	32.0	32.0	32.0
4	31.742418180439152	32.0	32.0	32.0	32.0	32.0
5	31.738189730365203	32.0	32.0	32.0	32.0	32.0
6	35.42516496643786	36.0	36.0	36.0	36.0	36.0
7	35.42888808828549	36.0	36.0	36.0	36.0	36.0
8	35.400746141302285	36.0	36.0	36.0	36.0	36.0
9	35.45147995752588	36.0	36.0	36.0	36.0	36.0
10	35.369571276878155	36.0	36.0	36.0	36.0	36.0
11	35.44731692517729	36.0	36.0	36.0	36.0	36.0
12	35.402249800902574	36.0	36.0	36.0	36.0	36.0
13	35.40747089385263	36.0	36.0	36.0	36.0	36.0
14	35.39620766809511	36.0	36.0	36.0	36.0	36.0
15	35.38266999127764	36.0	36.0	36.0	36.0	36.0
16	35.39203136258485	36.0	36.0	36.0	36.0	36.0
17	35.39290265084	36.0	36.0	36.0	36.0	36.0
18	35.37492510144488	36.0	36.0	36.0	36.0	36.0
19	35.33518430733058	36.0	36.0	36.0	36.0	36.0
20	35.32307254730934	36.0	36.0	36.0	36.0	36.0
21	35.27987030224885	36.0	36.0	36.0	36.0	36.0
22	35.22571675073002	36.0	36.0	36.0	36.0	36.0
23	35.24780803215897	36.0	36.0	36.0	36.0	36.0
24	35.223989343547345	36.0	36.0	36.0	36.0	36.0
25	35.16077117069286	36.0	36.0	36.0	36.0	36.0
26	35.15578709848686	36.0	36.0	36.0	36.0	36.0
27	35.0816745041526	36.0	36.0	36.0	36.0	36.0
28	35.01368747392772	36.0	36.0	36.0	36.0	36.0
29	34.98359532026243	36.0	36.0	36.0	36.0	36.0
30	34.90315806439379	36.0	36.0	36.0	36.0	36.0
31	34.90698452728583	36.0	36.0	36.0	36.0	36.0
32	34.84884713110091	36.0	36.0	36.0	36.0	36.0
33	34.90926845917555	36.0	36.0	36.0	36.0	36.0
34	34.87139254427547	36.0	36.0	36.0	36.0	36.0
35	34.85688403049035	36.0	36.0	36.0	36.0	36.0
36	34.75524574310744	36.0	36.0	36.0	32.0	36.0
37	34.63354033144981	36.0	36.0	36.0	32.0	36.0
38	34.604578292692175	36.0	36.0	36.0	32.0	36.0
39	34.36071807804619	36.0	36.0	36.0	32.0	36.0
40	34.491875876976756	36.0	36.0	36.0	32.0	36.0
41	34.475440858583944	36.0	36.0	36.0	32.0	36.0
42	34.46174674807539	36.0	36.0	36.0	32.0	36.0
43	34.489880162311806	36.0	36.0	36.0	32.0	36.0
44	34.33252114225037	36.0	36.0	36.0	32.0	36.0
45	34.305701771019	36.0	36.0	36.0	32.0	36.0
46	34.07431263984224	36.0	36.0	36.0	32.0	36.0
47	34.10770601843073	36.0	36.0	36.0	32.0	36.0
48	34.06643407030983	36.0	36.0	36.0	32.0	36.0
49	33.98706909628731	36.0	36.0	36.0	32.0	36.0
50	33.71137983996359	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	6.0
21	15.0
22	43.0
23	165.0
24	458.0
25	1209.0
26	2693.0
27	5556.0
28	9655.0
29	15154.0
30	22076.0
31	31540.0
32	47018.0
33	77593.0
34	167728.0
35	673847.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.08922669251408	21.740186059657074	13.416060235604752	24.754527012224095
2	11.49888452855806	21.418284250377592	47.35145456907153	19.73137665199282
3	18.093240787179557	27.50928416415976	30.46362513308803	23.93384991557265
4	10.330691342106261	17.91014069551367	40.76680951116842	30.99235845121165
5	10.209431529447457	41.59249497516023	36.26673366453032	11.931339830861996
6	28.893113125260722	40.26489438355645	19.110318935113202	11.731673556069627
7	26.427338920702343	32.52967499715575	23.042587887291894	18.00039819485001
8	22.63604990708787	42.885300921536654	20.24991467253214	14.22873449884334
9	26.481569266942245	14.924722211687966	21.94783647464826	36.64587204672153
10	15.5600473662704	30.15096339542967	34.34291624911472	19.946072989185208
11	32.758826652508624	21.979881679244567	28.34170806628996	16.919583601956845
12	24.882437710948462	25.207630171792633	33.728810345481435	16.181121771777466
13	29.95269065948652	24.052106640373168	26.62235958891122	19.372843111229095
14	18.57038568015473	26.221510106564526	28.175129887367746	27.032974325913006
15	19.963593613713073	39.093632674731694	22.798266904319465	18.144506807235768
16	18.041139372518384	29.08795967977428	34.106750755625	18.76415019208234
17	17.940763775645642	32.85742728203572	30.743391861655734	18.4584170806629
18	18.233092330183794	27.92270833688865	37.22010167261116	16.624097660316394
19	23.718002199552505	26.357275588759528	28.603378967727256	21.321343243960712
20	24.147787874909337	28.90311020094714	30.163023640561075	16.78607828358244
21	21.340399711782776	27.456482991391407	27.523512457810305	23.67960483901551
22	22.671413402100953	32.62192347074216	28.10288596457962	16.60377716257727
23	18.46195999461488	32.20880998295343	30.975162075092104	18.35406794733958
24	22.79032946195781	27.798530457454373	32.06437544441811	17.34676463616971
25	20.036690811179795	30.386628237703363	31.53826462892032	18.038416322196518
26	16.592431628866528	32.41776786713161	32.68503296490759	18.304767539094275
27	18.282297929951703	31.32434169733721	31.27409320431796	19.11926716839313
28	17.354349371889075	32.30756103342024	29.0734297226831	21.264659872007584
29	19.839413553238227	29.231511653372948	28.530593244523157	22.398481548865664
30	17.65703054855809	29.701151081728604	31.26048237160191	21.381335998111396
31	22.417464480003943	27.79187263808381	29.91899563691387	19.871667244998378
32	19.04988542384644	31.278578857499877	30.395152627572035	19.27638309108164
33	17.46797135648997	31.894679258484636	32.94041577270258	17.696933612322816
34	19.180925166224068	31.256950692627928	32.54322584192857	17.018898299219437
35	18.197978687094697	32.1102430884751	31.774052865106757	17.91772535932345
36	17.078498500605352	30.740007907019518	33.51040379840324	18.67108979397189
37	18.072434226119935	30.358568381132972	29.132590661294145	22.43640673145295
38	19.295289923774128	33.060601463840115	28.802381584436272	18.84172702794949
39	22.00113959681785	28.417107604675568	29.993865897328202	19.587886901178372
40	23.21904814275109	28.29006436541428	31.165697566932348	17.325189924902276
41	22.47392986591148	27.349488173037994	32.4493648263903	17.72721713466022
42	19.97855346842183	32.23995843430838	31.195317033210834	16.58617106405895
43	17.56208004535771	32.39852587446799	32.08261474296192	17.956779337212378
44	17.11879383873225	32.14072387121487	30.88455871616868	19.855923573884205
45	17.677342283406336	32.58509206060261	30.042190492443638	19.695375163547414
46	18.86531815746934	28.83085485117256	30.75473218645265	21.54909480490545
47	22.24726238445129	28.819530694477365	29.994595875799952	18.938611045271394
48	18.272630306477993	30.54549764156534	29.554338808694208	21.627533243262462
49	21.262888430633577	29.338927208514065	29.312000758491553	20.086183602360805
50	18.33605594516979	33.7147202125249	28.200412041939916	19.748811800365395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	409.0
1	227.5
2	46.0
3	114.5
4	183.0
5	210.5
6	238.0
7	206.0
8	174.0
9	258.5
10	343.0
11	553.5
12	764.0
13	1459.0
14	2154.0
15	2818.0
16	3482.0
17	3795.0
18	4108.0
19	4359.5
20	4611.0
21	5643.5
22	6676.0
23	8337.5
24	9999.0
25	13315.5
26	16632.0
27	23208.0
28	29784.0
29	38879.5
30	47975.0
31	59743.0
32	71511.0
33	86912.5
34	102314.0
35	118175.5
36	134037.0
37	131429.5
38	128822.0
39	115861.0
40	102900.0
41	89922.0
42	76944.0
43	67321.5
44	57699.0
45	53028.5
46	48358.0
47	44359.0
48	40360.0
49	37079.0
50	33798.0
51	29697.5
52	25597.0
53	22810.0
54	20023.0
55	19164.0
56	18305.0
57	17233.5
58	16162.0
59	14338.0
60	12514.0
61	10849.5
62	9185.0
63	8015.0
64	6845.0
65	5843.0
66	4841.0
67	4612.0
68	4383.0
69	4262.0
70	4141.0
71	3391.0
72	2641.0
73	2426.0
74	2211.0
75	1679.5
76	1148.0
77	1013.0
78	878.0
79	723.0
80	568.0
81	460.0
82	352.0
83	321.0
84	290.0
85	226.0
86	162.0
87	139.0
88	116.0
89	79.5
90	43.0
91	26.5
92	10.0
93	10.0
94	10.0
95	7.5
96	5.0
97	5.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0347946452273503
2	0.004645606583488187
3	6.636580833554553E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	9.48082976222079E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.792331904888316E-4
17	0.0
18	1.896165952444158E-4
19	0.0
20	4.740414881110395E-4
21	0.0
22	0.0
23	1.896165952444158E-4
24	9.48082976222079E-4
25	0.0
26	1.896165952444158E-4
27	1.896165952444158E-4
28	9.48082976222079E-4
29	9.48082976222079E-5
30	0.0012325078690887026
31	1.896165952444158E-4
32	2.844248928666237E-4
33	9.48082976222079E-5
34	8.532746785998711E-4
35	0.0
36	9.48082976222079E-5
37	9.48082976222079E-4
38	0.0
39	9.48082976222079E-5
40	9.48082976222079E-5
41	8.532746785998711E-4
42	0.0041715650953771475
43	0.0031286738215328606
44	0.003981948500132731
45	0.001896165952444158
46	0.0014221244643331183
47	9.48082976222079E-4
48	0.0033182904167772765
49	0.0033182904167772765
50	0.0012325078690887026
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1054760.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.20948334481488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.88546638525689	51.12728540316478
2	12.533029032298263	15.844125797542766
3	3.2585409316874134	6.179120662496794
4	1.2045109536180838	3.045460602454774
5	0.5706278109093555	1.8034544554881542
6	0.31702510342601764	1.2023395796937044
7	0.22004204496886984	0.9736120783633151
8	0.13754524815363295	0.6955331257860392
9	0.10786850282609552	0.613648109953458
>10	0.6563043433846544	7.883626526143426
>50	0.06337930554173922	2.824428509494142
>100	0.0406709649375802	4.607162021569119
>500	0.0036286349028422805	1.5293809548759731
>1k	0.0013607380885658552	1.6708221729736077
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3610	0.3422579544161705	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	2863	0.2714361560923812	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	2704	0.25636163677045015	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2424	0.22981531343623193	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1501	0.14230725473093406	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1172	0.11111532481322765	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1152	0.1092191588607835	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.480829762220789E-5	0.0
11	0.0	0.0	0.0	9.480829762220789E-5	0.0
12	0.0	0.0	0.0	1.8961659524441578E-4	0.0
13	0.0	0.0	0.0	1.8961659524441578E-4	0.0
14	0.0	0.0	0.0	2.844248928666237E-4	0.0
15	0.0	0.0	0.0	2.844248928666237E-4	0.0
16	0.0	0.0	0.0	2.844248928666237E-4	0.0
17	0.0	0.0	0.0	2.844248928666237E-4	0.0
18	0.0	0.0	0.0	2.844248928666237E-4	0.0
19	0.0	0.0	0.0	2.844248928666237E-4	0.0
20	0.0	0.0	0.0	4.7404148811103946E-4	0.0
21	0.0	0.0	0.0	6.636580833554553E-4	0.0
22	0.0	0.0	0.0	7.584663809776631E-4	0.0
23	0.0	0.0	0.0	8.532746785998711E-4	0.0
24	0.0	0.0	0.0	8.532746785998711E-4	0.0
25	0.0	0.0	0.0	0.0011376995714664948	0.0
26	0.0	0.0	0.0	0.001990974250066366	0.0
27	0.0	0.0	0.0	0.002844248928666237	0.0
28	0.0	0.0	0.0	0.0041715650953771475	0.0
29	0.0	0.0	0.0	0.007300238916910008	0.0
30	0.0	0.0	0.0	0.01346277826235352	0.0
31	0.0	0.0	0.0	0.024934582274640675	0.0
32	0.0	0.0	0.0	0.03678561947741666	0.0
33	0.0	0.0	0.0	0.049015889870681485	0.0
34	0.0	0.0	0.0	0.062383859835412794	0.0
35	0.0	0.0	0.0	0.07963897000265463	0.0
36	0.0	0.0	0.0	0.1032462361105844	0.0
37	0.0	0.0	0.0	0.13358489134969093	0.0
38	0.0	0.0	0.0	0.172835526565285	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	55	4.9621776E-6	27.998611	4
TAGGACC	1530	0.0	27.606474	4
GGTATCA	1025	0.0	26.623413	1
GTATCAA	2565	0.0	26.082674	1
TAGCGAG	95	9.622454E-10	25.473629	18
GGCGGGT	45	0.0013984997	24.443232	32
TGTAGGA	4570	0.0	23.876278	2
CTGTAGG	4440	0.0	23.692497	1
TGCGAGA	85	2.352117E-7	23.294065	19
GTCCTAC	4130	0.0	23.286127	1
GTAGGAC	4640	0.0	23.23161	3
AGGACCT	2640	0.0	23.08219	5
TTAGGAC	2775	0.0	22.8313	3
CTCTAGG	300	0.0	22.740831	1
GGACCTG	2595	0.0	22.549942	6
CCTACAC	215	0.0	22.510511	3
TCCTACA	4540	0.0	22.289644	2
TAGGACG	4915	0.0	22.155563	4
CGGGTCA	50	0.0025809645	21.99891	34
TATAGCG	100	4.6249625E-8	21.99891	16
>>END_MODULE