FastQCFastQC Report
Thu 2 Feb 2017
SRR4062045_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062045_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761747
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG36130.4743044606673869No Hit
TCCATGTACTCTGCGTTGATACCAC30890.40551521699461895No Hit
GTACATGGGAAGCAGTGGTATCAAC25490.33462553840054504No Hit
CCCATGTACTCTGCGTTGATACCAC23710.311258199901017No Hit
GTATCAACGCAGAGTACTTTTTTTT17580.230785286978485No Hit
GTCCTACAGTGGACATTTCTAAATT15220.19980387188922305No Hit
GAGTACATGGAAGCAGTGGTATCAA15070.1978347141504988No Hit
CATGTACTCTGCGTTGATACCACTG14640.1921897952994892No Hit
GTCCTAAAGTGTGTATTTCTCATTT14060.18457571870975534No Hit
GTCCTACAGTGTGCATTTCTCATTT12720.1669845762438185No Hit
CTGTAGGACGTGGAATATGGCAAGA12220.16042071711473757No Hit
CTTTAGGACGTGAAATATGGCGAGG11730.15398813516823828No Hit
GTATCAACGCAGAGTACATGGGAAG11430.1500498196907897No Hit
TATCAACGCAGAGTACTTTTTTTTT10910.14322340619654556No Hit
GCTTCCATGTACTCTGCGTTGATAC9850.12930802484289403No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9500.12471332345253738No Hit
CTGTAGGACCTGGAATATGGCGAGA9380.12313799726155798No Hit
GAGTACATGGGAAGCAGTGGTATCA8380.11001027900339613No Hit
GCGTTGATACCACTGCTTCCATGTA8170.10725345816918216No Hit
CATGGAAGCAGTGGTATCAACGCAG8130.10672834943885569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT350.002163719416.2914665
GAACCGG350.002163719416.2914666
CGCGTAT350.002163719416.2914667
ATACGCG350.00216752616.287184
AGTACTA400.005263476614.2550335
AGTACCC400.005263476614.2550335
ACGCGTA400.005263476614.2550336
TAATGGC400.00527267314.2512834
GTACTAG400.005298032414.240981
TAGGACC4550.013.9902714
TATAGGA500.001502505913.2950772
TAGATTA655.427336E-513.1584915
ATTTAGA1900.012.9917721
TTTAGAA2000.012.8202532
AGGACCT6100.012.7750025
TGTAGGA9400.012.7293282
ATAGATT604.084655E-412.6678074
GTAGGAC9500.012.4986593
AAATGTC2000.012.3543627
GGACCTG6050.012.2522586
Produced by FastQC (version 0.11.5)