##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062045_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	761747
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195146157451227	32.0	32.0	32.0	32.0	32.0
2	30.800865641741943	32.0	32.0	32.0	32.0	32.0
3	30.81631040227267	32.0	32.0	32.0	32.0	32.0
4	30.815235242147327	32.0	32.0	32.0	32.0	32.0
5	30.788932545845274	32.0	32.0	32.0	32.0	32.0
6	34.41793010015136	36.0	36.0	36.0	32.0	36.0
7	34.34170531685717	36.0	36.0	36.0	32.0	36.0
8	34.30539536092692	36.0	36.0	36.0	32.0	36.0
9	34.392636925383364	36.0	36.0	36.0	32.0	36.0
10	34.13168414184762	36.0	36.0	36.0	32.0	36.0
11	34.399240167667216	36.0	36.0	36.0	32.0	36.0
12	34.24474924088969	36.0	36.0	36.0	32.0	36.0
13	34.286239394444614	36.0	36.0	36.0	32.0	36.0
14	34.2101353861584	36.0	36.0	36.0	32.0	36.0
15	34.170987217540734	36.0	36.0	36.0	32.0	36.0
16	34.1686819902146	36.0	36.0	36.0	32.0	36.0
17	34.10978710779301	36.0	36.0	36.0	32.0	36.0
18	34.098004980656306	36.0	36.0	36.0	32.0	36.0
19	34.08897048495104	36.0	36.0	36.0	32.0	36.0
20	34.06505572060015	36.0	36.0	36.0	32.0	36.0
21	34.01122157356708	36.0	36.0	36.0	32.0	36.0
22	33.975385528265946	36.0	36.0	36.0	32.0	36.0
23	33.92037645044877	36.0	36.0	36.0	32.0	36.0
24	33.88362540318505	36.0	36.0	36.0	32.0	36.0
25	33.46997625195767	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	12.0
5	65.0
6	154.0
7	37.0
8	126.0
9	97.0
10	48.0
11	15.0
12	31.0
13	31.0
14	174.0
15	214.0
16	401.0
17	475.0
18	599.0
19	834.0
20	1237.0
21	1723.0
22	2596.0
23	3410.0
24	4918.0
25	6723.0
26	8992.0
27	11683.0
28	15096.0
29	19670.0
30	25980.0
31	35039.0
32	49207.0
33	69643.0
34	153958.0
35	348558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28865643740088	17.122598905657604	11.499653423233246	26.089091233708267
2	14.689561467420202	20.047653742394107	39.9816211461841	25.2811636440016
3	19.418309276726465	24.674530190549333	28.45834235866333	27.448818174060875
4	12.160989411355386	16.51316523668542	36.77482882894711	34.55101652301209
5	13.145367275783501	37.75451913038339	35.14711386304392	13.95299973078918
6	32.99789172254806	36.01988742717905	17.69481862902855	13.287402221244344
7	28.537860757780255	30.796141053154486	21.604530228022703	19.061467961042563
8	26.194105757833015	36.28023539121041	19.75619820591737	17.7694606450392
9	27.007959288815993	14.981232060057408	18.507055063138083	39.50375358798851
10	14.679457435159978	28.59546478047524	33.79804589376108	22.927031890603704
11	35.58089854405627	21.30791731028363	23.744306043974102	19.366878101686
12	24.144920525587974	23.895297785863388	31.223874397303707	20.73590729124493
13	30.268854941362967	20.961852409757327	25.002300264727612	23.766992384152097
14	22.082911128722134	21.307368899718355	25.85390471909758	30.755815252461925
15	24.17680934997877	29.592404128145507	23.1435898986367	23.087196623239027
16	23.45556991090193	26.26572753786241	27.448882626861902	22.829819924373755
17	22.687590035856616	27.036913386820327	27.63430458144499	22.641191995878067
18	23.023627759298858	25.692477727824087	29.63135754152501	21.65253697135205
19	24.67253957908458	26.328106931858798	25.975022601602088	23.024330887454532
20	24.18225680565739	26.21022313673859	27.70353902067911	21.903981036924915
21	23.739721599554304	26.064499344337257	25.349708696531952	24.846070359576487
22	23.650240679975138	27.78838559783644	25.410759176786442	23.150614545401982
23	22.437833626532726	27.627134454366658	26.11401536899111	23.821016550109505
24	23.525122098958956	27.2361158011886	26.48400986514914	22.754752234703304
25	23.805744723623373	26.268294701877988	26.441075260057733	23.484885314440906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	224.0
1	224.0
2	333.5
3	443.0
4	443.0
5	443.0
6	1139.5
7	1836.0
8	1836.0
9	1836.0
10	1883.5
11	1931.0
12	1931.0
13	1931.0
14	2327.5
15	2724.0
16	2724.0
17	2724.0
18	4710.0
19	6696.0
20	6696.0
21	6696.0
22	10818.0
23	14940.0
24	14940.0
25	14940.0
26	22633.0
27	30326.0
28	30326.0
29	30326.0
30	39941.0
31	49556.0
32	49556.0
33	49556.0
34	54550.5
35	59545.0
36	59545.0
37	59545.0
38	65034.5
39	70524.0
40	70524.0
41	70524.0
42	77415.0
43	84306.0
44	84306.0
45	84306.0
46	99349.5
47	114393.0
48	114393.0
49	114393.0
50	105285.0
51	96177.0
52	96177.0
53	96177.0
54	85692.0
55	75207.0
56	75207.0
57	75207.0
58	67554.0
59	59901.0
60	59901.0
61	59901.0
62	51113.5
63	42326.0
64	42326.0
65	42326.0
66	34032.5
67	25739.0
68	25739.0
69	25739.0
70	19605.5
71	13472.0
72	13472.0
73	13472.0
74	9753.0
75	6034.0
76	6034.0
77	6034.0
78	4495.0
79	2956.0
80	2956.0
81	2956.0
82	2139.0
83	1322.0
84	1322.0
85	1322.0
86	926.0
87	530.0
88	530.0
89	530.0
90	371.5
91	213.0
92	213.0
93	213.0
94	144.5
95	76.0
96	76.0
97	76.0
98	213.0
99	350.0
100	350.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014440490083978013
2	2.6255436516323664E-4
3	2.6255436516323664E-4
4	0.010764728971692701
5	0.034394621836384
6	0.060650058352707654
7	0.1068596266214373
8	0.10515302324787626
9	0.11486753475891602
10	0.12996441075580212
11	0.14072913972749482
12	0.1311459053990367
13	0.1266824811912617
14	0.11289837702019175
15	0.13364017186808744
16	0.11814946432345648
17	0.1232692744441396
18	0.10764728971692701
19	0.09701383792781593
20	0.09044997879873501
21	0.08992487006840853
22	0.10213364804849905
23	0.07929141827929746
24	0.08979359288582693
25	0.0874306035993578
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	761747.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.980238505242944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46526616428338	43.57009103407749
2	14.064968623808369	16.028505335067187
3	4.406378372504062	7.532294718288771
4	1.8632201211948882	4.246669075738096
5	0.9783568324844635	2.7873502829099372
6	0.5439507373028822	1.8596665647972583
7	0.34584352928931245	1.3794372749079997
8	0.2383071152033233	1.0863037009425414
9	0.16454738791984985	0.8438354468178998
>10	0.8129672361227507	8.298541088703816
>50	0.06642987601357038	2.6456704449997823
>100	0.0411999660000473	4.3192905817379215
>500	0.005323591110321274	2.0814364563707777
>1k	0.003240446762804254	3.3209079946404882
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3613	0.4743044606673869	No Hit
TCCATGTACTCTGCGTTGATACCAC	3089	0.40551521699461895	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2549	0.33462553840054504	No Hit
CCCATGTACTCTGCGTTGATACCAC	2371	0.311258199901017	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1758	0.230785286978485	No Hit
GTCCTACAGTGGACATTTCTAAATT	1522	0.19980387188922305	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1507	0.1978347141504988	No Hit
CATGTACTCTGCGTTGATACCACTG	1464	0.1921897952994892	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1406	0.18457571870975534	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1272	0.1669845762438185	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1222	0.16042071711473757	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1173	0.15398813516823828	No Hit
GTATCAACGCAGAGTACATGGGAAG	1143	0.1500498196907897	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1091	0.14322340619654556	No Hit
GCTTCCATGTACTCTGCGTTGATAC	985	0.12930802484289403	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	950	0.12471332345253738	No Hit
CTGTAGGACCTGGAATATGGCGAGA	938	0.12313799726155798	No Hit
GAGTACATGGGAAGCAGTGGTATCA	838	0.11001027900339613	No Hit
GCGTTGATACCACTGCTTCCATGTA	817	0.10725345816918216	No Hit
CATGGAAGCAGTGGTATCAACGCAG	813	0.10672834943885569	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.3127718258161832E-4
6	0.0	0.0	0.0	0.0	1.3127718258161832E-4
7	0.0	0.0	0.0	0.0	1.3127718258161832E-4
8	0.0	0.0	0.0	1.3127718258161832E-4	1.3127718258161832E-4
9	0.0	0.0	0.0	2.6255436516323664E-4	1.3127718258161832E-4
10	0.0	0.0	0.0	2.6255436516323664E-4	1.3127718258161832E-4
11	0.0	0.0	0.0	2.6255436516323664E-4	1.3127718258161832E-4
12	0.0	0.0	0.0	2.6255436516323664E-4	5.251087303264733E-4
13	0.0	0.0	0.0	2.6255436516323664E-4	9.189402780713282E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGT	35	0.0021637194	16.291466	5
GAACCGG	35	0.0021637194	16.291466	6
CGCGTAT	35	0.0021637194	16.291466	7
ATACGCG	35	0.002167526	16.28718	4
AGTACTA	40	0.0052634766	14.255033	5
AGTACCC	40	0.0052634766	14.255033	5
ACGCGTA	40	0.0052634766	14.255033	6
TAATGGC	40	0.005272673	14.251283	4
GTACTAG	40	0.0052980324	14.24098	1
TAGGACC	455	0.0	13.990271	4
TATAGGA	50	0.0015025059	13.295077	2
TAGATTA	65	5.427336E-5	13.158491	5
ATTTAGA	190	0.0	12.991772	1
TTTAGAA	200	0.0	12.820253	2
AGGACCT	610	0.0	12.775002	5
TGTAGGA	940	0.0	12.729328	2
ATAGATT	60	4.084655E-4	12.667807	4
GTAGGAC	950	0.0	12.498659	3
AAATGTC	200	0.0	12.354362	7
GGACCTG	605	0.0	12.252258	6
>>END_MODULE