FastQCFastQC Report
Thu 2 Feb 2017
SRR4062045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761747
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16140.21188137268673196No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13880.1822127294232862No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10650.1398101994494235No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9790.12852036174740433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGC454.0682946E-529.332599
TAGGACC5250.026.8183654
GTAATAC952.6828275E-823.1573093
GTATAAT1355.456968E-1222.8157311
GTCCTAC10200.022.0008871
TCGTATA602.870644E-422.00088744
TAGATTG500.002579949321.9994435
ATAGGAC1500.021.9994413
GTAGGAC12750.021.7406233
GTATTAA1158.754796E-921.0443231
TCCTACA11050.020.9044462
TCCTATA956.861119E-720.8415782
TAATACC856.1098835E-620.7053574
TGTAGGA13300.020.5107572
CTGTAGG13050.020.0621091
GTATTAC1001.1218854E-619.8007981
TAGGACT2000.019.7994984
ATCACGG6350.019.40108335
CTAGAAC1252.2851964E-819.3595093
CGGAAAA6600.018.99951739
Produced by FastQC (version 0.11.5)