##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062045_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	761747
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227993021304975	32.0	32.0	32.0	32.0	32.0
2	31.454439597399137	32.0	32.0	32.0	32.0	32.0
3	31.593724688118233	32.0	32.0	32.0	32.0	32.0
4	31.668122093030888	32.0	32.0	32.0	32.0	32.0
5	31.657476826295344	32.0	32.0	32.0	32.0	32.0
6	35.297594870737925	36.0	36.0	36.0	36.0	36.0
7	35.30457094022031	36.0	36.0	36.0	36.0	36.0
8	35.26628263714856	36.0	36.0	36.0	36.0	36.0
9	35.33537906942856	36.0	36.0	36.0	36.0	36.0
10	35.2380068447923	36.0	36.0	36.0	36.0	36.0
11	35.33626650318281	36.0	36.0	36.0	36.0	36.0
12	35.26496330146361	36.0	36.0	36.0	36.0	36.0
13	35.282901015691564	36.0	36.0	36.0	36.0	36.0
14	35.254471629031684	36.0	36.0	36.0	36.0	36.0
15	35.231726544377594	36.0	36.0	36.0	36.0	36.0
16	35.2461329023941	36.0	36.0	36.0	36.0	36.0
17	35.22809804305104	36.0	36.0	36.0	36.0	36.0
18	35.20977568667812	36.0	36.0	36.0	36.0	36.0
19	35.19219898470227	36.0	36.0	36.0	36.0	36.0
20	35.18052975594259	36.0	36.0	36.0	36.0	36.0
21	35.14697662084655	36.0	36.0	36.0	36.0	36.0
22	35.10111887542714	36.0	36.0	36.0	36.0	36.0
23	35.069305163000315	36.0	36.0	36.0	36.0	36.0
24	35.048605376850844	36.0	36.0	36.0	36.0	36.0
25	35.01316053755381	36.0	36.0	36.0	36.0	36.0
26	34.961739265136586	36.0	36.0	36.0	36.0	36.0
27	34.90746140122639	36.0	36.0	36.0	36.0	36.0
28	34.853131026443165	36.0	36.0	36.0	32.0	36.0
29	34.816737053116064	36.0	36.0	36.0	32.0	36.0
30	34.78355280690308	36.0	36.0	36.0	32.0	36.0
31	34.74625827210347	36.0	36.0	36.0	32.0	36.0
32	34.72642885367451	36.0	36.0	36.0	32.0	36.0
33	34.70318097741114	36.0	36.0	36.0	32.0	36.0
34	34.68388191879981	36.0	36.0	36.0	32.0	36.0
35	34.63063851908836	36.0	36.0	36.0	32.0	36.0
36	34.56664089257982	36.0	36.0	36.0	32.0	36.0
37	34.47739866386084	36.0	36.0	36.0	32.0	36.0
38	34.44157312073431	36.0	36.0	36.0	32.0	36.0
39	34.26573389852536	36.0	36.0	36.0	32.0	36.0
40	34.24762158564458	36.0	36.0	36.0	32.0	36.0
41	34.22817943490424	36.0	36.0	36.0	32.0	36.0
42	34.1526845527452	36.0	36.0	36.0	32.0	36.0
43	34.14452961416323	36.0	36.0	36.0	32.0	36.0
44	34.13093454913508	36.0	36.0	36.0	32.0	36.0
45	33.985241819134174	36.0	36.0	36.0	32.0	36.0
46	33.976509260948845	36.0	36.0	36.0	32.0	36.0
47	33.926352187799885	36.0	36.0	36.0	32.0	36.0
48	33.92746279276453	36.0	36.0	36.0	32.0	36.0
49	33.91511748651455	36.0	36.0	36.0	32.0	36.0
50	33.4937807434752	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	2.0
20	9.0
21	22.0
22	89.0
23	237.0
24	605.0
25	1425.0
26	3001.0
27	5706.0
28	9175.0
29	13647.0
30	19472.0
31	27222.0
32	39173.0
33	57627.0
34	119858.0
35	464474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8471114753065	17.67179341716831	11.917462468614412	26.56363263891078
2	14.165853812232596	20.572153248113807	39.78054899510187	25.481443944551728
3	18.90732749850016	25.063833530030312	28.662666213322797	27.366172758146735
4	11.994139786569557	16.827634372042162	36.7720516129371	34.40617422845118
5	12.781671604876685	38.11134142963478	34.98221850561932	14.124768459869221
6	33.13186661713141	36.12104806451486	17.5736826006535	13.173402717700233
7	28.527188160898564	30.805241110237386	21.636973955919746	19.030596772944296
8	26.420451934828755	36.244317338959	19.77717011028596	17.558060615926287
9	27.063710129478686	14.627822623521983	18.483433475944114	39.82503377105522
10	14.706851487436118	28.426761116223627	33.97295952593184	22.893427870408416
11	35.78904806976595	21.115147155157814	23.779942684382085	19.315862090694154
12	24.213945049996916	23.798058935578347	31.124901049823627	20.86309496460111
13	30.419680024995177	20.87162798146891	25.098490706231857	23.610201287304054
14	22.252795219410118	21.02246546425519	26.03200275156975	30.692736564764942
15	24.22326572996021	29.50060846974127	23.162283540335572	23.11384225996295
16	23.42266769063138	26.226886950357404	27.498047246782058	22.852398112229157
17	22.790244004899264	26.930988897888668	27.651963184626915	22.62680391258515
18	23.163889275427767	25.516116920863386	29.715679504716796	21.60431429899205
19	24.931243575622876	26.215659530001428	26.07466783590877	22.778429058466916
20	24.41522502251407	26.069319694491337	27.773037206627933	21.74241807636666
21	23.799502984586745	25.74660615663731	25.53735032760221	24.916540531173737
22	23.651553599817262	27.59367611556068	25.507944238703928	23.246826045918134
23	22.617220678256693	27.231351091635414	26.417957668359705	23.73347056174819
24	23.709270879827763	26.936487515425213	26.633365715335945	22.720875889411086
25	24.04249704954532	25.816576894953315	26.562362569199482	23.57856348630188
26	22.0398480852466	26.628666098671072	27.825920290701724	23.505565525380607
27	23.49859991414382	27.096945279157296	26.596511403512224	22.807943403186655
28	22.780231970435914	26.722154029944793	26.148069866817202	24.34954413280209
29	23.36358421038122	26.0320765194469	26.467132529298016	24.137206740873868
30	23.37626193008021	26.24131910256915	27.124834258858126	23.25758470849251
31	24.234881751767322	25.195308141175854	26.498237599196585	24.071572507860242
32	22.55183178578602	27.21967907811427	26.93611887473859	23.292370261361114
33	22.554296456552333	27.125779789535592	27.357222373920898	22.96270137999118
34	22.467305031388747	27.22484633824228	27.897387290643238	22.41046133972573
35	22.89424558842449	27.229942789328724	27.464404145213763	22.411407477033027
36	22.82275935222332	26.77553088701716	27.661655359280807	22.740054401478712
37	23.440890705798488	26.606041749536914	26.129234259248317	23.82383328541628
38	23.434552417009847	27.81290901047198	25.6438161226759	23.10872244984227
39	24.016073641538366	26.177167132264906	26.280351404146273	23.52640782205045
40	24.736099350832628	26.365253464085754	26.557706319043774	22.340940866037847
41	23.655740352010334	25.867127716973926	27.479228449639574	22.997903481376166
42	23.980029223605214	27.742469932901237	26.457077665734996	21.820423177758553
43	22.943631444141474	26.829316322685926	26.877496819693352	23.349555413479248
44	22.7907062230706	27.212313282938215	27.154154971551	22.84282552244018
45	23.622404906751246	27.114736795120553	26.36197267783954	22.900885620288662
46	23.132575673039433	26.019946674088356	27.14015840237564	23.707319250496568
47	24.81026816482941	26.035369348577518	26.669318514492613	22.48504397210046
48	22.86288178649495	27.67833113434815	25.890917206566762	23.56786987259014
49	23.52507945843546	26.384664160930736	27.07455111399348	23.015705266640325
50	22.724277177394793	28.575516977010405	25.239453038848104	23.460752806746694
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	54.0
2	54.0
3	136.5
4	219.0
5	239.0
6	259.0
7	226.0
8	193.0
9	262.0
10	331.0
11	454.5
12	578.0
13	996.0
14	1414.0
15	1873.0
16	2332.0
17	2412.0
18	2492.0
19	2513.0
20	2534.0
21	2973.5
22	3413.0
23	3896.0
24	4379.0
25	5783.5
26	7188.0
27	9854.5
28	12521.0
29	15160.5
30	17800.0
31	20071.5
32	22343.0
33	26669.0
34	30995.0
35	35014.5
36	39034.0
37	40971.5
38	42909.0
39	42053.0
40	41197.0
41	41509.0
42	41821.0
43	42107.0
44	42393.0
45	46627.5
46	50862.0
47	53504.0
48	56146.0
49	54580.5
50	53015.0
51	50892.5
52	48770.0
53	47049.5
54	45329.0
55	43487.0
56	41645.0
57	40196.0
58	38747.0
59	35491.0
60	32235.0
61	28379.0
62	24523.0
63	21015.0
64	17507.0
65	14712.0
66	11917.0
67	10111.5
68	8306.0
69	7070.5
70	5835.0
71	4773.5
72	3712.0
73	3151.0
74	2590.0
75	2093.5
76	1597.0
77	1357.0
78	1117.0
79	911.0
80	705.0
81	548.0
82	391.0
83	287.0
84	183.0
85	137.0
86	91.0
87	62.5
88	34.0
89	26.0
90	18.0
91	11.0
92	4.0
93	4.5
94	5.0
95	5.0
96	5.0
97	4.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03295057282798619
2	0.003675761112285313
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.6255436516323664E-4
17	0.0
18	1.3127718258161832E-4
19	0.0
20	1.3127718258161832E-4
21	0.0
22	0.0
23	0.0
24	9.189402780713283E-4
25	0.0
26	5.251087303264733E-4
27	0.0010502174606529466
28	0.0015753261909794195
29	7.876630954897098E-4
30	0.0022317121038875113
31	2.6255436516323664E-4
32	5.251087303264733E-4
33	3.938315477448549E-4
34	0.0011814946432345647
35	1.3127718258161832E-4
36	3.938315477448549E-4
37	0.0018378805561426565
38	0.0
39	3.938315477448549E-4
40	2.6255436516323664E-4
41	0.0010502174606529466
42	0.004463424207775023
43	0.003675761112285313
44	0.0043321470251934035
45	0.0027568208342139845
46	0.002100434921305893
47	0.0018378805561426565
48	0.004200869842611786
49	0.003675761112285313
50	0.0014440490083978013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	761747.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.053187731652294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8647498405176	47.69702751800129
2	13.960341514439541	17.325673855869923
3	4.362637643947602	8.121467181751614
4	1.8331246056951176	4.550049011708408
5	0.9500851409451193	2.9477905806060405
6	0.5277251680248789	1.9648217353299338
7	0.3300736820799783	1.4337486911570627
8	0.20876614929172319	1.036368404321076
9	0.15965857144680176	0.8916590976260229
>10	0.7268098895755081	7.792807202440041
>50	0.04926990354960838	2.1226088716665465
>100	0.024421884178119416	2.9102021788930146
>500	0.001698913678855688	0.6698314463177428
>1k	6.37092629570883E-4	0.5359442243112846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1614	0.21188137268673196	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1388	0.1822127294232862	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1065	0.1398101994494235	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	979	0.12852036174740433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.251087303264733E-4
2	0.0	0.0	0.0	0.0	5.251087303264733E-4
3	0.0	0.0	0.0	0.0	5.251087303264733E-4
4	0.0	0.0	0.0	0.0	5.251087303264733E-4
5	0.0	0.0	0.0	0.0	5.251087303264733E-4
6	0.0	0.0	0.0	0.0	5.251087303264733E-4
7	0.0	0.0	0.0	0.0	5.251087303264733E-4
8	0.0	0.0	0.0	0.0	5.251087303264733E-4
9	0.0	0.0	0.0	0.0	5.251087303264733E-4
10	0.0	0.0	0.0	0.0	5.251087303264733E-4
11	0.0	0.0	0.0	0.0	5.251087303264733E-4
12	0.0	0.0	0.0	0.0	0.0013127718258161831
13	0.0	0.0	0.0	0.0	0.001706603373561038
14	0.0	0.0	0.0	0.0	0.001706603373561038
15	0.0	0.0	0.0	1.3127718258161832E-4	0.0019691577387242745
16	0.0	0.0	0.0	1.3127718258161832E-4	0.0019691577387242745
17	0.0	0.0	0.0	1.3127718258161832E-4	0.0019691577387242745
18	0.0	0.0	0.0	2.6255436516323664E-4	0.002100434921305893
19	0.0	0.0	0.0	3.9383154774485493E-4	0.002100434921305893
20	0.0	0.0	0.0	3.9383154774485493E-4	0.0026255436516323663
21	0.0	0.0	0.0	3.9383154774485493E-4	0.0026255436516323663
22	0.0	0.0	0.0	9.189402780713282E-4	0.0027568208342139845
23	0.0	0.0	0.0	0.001706603373561038	0.0028880980167956026
24	0.0	0.0	0.0	0.0019691577387242745	0.0030193751993772213
25	0.0	0.0	0.0	0.0024942664690507477	0.0030193751993772213
26	0.0	0.0	0.0	0.003938315477448549	0.0031506523819588394
27	0.0	0.0	0.0	0.004594701390356641	0.0031506523819588394
28	0.0	0.0	0.0	0.005119810120683114	0.0031506523819588394
29	0.0	0.0	0.0	0.007088967859407389	0.0031506523819588394
30	0.0	0.0	0.0	0.009583234328458137	0.0031506523819588394
31	0.0	0.0	0.0	0.012208777980090502	0.0031506523819588394
32	0.0	0.0	0.0	0.014965598814304487	0.0031506523819588394
33	0.0	0.0	0.0	0.018510082744008182	0.0032819295645404576
34	0.0	0.0	0.0	0.02336733849952806	0.0035444839297036944
35	0.0	0.0	0.0	0.028093317072466317	0.0035444839297036944
36	0.0	0.0	0.0	0.03452589901896561	0.0035444839297036944
37	0.0	0.0	0.0	0.04279636152160757	0.0035444839297036944
38	0.0	0.0	0.0	0.05539897104944293	0.0036757611122853126
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTGC	45	4.0682946E-5	29.33259	9
TAGGACC	525	0.0	26.818365	4
GTAATAC	95	2.6828275E-8	23.157309	3
GTATAAT	135	5.456968E-12	22.815731	1
GTCCTAC	1020	0.0	22.000887	1
TCGTATA	60	2.870644E-4	22.000887	44
TAGATTG	50	0.0025799493	21.999443	5
ATAGGAC	150	0.0	21.999441	3
GTAGGAC	1275	0.0	21.740623	3
GTATTAA	115	8.754796E-9	21.044323	1
TCCTACA	1105	0.0	20.904446	2
TCCTATA	95	6.861119E-7	20.841578	2
TAATACC	85	6.1098835E-6	20.705357	4
TGTAGGA	1330	0.0	20.510757	2
CTGTAGG	1305	0.0	20.062109	1
GTATTAC	100	1.1218854E-6	19.800798	1
TAGGACT	200	0.0	19.799498	4
ATCACGG	635	0.0	19.401083	35
CTAGAAC	125	2.2851964E-8	19.359509	3
CGGAAAA	660	0.0	18.999517	39
>>END_MODULE