FastQCFastQC Report
Thu 2 Feb 2017
SRR4062044_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062044_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550917
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26050.16796514578149574No Hit
GGGTAGGCACACGCTGAGCCAGTCA17970.11586693549687056No Hit
GCGCAAGACGGACCAGAGCGAAAGC17640.11373916205702819No Hit
TATCAACGCAGAGTACTTTTTTTTT17140.11051526290575188No Hit
GAATAGGACCGCGGTTCTATTTTGT15580.1004566975537698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCC453.5426427E-516.8867933
GGTATCA7400.015.3995161
TCTTATA1251.03682396E-1012.9167282
CCGACCA2900.012.776349
CGTTATT1352.7284841E-1112.663462
GTATCAA19000.012.2453171
GTCTTAC701.0947962E-412.2096171
TCGCACG1650.012.09175113
TCGGAAT1353.7107384E-1011.96225219
CGACCAT3100.011.95283110
CGCCGGT2600.011.6931237
CGTCGTA2450.011.6338410
CCGTCGT2300.011.565659
ACGGTAT2300.011.565659
ACCGTCG2500.011.4000588
ACGCCCC1751.8189894E-1211.39932217
GCGTTAT1501.7826096E-1011.3956421
CGTTCGC600.005872595611.084105515
ACATCGT951.3585372E-511.0011226
GACCATA3400.010.89922611
Produced by FastQC (version 0.11.5)