##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062044_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1550917
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243115524557407	32.0	32.0	32.0	32.0	32.0
2	30.726626247568372	32.0	32.0	32.0	32.0	32.0
3	30.74235371718796	32.0	32.0	32.0	32.0	32.0
4	30.75341427039616	32.0	32.0	32.0	32.0	32.0
5	30.621529714356086	32.0	32.0	32.0	32.0	32.0
6	34.31345649057945	36.0	36.0	36.0	32.0	36.0
7	34.235545809350214	36.0	36.0	36.0	32.0	36.0
8	34.17825131841356	36.0	36.0	36.0	32.0	36.0
9	34.353361269494116	36.0	36.0	36.0	32.0	36.0
10	33.9585451703734	36.0	36.0	36.0	32.0	36.0
11	34.346121036780175	36.0	36.0	36.0	32.0	36.0
12	34.120869137419994	36.0	36.0	36.0	32.0	36.0
13	34.2388296730257	36.0	36.0	36.0	32.0	36.0
14	34.111023349411994	36.0	36.0	36.0	32.0	36.0
15	34.04457362966555	36.0	36.0	36.0	32.0	36.0
16	34.06681337557071	36.0	36.0	36.0	32.0	36.0
17	33.97819096702145	36.0	36.0	36.0	32.0	36.0
18	34.00629176158363	36.0	36.0	36.0	32.0	36.0
19	33.99416216341687	36.0	36.0	36.0	32.0	36.0
20	33.982235670896635	36.0	36.0	36.0	32.0	36.0
21	33.964934938491226	36.0	36.0	36.0	32.0	36.0
22	33.93773167745276	36.0	36.0	36.0	32.0	36.0
23	33.89633358845122	36.0	36.0	36.0	32.0	36.0
24	33.859544385676344	36.0	36.0	36.0	32.0	36.0
25	33.48190135255465	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	28.0
5	114.0
6	306.0
7	71.0
8	277.0
9	199.0
10	123.0
11	31.0
12	63.0
13	64.0
14	195.0
15	313.0
16	473.0
17	636.0
18	973.0
19	1349.0
20	2028.0
21	3114.0
22	4767.0
23	6998.0
24	10083.0
25	14034.0
26	19239.0
27	25191.0
28	32682.0
29	43611.0
30	57552.0
31	77888.0
32	110594.0
33	155563.0
34	330216.0
35	652142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49251175287591	17.2264824807837	11.740173589864446	25.540832176475938
2	16.9561522510626	19.114495213139108	37.48297775760327	26.446374778195025
3	19.684970829915972	22.681674687794988	27.920167850492355	29.713186631796678
4	12.969259727605758	15.370200681340265	35.0316327339955	36.628906857058475
5	15.07370962998127	36.28367500683691	33.1821043235071	15.460511039674717
6	35.22562971345546	34.6797414886019	16.34703290620155	13.747595891741094
7	31.03566239521739	29.83710487265367	19.728789972625975	19.398442759502966
8	28.390540251823616	33.131064592815015	18.680156126251322	19.798239029110047
9	26.95559441665424	14.50216156792268	18.36141725523156	40.180826760191515
10	16.66820540400248	26.561683890840467	30.47997629268463	26.290134412472423
11	37.53445496109471	21.165951363702987	21.65853866413217	19.641055011070137
12	24.789184338208948	23.426057145070743	28.301175376808178	23.483583139912128
13	29.612437432897533	19.37128495219625	24.859242177054327	26.157035437851896
14	23.67286971026355	19.544945169294806	24.61998073760015	32.16220438284149
15	25.349375498358445	27.23982864540122	21.774430459022426	25.636365397217908
16	26.054993754377588	25.688971231404345	23.38683803664744	24.86919697757063
17	24.168847073284258	26.099806135867574	24.691240866016837	25.04010592483133
18	24.84792504176252	25.186156273156573	25.765667722671044	24.200250962409857
19	25.828520238876635	24.862028928262788	24.800780163403186	24.50867066945739
20	25.97064994388523	24.397177675680524	24.55509928022082	25.077073100213422
21	26.80583360116063	24.160538907238823	24.235272402946485	24.798355088654063
22	26.178614004276657	24.151364999699872	24.78143933452997	24.8885816614935
23	24.757756440039234	24.233712766506635	25.22114242940478	25.787388364049352
24	24.87413722446846	24.928477158834596	25.097498878674156	25.099886738022786
25	25.122066436666785	24.498085270327305	25.039656265044535	25.34019202796138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	224.0
1	224.0
2	322.5
3	421.0
4	421.0
5	421.0
6	888.0
7	1355.0
8	1355.0
9	1355.0
10	1734.0
11	2113.0
12	2113.0
13	2113.0
14	2943.5
15	3774.0
16	3774.0
17	3774.0
18	6213.5
19	8653.0
20	8653.0
21	8653.0
22	14131.5
23	19610.0
24	19610.0
25	19610.0
26	29547.0
27	39484.0
28	39484.0
29	39484.0
30	51111.5
31	62739.0
32	62739.0
33	62739.0
34	77044.0
35	91349.0
36	91349.0
37	91349.0
38	107109.5
39	122870.0
40	122870.0
41	122870.0
42	141723.0
43	160576.0
44	160576.0
45	160576.0
46	179250.5
47	197925.0
48	197925.0
49	197925.0
50	204657.5
51	211390.0
52	211390.0
53	211390.0
54	199314.5
55	187239.0
56	187239.0
57	187239.0
58	173683.0
59	160127.0
60	160127.0
61	160127.0
62	140919.5
63	121712.0
64	121712.0
65	121712.0
66	100221.5
67	78731.0
68	78731.0
69	78731.0
70	59477.0
71	40223.0
72	40223.0
73	40223.0
74	30305.5
75	20388.0
76	20388.0
77	20388.0
78	16084.0
79	11780.0
80	11780.0
81	11780.0
82	8138.0
83	4496.0
84	4496.0
85	4496.0
86	3339.0
87	2182.0
88	2182.0
89	2182.0
90	1462.0
91	742.0
92	742.0
93	742.0
94	471.5
95	201.0
96	201.0
97	201.0
98	407.0
99	613.0
100	613.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001289559660510524
2	3.22389915127631E-4
3	8.382137793318404E-4
4	0.00967169745382893
5	0.032819293359992834
6	0.062285731602658295
7	0.1056149361958119
8	0.10690449585632242
9	0.1167051492762024
10	0.1307613495757671
11	0.13901453140303446
12	0.13230882116837975
13	0.12766640639054186
14	0.11509319970056425
15	0.13424316065914552
16	0.11812366490276398
17	0.1221212998503466
18	0.10838748946590951
19	0.09658801857223823
20	0.09117186799809403
21	0.09097843404901745
22	0.10161730124822928
23	0.0797592650025759
24	0.09104291203204298
25	0.08710975506748589
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1550917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.51785255981237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20254944533005	36.24833050436228
2	14.818641244115504	14.675745845257323
3	5.2365697054049	7.779110597742597
4	2.351986177622095	4.658612190648302
5	1.1880901635385381	2.9415836772932344
6	0.7260536408481247	2.157157028281944
7	0.4870565503535867	1.6882596114089814
8	0.324118613390207	1.2839726167797676
9	0.23232273331203718	1.035371056899426
>10	1.2466343177287376	11.138023374347048
>50	0.09603971321099462	3.264823075555202
>100	0.0779287258122696	7.994006098543721
>500	0.008615130173608977	2.86185805598707
>1k	0.003393839159300506	2.2731462668929727
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2605	0.16796514578149574	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1797	0.11586693549687056	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1764	0.11373916205702819	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1714	0.11051526290575188	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1558	0.1004566975537698	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.2895596605105237E-4
6	0.0	0.0	0.0	0.0	1.9343394907657857E-4
7	0.0	0.0	0.0	0.0	1.9343394907657857E-4
8	0.0	0.0	0.0	0.0	1.9343394907657857E-4
9	0.0	0.0	0.0	0.0	1.9343394907657857E-4
10	0.0	0.0	0.0	0.0	2.5791193210210475E-4
11	0.0	0.0	0.0	6.447798302552619E-5	2.5791193210210475E-4
12	0.0	0.0	0.0	6.447798302552619E-5	3.8686789815315715E-4
13	0.0	0.0	0.0	6.447798302552619E-5	5.158238642042095E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCC	45	3.5426427E-5	16.886793	3
GGTATCA	740	0.0	15.399516	1
TCTTATA	125	1.03682396E-10	12.916728	2
CCGACCA	290	0.0	12.77634	9
CGTTATT	135	2.7284841E-11	12.66346	2
GTATCAA	1900	0.0	12.245317	1
GTCTTAC	70	1.0947962E-4	12.209617	1
TCGCACG	165	0.0	12.091751	13
TCGGAAT	135	3.7107384E-10	11.962252	19
CGACCAT	310	0.0	11.952831	10
CGCCGGT	260	0.0	11.693123	7
CGTCGTA	245	0.0	11.63384	10
CCGTCGT	230	0.0	11.56565	9
ACGGTAT	230	0.0	11.56565	9
ACCGTCG	250	0.0	11.400058	8
ACGCCCC	175	1.8189894E-12	11.399322	17
GCGTTAT	150	1.7826096E-10	11.395642	1
CGTTCGC	60	0.0058725956	11.0841055	15
ACATCGT	95	1.3585372E-5	11.001122	6
GACCATA	340	0.0	10.899226	11
>>END_MODULE