FastQCFastQC Report
Thu 2 Feb 2017
SRR4062044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550917
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17710.11419050793820687No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17050.10993496105852216No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16670.10748479770355215No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16130.10400298662017374No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16080.10368059670504612No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16020.10329372880689294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8700.027.8221071
GTATCAA20000.026.6257531
GGTATCG450.001397419324.44736344
ATCGATT602.87347E-421.9997929
CGGATCG654.9333466E-420.30749926
CTATAAG2150.019.4460641
CGACACG600.007413430618.33315825
ACGGTAT3600.018.3325679
TCAACGC29700.017.8511074
TAGGACG3100.017.7411944
CGACGGT3750.017.5992667
GACGGTA3750.017.5992668
TACGACG3900.017.486455
CAACGCA30350.017.4687945
ATCAACG30300.017.4250143
TATAAGG2400.017.41652
AACGCAG30550.017.2824216
GTCCTAC5350.017.2747611
GTCCTAA2600.016.9267351
CGTCTTA1702.4701876E-916.82282815
Produced by FastQC (version 0.11.5)