##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062044_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1550917
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251201708408637	32.0	32.0	32.0	32.0	32.0
2	31.454110052310988	32.0	32.0	32.0	32.0	32.0
3	31.58005296221526	32.0	32.0	32.0	32.0	32.0
4	31.634100341926743	32.0	32.0	32.0	32.0	32.0
5	31.638225643280716	32.0	32.0	32.0	32.0	32.0
6	35.25861409733725	36.0	36.0	36.0	36.0	36.0
7	35.26679312948404	36.0	36.0	36.0	36.0	36.0
8	35.20814073222487	36.0	36.0	36.0	36.0	36.0
9	35.32820389485704	36.0	36.0	36.0	36.0	36.0
10	35.193608039630746	36.0	36.0	36.0	36.0	36.0
11	35.31744380904974	36.0	36.0	36.0	36.0	36.0
12	35.23767422756988	36.0	36.0	36.0	36.0	36.0
13	35.27339503016602	36.0	36.0	36.0	36.0	36.0
14	35.2208983459463	36.0	36.0	36.0	36.0	36.0
15	35.19221789431672	36.0	36.0	36.0	36.0	36.0
16	35.229675733775565	36.0	36.0	36.0	36.0	36.0
17	35.206367587691666	36.0	36.0	36.0	36.0	36.0
18	35.19419414449645	36.0	36.0	36.0	36.0	36.0
19	35.20119709823285	36.0	36.0	36.0	36.0	36.0
20	35.196585632886865	36.0	36.0	36.0	36.0	36.0
21	35.18750197463823	36.0	36.0	36.0	36.0	36.0
22	35.17698174692779	36.0	36.0	36.0	36.0	36.0
23	35.120615738946704	36.0	36.0	36.0	36.0	36.0
24	35.124243915051544	36.0	36.0	36.0	36.0	36.0
25	35.099056235762454	36.0	36.0	36.0	36.0	36.0
26	35.06641167773646	36.0	36.0	36.0	36.0	36.0
27	35.05748405620675	36.0	36.0	36.0	36.0	36.0
28	35.0289577069566	36.0	36.0	36.0	36.0	36.0
29	34.99534017616674	36.0	36.0	36.0	36.0	36.0
30	34.99319886235047	36.0	36.0	36.0	36.0	36.0
31	34.982574825087354	36.0	36.0	36.0	36.0	36.0
32	34.92908389036938	36.0	36.0	36.0	32.0	36.0
33	34.90058720099141	36.0	36.0	36.0	32.0	36.0
34	34.882230964003874	36.0	36.0	36.0	32.0	36.0
35	34.8587164883743	36.0	36.0	36.0	32.0	36.0
36	34.836478676808625	36.0	36.0	36.0	32.0	36.0
37	34.84091863071976	36.0	36.0	36.0	32.0	36.0
38	34.827408558936426	36.0	36.0	36.0	32.0	36.0
39	34.81717074479163	36.0	36.0	36.0	32.0	36.0
40	34.82441549096438	36.0	36.0	36.0	32.0	36.0
41	34.813977795072205	36.0	36.0	36.0	32.0	36.0
42	34.77914034084351	36.0	36.0	36.0	32.0	36.0
43	34.78713496595885	36.0	36.0	36.0	32.0	36.0
44	34.73801112503119	36.0	36.0	36.0	32.0	36.0
45	34.7222791419528	36.0	36.0	36.0	32.0	36.0
46	34.69992204611852	36.0	36.0	36.0	32.0	36.0
47	34.67769326147047	36.0	36.0	36.0	32.0	36.0
48	34.639147033658155	36.0	36.0	36.0	32.0	36.0
49	34.624454435666124	36.0	36.0	36.0	32.0	36.0
50	34.22417963050247	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	11.0
20	20.0
21	36.0
22	98.0
23	291.0
24	698.0
25	1668.0
26	3649.0
27	6812.0
28	11918.0
29	18974.0
30	28542.0
31	42595.0
32	64651.0
33	110618.0
34	255191.0
35	1005144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.190714405036566	17.748253218637753	12.100633182041289	25.9603991942844
2	16.280416364950597	19.60536505441206	37.306356316628744	26.807862264008598
3	19.004341955775754	23.144481254279732	28.199629379836665	29.65154741010785
4	12.59725697764613	15.754679328423121	35.20543007781848	36.442633616112275
5	14.636695580743522	36.64296670937258	33.00608607681778	15.714251633066114
6	35.40063175690706	34.780094047828605	16.215222904186117	13.604051291078223
7	31.092508496586213	29.902696275816176	19.72497561120292	19.27981961639469
8	28.42292656538035	33.30771408141119	18.566693124132367	19.702666229076087
9	27.126596716652145	14.255372789130561	18.3467587240323	40.271271770184995
10	16.581136401590545	26.661908179246673	30.495649981655966	26.26130543750681
11	37.811759107676295	21.050578464224714	21.604508816397008	19.53315361170198
12	24.788109228282366	23.370883161381297	28.414544427587035	23.426463182749302
13	29.721835533429577	19.249579442355717	25.043635475012525	25.98494954920218
14	23.699785352794507	19.38156587360897	24.65966908609552	32.258979687501004
15	25.31089671465333	27.13697767191926	21.81548077685653	25.736644836570882
16	26.079847109510908	25.672023078004326	23.383759512132844	24.864370300351922
17	24.344952050947924	25.977470103171218	24.681720556290244	24.99585728959061
18	24.992069202974243	25.036752474021807	25.79114536183778	24.180032961166177
19	25.92287396609488	24.80508293163524	24.83874046047626	24.433302641793624
20	26.136170201790947	24.326460817892954	24.510546052903067	25.026822927413033
21	26.998994788244634	24.185046653044616	24.069888975361028	24.746069583349723
22	26.216876854145	24.235210523838475	24.781145606115608	24.766767015900914
23	24.864515638167614	24.2168342986762	25.18464882388935	25.734001239266835
24	25.025823681960564	24.730898535175015	25.11557790240776	25.12769988045666
25	25.23079938668424	24.28322911521851	25.157874646821167	25.32809685127609
26	24.76159158171654	25.22054793637284	25.386321578943978	24.631538902966646
27	25.271630323557805	24.73981454794692	24.8694801062342	25.119075022261082
28	24.929669642367795	24.69167803107238	25.254707765516045	25.123944561043782
29	24.73949148531603	24.76612102966712	25.23952082294964	25.254866662067215
30	24.86646463343865	25.00264362628151	25.261590044490294	24.86930169578954
31	25.429457544280453	24.69131026300688	24.493555396509144	25.385676796203523
32	25.29652041872326	24.72459628410509	24.445920285252804	25.532963011918845
33	24.847186687574798	24.558259914166634	25.053452420253375	25.5411009780052
34	25.491308645445994	24.814286331435305	25.071425854128126	24.62297916899057
35	25.7608260424666	24.468683930046367	25.34158911557257	24.428900911914468
36	24.63860067211893	25.085239948520744	24.840545845165053	25.435613534195276
37	25.649819587695337	24.824552580063603	24.674574373973172	24.851053458267888
38	25.04905162558667	24.5414164652267	24.93086348270088	25.47866842648575
39	25.63295424211201	24.47511190262183	24.55596807535228	25.335965779913884
40	26.021800034173374	24.56543395350487	24.78768984760609	24.62507616471567
41	24.74121577967975	25.06347902451857	25.416949834483844	24.778355361317836
42	25.96106787683939	25.149192089772534	24.73864383020183	24.151096203186246
43	25.393926849571752	24.283381016585	25.101572741300142	25.221119392543102
44	24.819146747929995	24.96306847308206	24.796965263408353	25.42081951557959
45	25.084758002844826	25.014475596529984	24.799308266371607	25.10145813425358
46	24.976384512366778	24.819119872537623	24.71988646511775	25.48460914997785
47	25.347669095363983	24.720484879747243	24.99529305564511	24.936552969243664
48	25.859859961840602	25.14509663329385	24.09916285319851	24.89588055166704
49	25.11885379355259	25.309909694974706	24.368364547298103	25.2028719641746
50	25.247131813735702	25.39749679700663	24.28039845430987	25.0749729349478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	126.0
1	76.0
2	26.0
3	96.0
4	166.0
5	184.0
6	202.0
7	164.5
8	127.0
9	184.5
10	242.0
11	397.0
12	552.0
13	953.0
14	1354.0
15	1866.0
16	2378.0
17	2392.5
18	2407.0
19	2436.0
20	2465.0
21	2925.0
22	3385.0
23	4278.0
24	5171.0
25	6697.5
26	8224.0
27	10940.0
28	13656.0
29	16238.5
30	18821.0
31	21327.0
32	23833.0
33	27324.0
34	30815.0
35	35846.0
36	40877.0
37	48274.5
38	55672.0
39	60847.0
40	66022.0
41	71375.5
42	76729.0
43	79619.5
44	82510.0
45	90196.0
46	97882.0
47	104724.0
48	111566.0
49	116740.0
50	121914.0
51	118985.5
52	116057.0
53	116298.5
54	116540.0
55	117215.5
56	117891.0
57	114348.0
58	110805.0
59	101944.5
60	93084.0
61	81486.0
62	69888.0
63	60505.5
64	51123.0
65	42732.5
66	34342.0
67	29718.0
68	25094.0
69	22291.0
70	19488.0
71	14954.5
72	10421.0
73	9162.5
74	7904.0
75	5797.5
76	3691.0
77	3279.5
78	2868.0
79	2465.5
80	2063.0
81	1582.5
82	1102.0
83	929.0
84	756.0
85	582.5
86	409.0
87	275.0
88	141.0
89	91.5
90	42.0
91	32.5
92	23.0
93	21.5
94	20.0
95	19.0
96	18.0
97	18.0
98	18.0
99	12.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031852123614609935
2	0.0042555468796847285
3	1.9343394907657857E-4
4	0.0
5	0.0
6	2.5791193210210475E-4
7	0.0
8	0.0
9	0.0
10	2.5791193210210475E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.9343394907657857E-4
17	0.0
18	6.447798302552619E-5
19	6.447798302552619E-5
20	3.22389915127631E-4
21	0.0
22	0.0
23	0.0
24	9.671697453828928E-4
25	1.9343394907657857E-4
26	4.513458811786833E-4
27	2.5791193210210475E-4
28	0.0014185156265615762
29	5.158238642042095E-4
30	0.001096125711433945
31	4.513458811786833E-4
32	7.737357963063143E-4
33	3.22389915127631E-4
34	5.158238642042095E-4
35	2.5791193210210475E-4
36	6.447798302552619E-5
37	0.0016119495756381547
38	0.0
39	9.671697453828928E-4
40	1.2895596605105237E-4
41	7.092578132807881E-4
42	0.004126590913633676
43	0.0037397230154805187
44	0.004771370743888938
45	0.001998817473791312
46	0.0018053835247147333
47	0.001096125711433945
48	0.0034818110833784144
49	0.003997634947582624
50	0.0015474715926126286
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1550917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.78536586304373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.4432826196797	36.79058612160311
2	15.4554821803582	15.69824634238488
3	5.3898104305578585	8.211704839449903
4	2.393992859900178	4.8631921305417976
5	1.2136381481671727	3.0817528690008373
6	0.7137713616147938	2.1749483845302118
7	0.4663482942085975	1.6578568118692991
8	0.32504079947065667	1.3205852737226826
9	0.23341717497366993	1.066875896678033
>10	1.1991479303688088	10.721690388794093
>50	0.08889519033530907	3.104478477252037
>100	0.06722359549270383	7.006166482292313
>500	0.007525839454745417	2.6420558264216285
>1k	0.00242357541762988	1.6598601554591488
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1771	0.11419050793820687	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1705	0.10993496105852216	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1667	0.10748479770355215	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1613	0.10400298662017374	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1608	0.10368059670504612	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1602	0.10329372880689294	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.447798302552619E-5
2	0.0	0.0	0.0	0.0	6.447798302552619E-5
3	0.0	0.0	0.0	0.0	6.447798302552619E-5
4	0.0	0.0	0.0	0.0	6.447798302552619E-5
5	0.0	0.0	0.0	0.0	6.447798302552619E-5
6	0.0	0.0	0.0	0.0	2.5791193210210475E-4
7	0.0	0.0	0.0	0.0	2.5791193210210475E-4
8	0.0	0.0	0.0	0.0	2.5791193210210475E-4
9	0.0	0.0	0.0	0.0	3.223899151276309E-4
10	0.0	0.0	0.0	0.0	3.223899151276309E-4
11	0.0	0.0	0.0	0.0	3.8686789815315715E-4
12	0.0	0.0	0.0	0.0	8.382137793318405E-4
13	0.0	0.0	0.0	0.0	9.671697453828928E-4
14	0.0	0.0	0.0	6.447798302552619E-5	9.671697453828928E-4
15	0.0	0.0	0.0	1.9343394907657857E-4	0.0010961257114339453
16	0.0	0.0	0.0	3.223899151276309E-4	0.0011606036944594713
17	0.0	0.0	0.0	3.8686789815315715E-4	0.0012895596605105237
18	0.0	0.0	0.0	3.8686789815315715E-4	0.0015474715926126286
19	0.0	0.0	0.0	4.513458811786833E-4	0.0015474715926126286
20	0.0	0.0	0.0	6.447798302552618E-4	0.0016119495756381547
21	0.0	0.0	0.0	0.001031647728408419	0.0018698615077402596
22	0.0	0.0	0.0	0.0012250816774849976	0.0018698615077402596
23	0.0	0.0	0.0	0.0016119495756381547	0.0019988174737913117
24	0.0	0.0	0.0	0.0018698615077402596	0.0019988174737913117
25	0.0	0.0	0.0	0.0021922514228678905	0.0019988174737913117
26	0.0	0.0	0.0	0.0029015092361486783	0.002063295456816838
27	0.0	0.0	0.0	0.003481811083378414	0.002256729405893417
28	0.0	0.0	0.0	0.004320024862710254	0.002256729405893417
29	0.0	0.0	0.0	0.0054161505741442	0.002256729405893417
30	0.0	0.0	0.0	0.006641232251629197	0.002256729405893417
31	0.0	0.0	0.0	0.009607219470803402	0.0023212073889189427
32	0.0	0.0	0.0	0.012379772740901029	0.0024501633549699952
33	0.0	0.0	0.0	0.01521680399402418	0.0024501633549699952
34	0.0	0.0	0.0	0.019149960958581277	0.0024501633549699952
35	0.0	0.0	0.0	0.022373860109857587	0.0024501633549699952
36	0.0	0.0	0.0	0.028370312531231522	0.0024501633549699952
37	0.0	0.0	0.0	0.03597871452824361	0.0024501633549699952
38	0.0	0.0	0.0	0.04642414777837885	0.0025146413379955215
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	870	0.0	27.822107	1
GTATCAA	2000	0.0	26.625753	1
GGTATCG	45	0.0013974193	24.447363	44
ATCGATT	60	2.87347E-4	21.99979	29
CGGATCG	65	4.9333466E-4	20.307499	26
CTATAAG	215	0.0	19.446064	1
CGACACG	60	0.0074134306	18.333158	25
ACGGTAT	360	0.0	18.332567	9
TCAACGC	2970	0.0	17.851107	4
TAGGACG	310	0.0	17.741194	4
CGACGGT	375	0.0	17.599266	7
GACGGTA	375	0.0	17.599266	8
TACGACG	390	0.0	17.48645	5
CAACGCA	3035	0.0	17.468794	5
ATCAACG	3030	0.0	17.425014	3
TATAAGG	240	0.0	17.4165	2
AACGCAG	3055	0.0	17.282421	6
GTCCTAC	535	0.0	17.274761	1
GTCCTAA	260	0.0	16.926735	1
CGTCTTA	170	2.4701876E-9	16.822828	15
>>END_MODULE