FastQCFastQC Report
Thu 2 Feb 2017
SRR4062043_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062043_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110542
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17221.5577789437498868No Hit
GTACATGGGAAGCAGTGGTATCAAC12041.0891787736787828No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10430.9435327748728991No Hit
TATCAACGCAGAGTACTTTTTTTTT10300.9317725389444735No Hit
GTCCTAAAGTGTGTATTTCTCATTT10020.9064428000217112No Hit
CTGTAGGACGTGGAATATGGCAAGA9390.8494508874454958No Hit
CCCATGTACTCTGCGTTGATACCAC8510.7698431365453855No Hit
GTATCAACGCAGAGTACATGGGAAG8090.7318485281612419No Hit
GTCCTACAGTGGACATTTCTAAATT7810.7065187892384795No Hit
CTTTAGGACGTGAAATATGGCGAGG6960.6296249389372366No Hit
GGTATCAACGCAGAGTACTTTTTTT6610.5979627652837836No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6060.5482079209712146No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5760.5210689149825406No Hit
GTACATGGAAGCAGTGGTATCAACG4720.42698702755513745No Hit
TATCAACGCAGAGTACATGGGAAGC4600.41613142515966783No Hit
CTGAAGGACCTGGAATATGGCGAGA3950.3573302455175409No Hit
GTCCTTCAGTGTGCATTTCTCATTT3860.34918854372093866No Hit
GGTATCAACGCAGAGTACATGGGAA3840.34737927665502705No Hit
TCCATGTACTCTGCGTTGATACCAC3830.34647464312207127No Hit
GCGTTGATACCACTGCTTCCCATGT3820.34557000958911543No Hit
ATTTAGAAATGTCCACTGTAGGACG3800.3437607425232038No Hit
GAGTACATGGGAAGCAGTGGTATCA3780.34195147545729226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3560.32204953773226463No Hit
GTCCTACAGTGTGCATTTCTCATTT3490.3157171030015741No Hit
CATGTACTCTGCGTTGATACCACTG3350.3030522335401929No Hit
TTTCTAAATTTTCCACCTTTTTCAG3280.2967197988095023No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3170.2867688299469885No Hit
ACGCAGAGTACATGGGAAGCAGTGG3030.2741039604856073No Hit
GAATATGGCAAGAAAACTGAAAATC2960.2677715257549167No Hit
AAAAAGTACTCTGCGTTGATACCAC2730.24696495449693331No Hit
CTGTAGGACCTGGAATATGGCGAGA2600.2352047185685079No Hit
GTACATGGGAGTGGTATCAACGCAA2390.2162074143764361No Hit
GCTTCCCATGTACTCTGCGTTGATA2370.2143981473105245No Hit
ACTCTGCGTTGATACCACTGCTTCC2280.2062564455139223No Hit
ATTCCAGGTCCTTCAGTGTGCATTT2260.20444717844801072No Hit
GTATCAACGCAGAGTACATGGGAGT2190.19811474371732013No Hit
GCCATATTCCACGTCCTACAGTGGA2160.1954008431184527No Hit
GAAATATGGCGAGGAAAACTGAAAA2160.1954008431184527No Hit
GGAATATGGCGAGAAAACTGAAAAT2080.18816377485480631No Hit
CCATATTCCAGGTCCTTCAGTGTGC1980.17911743952524833No Hit
CAGTGGTATCAACGCAGAGTACATG1810.16373866946499974No Hit
CCATATTTCACGTCCTAAAGTGTGT1810.16373866946499974No Hit
TTCCAGGTCCTTCAGTGTGCATTTC1760.15921550180022073No Hit
CCACTGTAGGACGTGGAATATGGCA1750.15831086826726493No Hit
ATCAACGCAGAGTACTTTTTTTTTT1640.14835989940475114No Hit
GAGTACATGGAAGCAGTGGTATCAA1620.14655063233883953No Hit
ACAGTGGACATTTCTAAATTTTCCA1600.14474136527292794No Hit
GTCCACTGTAGGACGTGGAATATGG1600.14474136527292794No Hit
CACTTTAGGACGTGAAATATGGCGA1550.14021819760814894No Hit
AAAGTACTCTGCGTTGATACCACTG1550.14021819760814894No Hit
GTGTATTTCTCATTTTCCGTGATTT1540.13931356407519313No Hit
GTGGTATCAACGCAGAGTACATGGG1520.13750429700928155No Hit
CCTAAAGTGTGTATTTCTCATTTTC1500.13569502994336993No Hit
TCCTAAAGTGTGTATTTCTCATTTT1490.13479039641041415No Hit
ATACACACTTTAGGACGTGAAATAT1480.13388576287745835No Hit
GTAGGACGTGGAATATGGCAAGAAA1460.13207649581154673No Hit
GACCTGGAATATGGCGAGAAAACTG1450.13117186227859093No Hit
ATATTTCACGTCCTAAAGTGTGTAT1400.12664869461381195No Hit
GGTATCAACGCAGAGTACATGGGAG1380.12483942754790034No Hit
GGTCCTTCAGTGTGCATTTCTCATT1360.12303016048198874No Hit
ATTCCACGTCCTACAGTGGACATTT1340.12122089341607716No Hit
GTACTCTGCGTTGATACCACTGCTT1300.11760235928425394No Hit
GAAATACACACTTTAGGACGTGAAA1280.11579309221834236No Hit
GTATCAACGCAGAGTACATGGGACG1270.11488845868538657No Hit
ACCTGGAATATGGCGAGAAAACTGA1260.11398382515243075No Hit
CTACAGTGGACATTTCTAAATTTTC1260.11398382515243075No Hit
AGTGTGTATTTCTCATTTTCCGTGA1250.11307919161947495No Hit
CTGTAGGACATGGAATATGGCAAGA1150.10403285628991696No Hit
ATCAACGCAGAGTACATGGGAAGCA1140.10312822275696115No Hit
ACGTGGAATATGGCAAGAAAACTGA1130.10222358922400536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACCT1100.015.54879212
CCACCTT1055.456968E-1215.38425513
CACCTTT1151.8189894E-1214.87275914
AAATGTC902.1937012E-914.7809517
TTCCACC1152.3646862E-1114.046494511
AATGTCC1051.3078534E-913.5743438
TAGGACC707.135057E-613.56819254
TTTCCAC852.6162525E-713.41464410
ATGTCCA1153.583409E-1013.220239
TGTCCAC1102.6211637E-912.95732710
GACGTGG2200.012.9573277
TAGAAAT1102.6375346E-912.9514564
ATTTAGA1259.822543E-1112.9052241
GAAATGT1051.913213E-812.6693876
TTTAGAA1301.891749E-1012.4144892
ACGTGGA2350.012.1302648
ACATTTC2850.012.00257813
CGTGGAA2300.011.9808349
TTAGAAA1353.5470293E-1011.9546933
GACATTT2900.011.79563612
Produced by FastQC (version 0.11.5)