##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062043_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110542
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.941117403339906	32.0	32.0	32.0	32.0	32.0
2	30.878598179877333	32.0	32.0	32.0	32.0	32.0
3	30.935861482513435	32.0	32.0	32.0	32.0	32.0
4	30.832516147708564	32.0	32.0	32.0	32.0	32.0
5	31.02048090318612	32.0	32.0	32.0	32.0	32.0
6	34.55530929420492	36.0	36.0	36.0	32.0	36.0
7	34.414494038465016	36.0	36.0	36.0	32.0	36.0
8	34.34869099527781	36.0	36.0	36.0	32.0	36.0
9	34.201479980459915	36.0	36.0	36.0	32.0	36.0
10	34.25807385428163	36.0	36.0	36.0	32.0	36.0
11	34.37679795914675	36.0	36.0	36.0	32.0	36.0
12	34.30912232454633	36.0	36.0	36.0	32.0	36.0
13	34.26217184418592	36.0	36.0	36.0	32.0	36.0
14	34.23718586600568	36.0	36.0	36.0	32.0	36.0
15	34.261221978976316	36.0	36.0	36.0	32.0	36.0
16	34.23774673879611	36.0	36.0	36.0	32.0	36.0
17	34.13372292884153	36.0	36.0	36.0	32.0	36.0
18	34.14101427511715	36.0	36.0	36.0	32.0	36.0
19	34.05127462864793	36.0	36.0	36.0	32.0	36.0
20	33.94658138987896	36.0	36.0	36.0	32.0	36.0
21	33.86967849324239	36.0	36.0	36.0	32.0	36.0
22	33.76354688715601	36.0	36.0	36.0	27.0	36.0
23	33.81654031951656	36.0	36.0	36.0	32.0	36.0
24	33.76071538419786	36.0	36.0	36.0	27.0	36.0
25	33.30895044417507	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3.0
5	4.0
6	13.0
7	6.0
8	22.0
9	10.0
10	0.0
11	2.0
12	3.0
13	8.0
14	109.0
15	133.0
16	157.0
17	182.0
18	217.0
19	293.0
20	330.0
21	400.0
22	586.0
23	656.0
24	823.0
25	953.0
26	1251.0
27	1465.0
28	1818.0
29	2321.0
30	3116.0
31	4288.0
32	6048.0
33	8661.0
34	20378.0
35	56286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24150315267638	20.137324612587186	13.11278168281452	22.508390551921913
2	10.909074461059697	22.151057073845905	46.52120027862965	20.41866818646475
3	18.39515107653338	31.52071648272119	28.937036366926	21.147096073819434
4	10.431179546564858	18.82769102719525	38.612553603416146	32.12857582282375
5	9.091073609917204	41.92372076188753	35.393385513278744	13.591820114916528
6	26.930596285434994	37.928568842547335	21.289055428840374	13.851779443177293
7	24.15900756100874	34.02182279168742	23.88735455245167	17.931815094852173
8	24.23400094165369	39.301365397848684	20.968997863170475	15.495635797327154
9	28.319689944943494	15.073529411764705	21.87318893074471	34.73359171254709
10	16.358877285777684	29.730733350843664	34.06182355018974	19.848565813188905
11	30.079069641061867	22.885815467941924	27.632711101450063	19.402403789546142
12	27.38568543457195	25.696664523315732	32.59583948414675	14.321810557965566
13	30.732798435151143	24.461630413127345	26.181334106098202	18.624237045623314
14	20.267651799134388	26.408431574944313	29.255174661813438	24.068741964107858
15	21.225376986822443	36.30213286238283	26.021826744554634	16.450663406240093
16	15.805248193556565	29.132182763179344	36.625980187978776	18.43658885528532
17	17.683390690092374	31.6002535772505	32.669806194529976	18.046549538127152
18	18.426199228582295	25.04300744255111	40.62166126432827	15.909132064538326
19	22.178865027342194	26.43953934740883	33.02520551913953	18.35639010610944
20	23.562375520550425	26.928299837045085	34.205142132898786	15.304182509505704
21	19.844826493567634	26.56870999574495	31.193133979738725	22.393329530948694
22	22.005631558456844	32.50278409039466	29.285009370840843	16.206574980307654
23	15.310389774969677	33.208718793562284	34.06502887557254	17.415862555895504
24	20.918801484565947	29.15180592015932	34.71530732325518	15.214085272019554
25	18.94903593735856	28.957182945596088	34.13053317642799	17.963247940617364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	441.0
1	441.0
2	397.0
3	353.0
4	353.0
5	353.0
6	827.5
7	1302.0
8	1302.0
9	1302.0
10	1136.0
11	970.0
12	970.0
13	970.0
14	782.5
15	595.0
16	595.0
17	595.0
18	1094.5
19	1594.0
20	1594.0
21	1594.0
22	2657.0
23	3720.0
24	3720.0
25	3720.0
26	5985.0
27	8250.0
28	8250.0
29	8250.0
30	10758.0
31	13266.0
32	13266.0
33	13266.0
34	13286.0
35	13306.0
36	13306.0
37	13306.0
38	13007.5
39	12709.0
40	12709.0
41	12709.0
42	12973.0
43	13237.0
44	13237.0
45	13237.0
46	16339.5
47	19442.0
48	19442.0
49	19442.0
50	15790.5
51	12139.0
52	12139.0
53	12139.0
54	8446.5
55	4754.0
56	4754.0
57	4754.0
58	3522.5
59	2291.0
60	2291.0
61	2291.0
62	1744.5
63	1198.0
64	1198.0
65	1198.0
66	875.5
67	553.0
68	553.0
69	553.0
70	390.0
71	227.0
72	227.0
73	227.0
74	155.5
75	84.0
76	84.0
77	84.0
78	61.5
79	39.0
80	39.0
81	39.0
82	28.5
83	18.0
84	18.0
85	18.0
86	13.5
87	9.0
88	9.0
89	9.0
90	6.0
91	3.0
92	3.0
93	3.0
94	3.0
95	3.0
96	3.0
97	3.0
98	21.0
99	39.0
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.046335329557997E-4
2	9.046335329557997E-4
3	0.0018092670659115993
4	0.007237068263646397
5	0.02442510538980659
6	0.052468744911436375
7	0.09679578802627056
8	0.08865408622966836
9	0.09950968862513795
10	0.11850699281720975
11	0.12031625988312133
12	0.11126992455356335
13	0.10493748982287275
14	0.09046335329557996
15	0.11488845868538657
16	0.09408188742740316
17	0.11036529102060755
18	0.08684481916375676
19	0.08141701796602197
20	0.07417994970237557
21	0.07689385030124297
22	0.08413091856488937
23	0.06151508024099437
24	0.06513361437281757
25	0.06513361437281757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	110542.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.47259865028677
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.99605032182563	40.56557688480397
2	11.156012287887654	11.038338369126667
3	2.9165447630193095	4.328671455193501
4	1.1190754827384435	2.2145428886757976
5	0.5686805149210064	1.4067051437462685
6	0.43336746635459333	1.286388883863147
7	0.26148332358104154	0.9055381664887554
8	0.1901696898771211	0.7526550994192253
9	0.1298273844353423	0.578060827558756
>10	0.9673054417788179	9.641584194242911
>50	0.12068461088355763	4.254491505491126
>100	0.11702750146284377	12.244214868556748
>500	0.014628437682855471	5.354525881565378
>1k	0.00914277355178467	5.428705831267753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1722	1.5577789437498868	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1204	1.0891787736787828	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1043	0.9435327748728991	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1030	0.9317725389444735	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1002	0.9064428000217112	No Hit
CTGTAGGACGTGGAATATGGCAAGA	939	0.8494508874454958	No Hit
CCCATGTACTCTGCGTTGATACCAC	851	0.7698431365453855	No Hit
GTATCAACGCAGAGTACATGGGAAG	809	0.7318485281612419	No Hit
GTCCTACAGTGGACATTTCTAAATT	781	0.7065187892384795	No Hit
CTTTAGGACGTGAAATATGGCGAGG	696	0.6296249389372366	No Hit
GGTATCAACGCAGAGTACTTTTTTT	661	0.5979627652837836	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	606	0.5482079209712146	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	576	0.5210689149825406	No Hit
GTACATGGAAGCAGTGGTATCAACG	472	0.42698702755513745	No Hit
TATCAACGCAGAGTACATGGGAAGC	460	0.41613142515966783	No Hit
CTGAAGGACCTGGAATATGGCGAGA	395	0.3573302455175409	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	386	0.34918854372093866	No Hit
GGTATCAACGCAGAGTACATGGGAA	384	0.34737927665502705	No Hit
TCCATGTACTCTGCGTTGATACCAC	383	0.34647464312207127	No Hit
GCGTTGATACCACTGCTTCCCATGT	382	0.34557000958911543	No Hit
ATTTAGAAATGTCCACTGTAGGACG	380	0.3437607425232038	No Hit
GAGTACATGGGAAGCAGTGGTATCA	378	0.34195147545729226	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	356	0.32204953773226463	No Hit
GTCCTACAGTGTGCATTTCTCATTT	349	0.3157171030015741	No Hit
CATGTACTCTGCGTTGATACCACTG	335	0.3030522335401929	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	328	0.2967197988095023	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	317	0.2867688299469885	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	303	0.2741039604856073	No Hit
GAATATGGCAAGAAAACTGAAAATC	296	0.2677715257549167	No Hit
AAAAAGTACTCTGCGTTGATACCAC	273	0.24696495449693331	No Hit
CTGTAGGACCTGGAATATGGCGAGA	260	0.2352047185685079	No Hit
GTACATGGGAGTGGTATCAACGCAA	239	0.2162074143764361	No Hit
GCTTCCCATGTACTCTGCGTTGATA	237	0.2143981473105245	No Hit
ACTCTGCGTTGATACCACTGCTTCC	228	0.2062564455139223	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	226	0.20444717844801072	No Hit
GTATCAACGCAGAGTACATGGGAGT	219	0.19811474371732013	No Hit
GCCATATTCCACGTCCTACAGTGGA	216	0.1954008431184527	No Hit
GAAATATGGCGAGGAAAACTGAAAA	216	0.1954008431184527	No Hit
GGAATATGGCGAGAAAACTGAAAAT	208	0.18816377485480631	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	198	0.17911743952524833	No Hit
CAGTGGTATCAACGCAGAGTACATG	181	0.16373866946499974	No Hit
CCATATTTCACGTCCTAAAGTGTGT	181	0.16373866946499974	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	176	0.15921550180022073	No Hit
CCACTGTAGGACGTGGAATATGGCA	175	0.15831086826726493	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	164	0.14835989940475114	No Hit
GAGTACATGGAAGCAGTGGTATCAA	162	0.14655063233883953	No Hit
ACAGTGGACATTTCTAAATTTTCCA	160	0.14474136527292794	No Hit
GTCCACTGTAGGACGTGGAATATGG	160	0.14474136527292794	No Hit
CACTTTAGGACGTGAAATATGGCGA	155	0.14021819760814894	No Hit
AAAGTACTCTGCGTTGATACCACTG	155	0.14021819760814894	No Hit
GTGTATTTCTCATTTTCCGTGATTT	154	0.13931356407519313	No Hit
GTGGTATCAACGCAGAGTACATGGG	152	0.13750429700928155	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	150	0.13569502994336993	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	149	0.13479039641041415	No Hit
ATACACACTTTAGGACGTGAAATAT	148	0.13388576287745835	No Hit
GTAGGACGTGGAATATGGCAAGAAA	146	0.13207649581154673	No Hit
GACCTGGAATATGGCGAGAAAACTG	145	0.13117186227859093	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	140	0.12664869461381195	No Hit
GGTATCAACGCAGAGTACATGGGAG	138	0.12483942754790034	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	136	0.12303016048198874	No Hit
ATTCCACGTCCTACAGTGGACATTT	134	0.12122089341607716	No Hit
GTACTCTGCGTTGATACCACTGCTT	130	0.11760235928425394	No Hit
GAAATACACACTTTAGGACGTGAAA	128	0.11579309221834236	No Hit
GTATCAACGCAGAGTACATGGGACG	127	0.11488845868538657	No Hit
ACCTGGAATATGGCGAGAAAACTGA	126	0.11398382515243075	No Hit
CTACAGTGGACATTTCTAAATTTTC	126	0.11398382515243075	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	125	0.11307919161947495	No Hit
CTGTAGGACATGGAATATGGCAAGA	115	0.10403285628991696	No Hit
ATCAACGCAGAGTACATGGGAAGCA	114	0.10312822275696115	No Hit
ACGTGGAATATGGCAAGAAAACTGA	113	0.10222358922400536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCACCT	110	0.0	15.548792	12
CCACCTT	105	5.456968E-12	15.384255	13
CACCTTT	115	1.8189894E-12	14.872759	14
AAATGTC	90	2.1937012E-9	14.780951	7
TTCCACC	115	2.3646862E-11	14.0464945	11
AATGTCC	105	1.3078534E-9	13.574343	8
TAGGACC	70	7.135057E-6	13.5681925	4
TTTCCAC	85	2.6162525E-7	13.414644	10
ATGTCCA	115	3.583409E-10	13.22023	9
TGTCCAC	110	2.6211637E-9	12.957327	10
GACGTGG	220	0.0	12.957327	7
TAGAAAT	110	2.6375346E-9	12.951456	4
ATTTAGA	125	9.822543E-11	12.905224	1
GAAATGT	105	1.913213E-8	12.669387	6
TTTAGAA	130	1.891749E-10	12.414489	2
ACGTGGA	235	0.0	12.130264	8
ACATTTC	285	0.0	12.002578	13
CGTGGAA	230	0.0	11.980834	9
TTAGAAA	135	3.5470293E-10	11.954693	3
GACATTT	290	0.0	11.795636	12
>>END_MODULE