##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110542
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.73022923413725	32.0	32.0	32.0	32.0	32.0
2	31.394881583470536	32.0	32.0	32.0	32.0	32.0
3	31.594479926181904	32.0	32.0	32.0	32.0	32.0
4	31.718288071502233	32.0	32.0	32.0	32.0	32.0
5	31.73450815074813	32.0	32.0	32.0	32.0	32.0
6	35.38318467188942	36.0	36.0	36.0	36.0	36.0
7	35.365390530296175	36.0	36.0	36.0	36.0	36.0
8	35.36249570299072	36.0	36.0	36.0	36.0	36.0
9	35.31568996399559	36.0	36.0	36.0	36.0	36.0
10	35.29444916864178	36.0	36.0	36.0	36.0	36.0
11	35.35778256228402	36.0	36.0	36.0	36.0	36.0
12	35.30942989994753	36.0	36.0	36.0	36.0	36.0
13	35.27510810370719	36.0	36.0	36.0	36.0	36.0
14	35.25668976497621	36.0	36.0	36.0	36.0	36.0
15	35.228012881981506	36.0	36.0	36.0	36.0	36.0
16	35.22771435291563	36.0	36.0	36.0	36.0	36.0
17	35.209884025981076	36.0	36.0	36.0	36.0	36.0
18	35.18031155578875	36.0	36.0	36.0	36.0	36.0
19	35.114463280924895	36.0	36.0	36.0	36.0	36.0
20	35.035959182934995	36.0	36.0	36.0	36.0	36.0
21	34.989623853377	36.0	36.0	36.0	36.0	36.0
22	34.87940330372166	36.0	36.0	36.0	36.0	36.0
23	34.87619185467967	36.0	36.0	36.0	36.0	36.0
24	34.857891118307975	36.0	36.0	36.0	36.0	36.0
25	34.72230464438856	36.0	36.0	36.0	32.0	36.0
26	34.67527274701018	36.0	36.0	36.0	32.0	36.0
27	34.54111559407284	36.0	36.0	36.0	32.0	36.0
28	34.445613431998694	36.0	36.0	36.0	32.0	36.0
29	34.30863382243853	36.0	36.0	36.0	32.0	36.0
30	34.26083298655715	36.0	36.0	36.0	32.0	36.0
31	34.15462900978814	36.0	36.0	36.0	32.0	36.0
32	34.0976009118706	36.0	36.0	36.0	32.0	36.0
33	34.11848890014655	36.0	36.0	36.0	32.0	36.0
34	33.98644858967632	36.0	36.0	36.0	32.0	36.0
35	33.869470427529805	36.0	36.0	36.0	32.0	36.0
36	33.66941976805196	36.0	36.0	36.0	27.0	36.0
37	33.52302292341373	36.0	36.0	36.0	21.0	36.0
38	33.39909717573411	36.0	36.0	36.0	21.0	36.0
39	32.9826762678439	36.0	36.0	36.0	14.0	36.0
40	32.96321760055002	36.0	36.0	36.0	14.0	36.0
41	32.89860867362631	36.0	36.0	36.0	14.0	36.0
42	32.80404733042644	36.0	36.0	36.0	14.0	36.0
43	32.74026162001773	36.0	36.0	36.0	14.0	36.0
44	32.62737240144018	36.0	36.0	36.0	14.0	36.0
45	32.36034267518228	36.0	36.0	36.0	14.0	36.0
46	32.148721752817934	36.0	36.0	36.0	14.0	36.0
47	32.07990627996599	36.0	36.0	36.0	14.0	36.0
48	31.96972191565197	36.0	36.0	36.0	14.0	36.0
49	31.87333321271553	36.0	36.0	36.0	14.0	36.0
50	31.56368620072009	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	3.0
20	15.0
21	27.0
22	47.0
23	106.0
24	253.0
25	567.0
26	1156.0
27	1948.0
28	2958.0
29	3617.0
30	4528.0
31	5587.0
32	7244.0
33	9307.0
34	16316.0
35	56861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10250052037612	21.39515099957465	13.517109061784483	22.98523941826475
2	10.613544663373682	22.366968825200384	46.57583817329787	20.443648338128064
3	18.222937887861626	31.35550288578097	29.63036673843426	20.791192487923144
4	10.315536176294984	19.04705903638436	38.58352481409781	32.05387997322285
5	8.890738361889598	41.711747571058964	35.40916574695591	13.988348320095529
6	27.48005283059832	38.10859220929601	20.68173183043549	13.729623129670172
7	24.215230410160842	33.52752799840785	24.39525248321905	17.861989108212263
8	24.576179189810208	39.08469179135532	21.003781368167758	15.335347650666714
9	28.252609867742578	14.627924227895281	21.950932677172478	35.16853322718966
10	16.54484268422862	29.232327983933708	34.15624830381213	20.066581028025546
11	30.224710969586223	22.317309258019577	28.164860415045865	19.29311935734834
12	27.385970943170925	25.525139765880844	32.88885672414105	14.200032566807186
13	30.873333212715533	23.996309095185538	26.570896129977744	18.559461562121186
14	20.192325089106404	25.861663440140397	29.446726131244233	24.499285339508965
15	21.366539414883032	36.15277451104558	26.090535723978213	16.390150350093176
16	15.98668379439489	28.418157804273488	36.972372491903535	18.622785909428092
17	17.74167284832914	31.14834180673409	33.1928135912142	17.917171753722567
18	18.907745472309166	24.341879104774655	41.003419514754576	15.746955908161603
19	22.434006983770875	25.5893687467207	33.671364730147815	18.305259539360605
20	23.88163667779376	26.40106385865878	34.57450176857456	15.142797694972906
21	20.19594362323823	25.554992672468384	31.5943261384813	22.654737565812088
22	22.433102350237917	31.42877820195039	29.759729333646938	16.378390114164752
23	15.434857339291852	32.194098170831	34.69179135532196	17.679253134555193
24	21.421722060393332	28.50862115756907	34.84376978885853	15.225886993179063
25	19.34468346872682	27.955890068933076	34.11011199363138	18.589314468708725
26	15.783141249479835	29.999457219880227	36.09940113260118	18.118000398038756
27	17.895460548931627	30.36311990012846	33.27151670858136	18.46990284235856
28	16.148613196793978	30.56756707857647	33.1620560510937	20.12176367353585
29	19.502994336994085	27.57413471802573	33.0435490582765	19.879321886703696
30	16.745974307942827	28.46842771847295	35.90103130088656	18.884566672697666
31	22.034158962204412	25.754916683251615	33.39002370139856	18.82090065314541
32	16.24374666413367	30.51808831112438	34.83503858296921	18.403126441772734
33	15.007870311736715	32.03578730256373	37.07459608112754	15.881746304572017
34	16.342204772846518	30.4011145085126	36.48567965117331	16.77100106746757
35	15.643827685404643	32.282752257060665	35.863291780499715	16.210128277034975
36	16.503229541712653	30.449964719292215	36.46577771344828	16.58102802554685
37	16.422717157279585	31.443252338477684	32.06473557561832	20.069294928624416
38	18.583886667510992	33.509435327748726	31.49481644985616	16.411861554884116
39	20.95873929130368	28.99648094372224	31.983607892094334	18.061171872879747
40	20.86356317055961	29.347216442619096	33.94637332416638	15.842847062654918
41	20.200649532752553	28.669000642295618	34.182791905265915	16.947557919685906
42	18.639912427512957	32.8514434080896	34.11527361878828	14.393370545609162
43	16.38094031862634	33.05770918335037	33.686457928114564	16.874892569908717
44	15.514121840453058	33.01579547304999	33.21482205214497	18.255260634351988
45	17.617897431722167	32.63766385323093	31.84881627631377	17.895622438733138
46	16.60922191765951	30.512660460824492	33.68342967767616	19.194687943839842
47	20.88727259568848	30.34530174324459	31.535810242353516	17.23161541871342
48	16.361646115850515	32.68258261789956	31.140140583866327	19.815630682383592
49	19.043224954314354	31.76011869221444	30.912446398523585	18.28420995494762
50	16.360741457766036	36.60337075602276	29.24126326454916	17.79462452166204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	229.0
1	161.5
2	94.0
3	266.0
4	438.0
5	403.0
6	368.0
7	310.5
8	253.0
9	313.0
10	373.0
11	539.0
12	705.0
13	1055.5
14	1406.0
15	1729.0
16	2052.0
17	1896.0
18	1740.0
19	1576.5
20	1413.0
21	1387.5
22	1362.0
23	1349.5
24	1337.0
25	1359.5
26	1382.0
27	1780.5
28	2179.0
29	3033.5
30	3888.0
31	5196.5
32	6505.0
33	8431.5
34	10358.0
35	11771.0
36	13184.0
37	12429.0
38	11674.0
39	10121.5
40	8569.0
41	7705.0
42	6841.0
43	6373.5
44	5906.0
45	5855.0
46	5804.0
47	5947.5
48	6091.0
49	5787.0
50	5483.0
51	4645.5
52	3808.0
53	2938.5
54	2069.0
55	1784.0
56	1499.0
57	1355.5
58	1212.0
59	1034.0
60	856.0
61	710.5
62	565.0
63	466.5
64	368.0
65	289.5
66	211.0
67	172.5
68	134.0
69	104.5
70	75.0
71	61.5
72	48.0
73	42.5
74	37.0
75	23.0
76	9.0
77	5.5
78	2.0
79	4.0
80	6.0
81	5.0
82	4.0
83	3.5
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04070850898301098
2	0.0036185341318231987
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	9.046335329557997E-4
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0018092670659115993
31	0.0
32	9.046335329557997E-4
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	9.046335329557997E-4
40	0.0
41	9.046335329557997E-4
42	0.004523167664778998
43	0.004523167664778998
44	0.0036185341318231987
45	9.046335329557997E-4
46	9.046335329557997E-4
47	9.046335329557997E-4
48	0.002713900598867399
49	0.0036185341318231987
50	0.002713900598867399
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	110542.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.46228582801108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.2887933841245	59.24354543974236
2	9.974604414924789	13.857176457816939
3	2.2960213583382174	4.784606755803225
4	0.8217750862798724	2.283295037180438
5	0.41153871198801845	1.4293209820701636
6	0.21228104447483234	0.884731595230772
7	0.15497818584358924	0.753559732952181
8	0.10679169108549846	0.5934395976190046
9	0.09507065182001694	0.5943442311519603
>10	0.5469818323891384	7.190027319932696
>50	0.057302858631243084	2.9635794539631997
>100	0.03255844240411539	4.855168171373776
>500	0.0013023376961646155	0.5672052251632863
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.5672052251632863	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	439	0.39713412096759604	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	363	0.32838197246295525	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	349	0.3157171030015741	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	336	0.3039568670731487	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	336	0.3039568670731487	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.29762443234245806	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	314	0.28405492934812104	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	291	0.2632483580901377	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	258	0.2333954515025963	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	217	0.1963054766514085	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	199	0.1800220730582041	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	187	0.16916647066273452	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	186	0.16826183712977874	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	184	0.16645257006386713	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	179	0.16192940239908815	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	155	0.14021819760814894	No Hit
ATACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.11760235928425394	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	127	0.11488845868538657	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	123	0.11126992455356335	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT	120	0.10855602395469595	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	118	0.10674675688878436	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	114	0.10312822275696115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	9.046335329557996E-4	0.0
15	0.0	0.0	0.0	9.046335329557996E-4	0.0
16	0.0	0.0	0.0	9.046335329557996E-4	0.0
17	0.0	0.0	0.0	9.046335329557996E-4	0.0
18	0.0	0.0	0.0	9.046335329557996E-4	0.0
19	0.0	0.0	0.0	0.0018092670659115991	0.0
20	0.0	0.0	0.0	0.0036185341318231983	0.0
21	0.0	0.0	0.0	0.006332434730690597	0.0
22	0.0	0.0	0.0	0.0072370682636463965	0.0
23	0.0	0.0	0.0	0.009950968862513796	0.0
24	0.0	0.0	0.0	0.017188037126160193	0.0
25	0.0	0.0	0.0	0.02080657125798339	0.0
26	0.0	0.0	0.0	0.028948273054585586	0.0
27	0.0	0.0	0.0	0.036185341318231985	0.0
28	0.0	0.0	0.0	0.055182645510303774	0.0
29	0.0	0.0	0.0	0.07237068263646397	0.0
30	0.0	0.0	0.0	0.09770042155922636	0.0
31	0.0	0.0	0.0	0.12664869461381195	0.0
32	0.0	0.0	0.0	0.1682618371297787	0.0
33	0.0	0.0	0.0	0.2089703461127897	0.0
34	0.0	0.0	0.0	0.2551066562935355	0.0
35	0.0	0.0	0.0	0.31843100360044146	0.0
36	0.0	0.0	0.0	0.3962294874346402	0.0
37	0.0	0.0	0.0	0.4812650395324854	0.0
38	0.0	0.0	0.0	0.6422898083986177	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	30	2.499295E-6	44.019466	1
CCTACAC	40	1.8053515E-5	32.99966	3
GGATAAT	35	3.1993145E-4	31.428247	41
TATATAC	30	0.0057230284	29.333035	3
CTGTTCT	30	0.0057230284	29.333035	9
ACTGTTC	30	0.0057230284	29.333035	8
TAGGACC	125	0.0	28.15971	4
TGGATAA	40	6.991923E-4	27.499718	40
ATGGGCA	45	0.0013902831	24.444193	5
GATAATT	45	0.0013902831	24.444193	42
ATATACA	55	1.5809902E-4	23.999754	2
TCACAGA	60	2.8501687E-4	21.999775	36
CACAGAA	60	2.8501687E-4	21.999775	37
GACATGA	50	0.00256592	21.999775	7
ATACACT	50	0.00256592	21.999775	4
TGTAGGA	490	0.0	21.999773	2
CTGTAGG	480	0.0	20.634125	1
AATCACA	75	5.4156204E-5	20.533123	34
GTAGGAC	550	0.0	20.39979	3
AGGACCT	305	0.0	20.196512	5
>>END_MODULE