FastQCFastQC Report
Thu 2 Feb 2017
SRR4062042_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062042_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435742
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24930.17363843921818822No Hit
TATCAACGCAGAGTACTTTTTTTTT17060.11882357693791781No Hit
GCGCAAGACGGACCAGAGCGAAAGC16770.1168037154307668No Hit
GGGTAGGCACACGCTGAGCCAGTCA14560.10141097773834018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCG456.7570823E-414.7791619
GGTATCA6900.014.4503151
CGCGTAA1350.014.07539110
GCGTAAC1400.013.57222611
ACAATAC1051.9950676E-812.6656443
TCGCGTA1600.012.4699179
CGTCGTA2750.012.43752810
CCGGTCC2600.012.424249
ATAATAC701.0916097E-412.21333
AACCGCT550.003066096512.0920417
ACTGCGT550.003066096512.0920418
CTATTAG550.003078129112.0857191
CGAACGA658.0188044E-411.69299516
CGGTCCA2450.011.63374110
TGTAGGA2700.011.6089592
GTATCAA17350.011.5483551
TACCCGG752.0724715E-411.4006695
GCTATAC752.0751581E-411.3990793
ACCATAC2750.011.3951061
CCGTCGT2950.011.2722429
Produced by FastQC (version 0.11.5)