FastQCFastQC Report
Thu 2 Feb 2017
SRR4062042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435742
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16870.11750021939874991No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15130.10538105035584387No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14420.10043587218316383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18250.025.562031
GGTATCA8400.024.3627341
ATACCGT4850.019.9577836
TATACAA1900.019.6832455
GTATAGG1851.8189894E-1219.0313951
ACCGTCG4900.018.8562188
TACCGTC5150.018.368037
TCTATAC1203.1646232E-718.3324343
TAGCCCG750.001291455417.5991365
GGCGTTA4150.017.49616242
GTAGGAC3900.017.486323
CGTCGTA5300.017.43310510
ACGACGG4700.017.31836
CGTATAT3050.017.31062510
CGACGGT4450.017.3025237
GTAATAC1408.379902E-817.2848663
TAGCGTA2850.016.9816237
TACGACG4800.016.95755
TCAACGC27350.016.730444
GACGGTA4650.016.5583298
Produced by FastQC (version 0.11.5)