##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062042_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1435742
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295831702353208	32.0	32.0	32.0	32.0	32.0
2	31.475340973517525	32.0	32.0	32.0	32.0	32.0
3	31.59218787219431	32.0	32.0	32.0	32.0	32.0
4	31.66064585419943	32.0	32.0	32.0	32.0	32.0
5	31.63816200960897	32.0	32.0	32.0	32.0	32.0
6	35.28974774019287	36.0	36.0	36.0	36.0	36.0
7	35.2961472186507	36.0	36.0	36.0	36.0	36.0
8	35.24574679851951	36.0	36.0	36.0	36.0	36.0
9	35.32050814143488	36.0	36.0	36.0	36.0	36.0
10	35.21108318904093	36.0	36.0	36.0	36.0	36.0
11	35.32478746181417	36.0	36.0	36.0	36.0	36.0
12	35.249180563081666	36.0	36.0	36.0	36.0	36.0
13	35.28527757772636	36.0	36.0	36.0	36.0	36.0
14	35.253257200806274	36.0	36.0	36.0	36.0	36.0
15	35.22792953051454	36.0	36.0	36.0	36.0	36.0
16	35.23175403380273	36.0	36.0	36.0	36.0	36.0
17	35.2188109005657	36.0	36.0	36.0	36.0	36.0
18	35.212226848556355	36.0	36.0	36.0	36.0	36.0
19	35.20375805680965	36.0	36.0	36.0	36.0	36.0
20	35.20717371226864	36.0	36.0	36.0	36.0	36.0
21	35.19539652667401	36.0	36.0	36.0	36.0	36.0
22	35.17782024904196	36.0	36.0	36.0	36.0	36.0
23	35.14656672299062	36.0	36.0	36.0	36.0	36.0
24	35.133991343848685	36.0	36.0	36.0	36.0	36.0
25	35.11801075680728	36.0	36.0	36.0	36.0	36.0
26	35.08055973844883	36.0	36.0	36.0	36.0	36.0
27	35.070249390210776	36.0	36.0	36.0	36.0	36.0
28	35.045357731402994	36.0	36.0	36.0	36.0	36.0
29	35.02404192396684	36.0	36.0	36.0	36.0	36.0
30	35.005980183069106	36.0	36.0	36.0	36.0	36.0
31	35.01168037154308	36.0	36.0	36.0	36.0	36.0
32	34.99493154062499	36.0	36.0	36.0	36.0	36.0
33	34.97412418108546	36.0	36.0	36.0	36.0	36.0
34	34.95563269723947	36.0	36.0	36.0	36.0	36.0
35	34.94790219969883	36.0	36.0	36.0	36.0	36.0
36	34.921923994701	36.0	36.0	36.0	36.0	36.0
37	34.91883848212283	36.0	36.0	36.0	36.0	36.0
38	34.89559126918346	36.0	36.0	36.0	32.0	36.0
39	34.88262445481152	36.0	36.0	36.0	32.0	36.0
40	34.868259060471864	36.0	36.0	36.0	32.0	36.0
41	34.84352132904101	36.0	36.0	36.0	32.0	36.0
42	34.820614010038014	36.0	36.0	36.0	32.0	36.0
43	34.81261257245382	36.0	36.0	36.0	32.0	36.0
44	34.76559437559116	36.0	36.0	36.0	32.0	36.0
45	34.75440364633757	36.0	36.0	36.0	32.0	36.0
46	34.738369428490635	36.0	36.0	36.0	32.0	36.0
47	34.707990711423086	36.0	36.0	36.0	32.0	36.0
48	34.683363027619166	36.0	36.0	36.0	32.0	36.0
49	34.67533024735642	36.0	36.0	36.0	32.0	36.0
50	34.26565148891653	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	13.0
21	31.0
22	112.0
23	302.0
24	696.0
25	1630.0
26	3417.0
27	6254.0
28	10703.0
29	16958.0
30	25394.0
31	37737.0
32	57424.0
33	97885.0
34	227457.0
35	949726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86162316118325	17.897049950602593	12.253204561004056	25.9881223272101
2	16.340686008185738	19.51837054249311	37.04735356642344	27.093589882897717
3	18.791855622783807	22.74104137311865	28.2431556153312	30.22394738876634
4	12.900098276778332	15.6646637520277	34.62316671321638	36.812071257977586
5	15.058008373376536	36.135138580008515	32.814135697176575	15.992717349438374
6	35.71937517891483	34.28944954479888	16.07903664941887	13.912138626867417
7	31.449452617531566	29.72706795510614	19.618079014196145	19.205400413166153
8	28.64881016227149	32.98392050939514	18.483822302335657	19.883447025997707
9	27.02212514504695	14.550455443944665	18.47992188011495	39.947497530893436
10	17.07333211677307	26.216130753296902	30.233008437449065	26.477528692480963
11	37.47811236280613	21.129005071941894	21.582568455892492	19.81031410935948
12	24.844853741131764	23.278416317137758	27.976474881977403	23.900255059753075
13	29.579478764290517	19.145918974300397	24.970920959336706	26.30368130207238
14	23.82670424073406	19.226713434586436	24.56792376346168	32.37865856121782
15	25.37370920402133	26.988692954583758	21.653890462213962	25.983707379180938
16	26.213633482618597	25.658515180430864	23.15644306380726	24.97140827314328
17	24.50983533253189	25.994085288303886	24.500432528964115	24.995646850200107
18	25.253057832934818	24.761255353514812	25.62276291164341	24.362923901906964
19	25.894694172072697	24.85850521890423	24.87953963873732	24.36726097028575
20	26.128150389869997	24.11015960466242	24.56379485072106	25.197895154746526
21	26.904833876838595	23.96433342480752	24.316276879829385	24.814555818524497
22	26.435808104798774	23.871419795478573	24.712169735230983	24.98060236449167
23	24.796812238419047	24.048348553116476	25.3221855473933	25.83265366107118
24	25.086141148200387	24.535757195841825	25.081753121245363	25.296348534712426
25	25.290738776337484	24.13725614474497	25.07816532113427	25.493839757783277
26	24.990597157137522	25.07062579750038	25.267040737294323	24.67173630806778
27	25.598333400662238	24.48831193435014	24.730765641084933	25.18258902390269
28	25.11283474307111	24.420433396855117	25.168625254922937	25.29810660515084
29	24.937505789725527	24.723399127832263	25.18636821748891	25.1527268649533
30	24.953246617562556	24.779397168677846	25.37428825158021	24.893067962179387
31	25.58948941795625	24.381326903172873	24.556916531892398	25.472267146978478
32	25.448969584852883	24.49419529550083	24.378853435042195	25.67798168460409
33	24.907016590747627	24.259336648696838	25.027302993578225	25.806343766977307
34	25.691927827906962	24.4262346463658	25.00795759663169	24.873879929095548
35	25.97507344654805	24.191182513801266	25.15236066747554	24.681383372175148
36	24.762091160022816	24.87060670497911	24.881332888498537	25.48596924649954
37	25.90860334884239	24.509235784136184	24.731493605995595	24.850667261025826
38	25.089326633893833	24.360713832986708	25.089396284290633	25.460563248828826
39	25.851587694065042	24.176899556323264	24.574536995117466	25.39697575449423
40	25.76363006976885	24.453992746602626	24.92301884532099	24.859358338307537
41	24.8594964648621	24.975744029688055	25.229552373741843	24.935207131708005
42	26.009383719747742	24.808975271613832	24.743222366018006	24.438418642620423
43	25.564033893228665	24.094979051811507	24.914936075810502	25.426050979149327
44	25.027634969049533	24.696154647167443	24.706741957301098	25.56946842648193
45	25.225392765700178	24.855333506069446	24.769453011598394	25.149820716631982
46	25.10803002273436	24.63774071680114	24.822736036196673	25.43149322426782
47	25.275799887583556	24.565431037665082	25.143463304110163	25.0153057706412
48	25.866503956653386	25.06535217036073	24.080246126756393	24.987897746229496
49	25.2622963951006	25.1746030640426	24.23637497083274	25.326725570024067
50	25.32673410766972	25.26070470368957	24.35266109642243	25.059900092218278
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	118.5
2	40.0
3	115.0
4	190.0
5	203.0
6	216.0
7	168.0
8	120.0
9	202.0
10	284.0
11	436.0
12	588.0
13	1109.0
14	1630.0
15	2102.0
16	2574.0
17	2557.5
18	2541.0
19	2406.0
20	2271.0
21	2778.0
22	3285.0
23	4043.5
24	4802.0
25	6036.0
26	7270.0
27	9214.0
28	11158.0
29	13128.5
30	15099.0
31	17674.0
32	20249.0
33	23335.5
34	26422.0
35	30927.0
36	35432.0
37	41555.5
38	47679.0
39	53039.5
40	58400.0
41	64091.0
42	69782.0
43	73499.5
44	77217.0
45	85020.0
46	92823.0
47	98938.5
48	105054.0
49	109136.5
50	113219.0
51	111136.5
52	109054.0
53	109153.5
54	109253.0
55	109691.5
56	110130.0
57	106613.5
58	103097.0
59	94747.5
60	86398.0
61	76038.0
62	65678.0
63	56961.0
64	48244.0
65	40541.0
66	32838.0
67	28662.5
68	24487.0
69	21650.5
70	18814.0
71	14591.0
72	10368.0
73	9073.0
74	7778.0
75	5714.5
76	3651.0
77	3221.5
78	2792.0
79	2389.0
80	1986.0
81	1549.5
82	1113.0
83	968.0
84	823.0
85	623.0
86	423.0
87	286.0
88	149.0
89	100.5
90	52.0
91	36.5
92	21.0
93	16.5
94	12.0
95	14.0
96	16.0
97	13.5
98	11.0
99	11.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030924776178449888
2	0.005154129363074982
3	2.089511903949317E-4
4	6.965039679831056E-5
5	6.965039679831056E-5
6	2.089511903949317E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.358047615797268E-4
17	0.0
18	2.089511903949317E-4
19	0.0
20	4.875527775881739E-4
21	0.0
22	0.0
23	6.965039679831056E-5
24	0.0011840567455712796
25	2.089511903949317E-4
26	4.179023807898634E-4
27	2.7860158719324223E-4
28	9.751055551763478E-4
29	6.26853571184795E-4
30	0.0011840567455712796
31	5.572031743864845E-4
32	6.965039679831056E-4
33	1.3930079359662111E-4
34	4.875527775881739E-4
35	2.7860158719324223E-4
36	2.089511903949317E-4
37	0.0015323087295628323
38	0.0
39	8.358047615797268E-4
40	6.965039679831056E-5
41	9.054551583780374E-4
42	0.004457625395091876
43	0.003970072617503702
44	0.004248674204696944
45	0.0018109103167560747
46	0.0020895119039493165
47	6.26853571184795E-4
48	0.0041093734111003226
49	0.003970072617503702
50	0.00125370714236959
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1435742.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.69323433010561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86935864152785	44.14665796599173
2	13.37932848976973	15.170348104999462
3	3.8923802416573894	6.6201487542646635
4	1.5728073703116543	3.5667014720478325
5	0.8335624120804228	2.362867457842173
6	0.5355144024702863	1.8216026103836653
7	0.34878227240798265	1.3841516569868741
8	0.2492044146678725	1.1302563421490002
9	0.2062565613503999	1.0524016408284023
>10	0.9804819139122343	9.895424898673493
>50	0.07040713239383402	2.7963018830338644
>100	0.05400676808081334	6.393284735514154
>500	0.006302786685138702	2.473602337670131
>1k	0.0016065926844471203	1.1862501396145868
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1687	0.11750021939874991	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1513	0.10538105035584387	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1442	0.10043587218316383	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.965039679831056E-5
2	0.0	0.0	0.0	0.0	6.965039679831056E-5
3	0.0	0.0	0.0	0.0	6.965039679831056E-5
4	0.0	0.0	0.0	0.0	6.965039679831056E-5
5	0.0	0.0	0.0	0.0	6.965039679831056E-5
6	0.0	0.0	0.0	0.0	6.965039679831056E-5
7	0.0	0.0	0.0	0.0	6.965039679831056E-5
8	0.0	0.0	0.0	0.0	6.965039679831056E-5
9	0.0	0.0	0.0	0.0	6.965039679831056E-5
10	0.0	0.0	0.0	0.0	6.965039679831056E-5
11	0.0	0.0	0.0	0.0	6.965039679831056E-5
12	0.0	0.0	0.0	0.0	2.0895119039493167E-4
13	0.0	0.0	0.0	0.0	2.0895119039493167E-4
14	0.0	0.0	0.0	0.0	2.0895119039493167E-4
15	0.0	0.0	0.0	0.0	4.1790238078986334E-4
16	2.7860158719324223E-4	0.0	0.0	1.3930079359662111E-4	4.875527775881739E-4
17	2.7860158719324223E-4	0.0	0.0	2.0895119039493167E-4	4.875527775881739E-4
18	2.7860158719324223E-4	0.0	0.0	4.1790238078986334E-4	4.875527775881739E-4
19	2.7860158719324223E-4	0.0	0.0	4.875527775881739E-4	4.875527775881739E-4
20	2.7860158719324223E-4	0.0	0.0	4.875527775881739E-4	4.875527775881739E-4
21	2.7860158719324223E-4	0.0	0.0	5.572031743864845E-4	5.572031743864845E-4
22	2.7860158719324223E-4	0.0	0.0	8.358047615797267E-4	6.965039679831056E-4
23	2.7860158719324223E-4	0.0	0.0	0.00125370714236959	6.965039679831056E-4
24	2.7860158719324223E-4	0.0	0.0	0.0014626583327645218	6.965039679831056E-4
25	2.7860158719324223E-4	0.0	0.0	0.0019502111103526956	6.965039679831056E-4
26	2.7860158719324223E-4	0.0	0.0	0.002228812697545938	6.965039679831056E-4
27	2.7860158719324223E-4	0.0	0.0	0.00250741428473918	7.661543647814161E-4
28	2.7860158719324223E-4	0.0	0.0	0.002994967062327354	7.661543647814161E-4
29	2.7860158719324223E-4	0.0	0.0	0.004109373411100323	7.661543647814161E-4
30	2.7860158719324223E-4	0.0	0.0	0.006338186108646261	7.661543647814161E-4
31	2.7860158719324223E-4	0.0	0.0	0.011979868249309416	7.661543647814161E-4
32	2.7860158719324223E-4	0.0	0.0	0.01748224959637595	7.661543647814161E-4
33	2.7860158719324223E-4	0.0	0.0	0.02228812697545938	7.661543647814161E-4
34	2.7860158719324223E-4	0.0	0.0	0.029044215464895502	9.054551583780372E-4
35	2.7860158719324223E-4	0.0	0.0	0.0362878567319198	9.054551583780372E-4
36	2.7860158719324223E-4	0.0	0.0	0.0468050666484647	9.054551583780372E-4
37	2.7860158719324223E-4	0.0	0.0	0.06101374759532005	9.054551583780372E-4
38	2.7860158719324223E-4	0.0	0.0	0.08128201306362842	9.054551583780372E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1825	0.0	25.56203	1
GGTATCA	840	0.0	24.362734	1
ATACCGT	485	0.0	19.957783	6
TATACAA	190	0.0	19.683245	5
GTATAGG	185	1.8189894E-12	19.031395	1
ACCGTCG	490	0.0	18.856218	8
TACCGTC	515	0.0	18.36803	7
TCTATAC	120	3.1646232E-7	18.332434	3
TAGCCCG	75	0.0012914554	17.599136	5
GGCGTTA	415	0.0	17.496162	42
GTAGGAC	390	0.0	17.48632	3
CGTCGTA	530	0.0	17.433105	10
ACGACGG	470	0.0	17.3183	6
CGTATAT	305	0.0	17.310625	10
CGACGGT	445	0.0	17.302523	7
GTAATAC	140	8.379902E-8	17.284866	3
TAGCGTA	285	0.0	16.981623	7
TACGACG	480	0.0	16.9575	5
TCAACGC	2735	0.0	16.73044	4
GACGGTA	465	0.0	16.558329	8
>>END_MODULE