##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062041_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3250
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.454461538461537	32.0	32.0	32.0	32.0	32.0
2	31.18276923076923	32.0	32.0	32.0	32.0	32.0
3	31.198153846153847	32.0	32.0	32.0	32.0	32.0
4	31.29323076923077	32.0	32.0	32.0	32.0	32.0
5	31.304615384615385	32.0	32.0	32.0	32.0	32.0
6	34.90923076923077	36.0	36.0	36.0	36.0	36.0
7	34.86492307692308	36.0	36.0	36.0	36.0	36.0
8	34.83723076923077	36.0	36.0	36.0	36.0	36.0
9	34.90123076923077	36.0	36.0	36.0	36.0	36.0
10	34.74738461538462	36.0	36.0	36.0	32.0	36.0
11	34.696	36.0	36.0	36.0	32.0	36.0
12	34.71846153846154	36.0	36.0	36.0	32.0	36.0
13	34.82153846153846	36.0	36.0	36.0	32.0	36.0
14	34.643076923076926	36.0	36.0	36.0	32.0	36.0
15	34.73107692307692	36.0	36.0	36.0	32.0	36.0
16	34.64830769230769	36.0	36.0	36.0	32.0	36.0
17	34.71415384615385	36.0	36.0	36.0	32.0	36.0
18	34.60953846153846	36.0	36.0	36.0	32.0	36.0
19	34.57569230769231	36.0	36.0	36.0	32.0	36.0
20	34.65723076923077	36.0	36.0	36.0	32.0	36.0
21	34.643692307692305	36.0	36.0	36.0	32.0	36.0
22	34.60769230769231	36.0	36.0	36.0	32.0	36.0
23	34.58676923076923	36.0	36.0	36.0	32.0	36.0
24	34.561846153846155	36.0	36.0	36.0	32.0	36.0
25	34.11876923076923	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	0.0
7	0.0
8	1.0
9	0.0
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	2.0
17	2.0
18	0.0
19	1.0
20	1.0
21	2.0
22	8.0
23	11.0
24	12.0
25	10.0
26	27.0
27	31.0
28	28.0
29	58.0
30	72.0
31	110.0
32	194.0
33	267.0
34	618.0
35	1792.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.42874730686365	17.420744844567558	12.280701754385964	26.869806094182824
2	17.26153846153846	18.52307692307692	37.63076923076923	26.584615384615383
3	20.36923076923077	21.846153846153847	28.95384615384615	28.83076923076923
4	14.58910433979686	16.528162511542014	36.41120344721453	32.4715297014466
5	15.789473684210526	36.10341643582641	32.625423207140656	15.481686672822406
6	32.112068965517246	34.45197044334976	17.67241379310345	15.763546798029557
7	29.59309494451295	28.822441430332923	21.30086313193588	20.28360049321825
8	27.110289587184226	34.90449784349969	18.299445471349355	19.68576709796673
9	25.855161787365176	15.10015408320493	19.84591679506934	39.198767334360554
10	17.036352433764634	25.600739371534196	33.826247689463955	23.536660505237215
11	36.713933415536374	21.979038224414303	21.20838471023428	20.098643649815042
12	23.0958988590811	25.007708911501698	28.02960222016651	23.866790009250693
13	29.87053020961776	18.18742293464858	24.72256473489519	27.219482120838475
14	24.43006777572397	20.147874306839185	23.475046210720887	31.947011706715955
15	24.80739599383667	28.56702619414484	22.496147919876734	24.129429892141758
16	24.00616332819723	27.24191063174114	25.053929121725734	23.6979969183359
17	24.69173859432799	24.506781750924784	26.263871763255242	24.537607891491987
18	23.667180277349768	25.023112480739602	26.440677966101696	24.869029275808938
19	24.53004622496148	24.74576271186441	26.90292758089368	23.82126348228043
20	27.54158964879852	24.43006777572397	23.937153419593347	24.091189155884166
21	26.27040344933785	26.024022174314755	23.960578995996304	23.744995380351096
22	27.373612823674478	25.030826140567203	23.581997533908755	24.013563501849568
23	27.3959938366718	24.869029275808938	23.790446841294298	23.944530046224962
24	25.67026194144838	25.39291217257319	24.961479198767332	23.975346687211093
25	25.608628659476118	25.08474576271186	23.359013867488443	25.947611710323574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	3.0
9	3.0
10	4.5
11	6.0
12	6.0
13	6.0
14	12.0
15	18.0
16	18.0
17	18.0
18	21.5
19	25.0
20	25.0
21	25.0
22	45.5
23	66.0
24	66.0
25	66.0
26	89.0
27	112.0
28	112.0
29	112.0
30	139.0
31	166.0
32	166.0
33	166.0
34	207.5
35	249.0
36	249.0
37	249.0
38	261.0
39	273.0
40	273.0
41	273.0
42	294.0
43	315.0
44	315.0
45	315.0
46	323.5
47	332.0
48	332.0
49	332.0
50	364.0
51	396.0
52	396.0
53	396.0
54	368.0
55	340.0
56	340.0
57	340.0
58	321.5
59	303.0
60	303.0
61	303.0
62	268.5
63	234.0
64	234.0
65	234.0
66	202.0
67	170.0
68	170.0
69	170.0
70	145.0
71	120.0
72	120.0
73	120.0
74	94.0
75	68.0
76	68.0
77	68.0
78	48.0
79	28.0
80	28.0
81	28.0
82	21.5
83	15.0
84	15.0
85	15.0
86	11.0
87	7.0
88	7.0
89	7.0
90	4.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	1.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03076923076923077
2	0.0
3	0.0
4	0.03076923076923077
5	0.03076923076923077
6	0.06153846153846154
7	0.1846153846153846
8	0.12307692307692308
9	0.15384615384615385
10	0.12307692307692308
11	0.1846153846153846
12	0.21538461538461537
13	0.1846153846153846
14	0.12307692307692308
15	0.15384615384615385
16	0.15384615384615385
17	0.1846153846153846
18	0.15384615384615385
19	0.15384615384615385
20	0.12307692307692308
21	0.0923076923076923
22	0.1846153846153846
23	0.15384615384615385
24	0.15384615384615385
25	0.15384615384615385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3250.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.84615384615384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.75494071146245	65.2
2	10.118577075098814	15.753846153846155
3	3.517786561264822	8.215384615384616
4	1.3438735177865613	4.184615384615385
5	0.3952569169960474	1.5384615384615385
6	0.23715415019762848	1.1076923076923075
7	0.1976284584980237	1.0769230769230769
8	0.2766798418972332	1.723076923076923
9	0.11857707509881424	0.8307692307692307
>10	0.039525691699604744	0.3692307692307692
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTAGAGCTATCCAGAGTGCTCTC	12	0.3692307692307692	No Hit
TATATACACCACCCAACTAAGCCAC	9	0.2769230769230769	No Hit
GTCTTGATTTCTGTTAGTAGGATTC	9	0.2769230769230769	No Hit
GCACAGCAGCATCGTTTGCATTTTA	9	0.2769230769230769	No Hit
GTACATGGGAGGAGGAGAAATCGGA	8	0.24615384615384617	No Hit
CTGTTATAGTTGTCACTGTTAAGAG	8	0.24615384615384617	No Hit
GGATTAGAGTGATTGTGTTGATAAT	8	0.24615384615384617	No Hit
GCTGTATGCAGCATGTCCTGAAGCT	8	0.24615384615384617	No Hit
GTCCACCATCAGCAGAGGCAGGGGG	8	0.24615384615384617	No Hit
GTTGAGGGATGACGTTATTGAAGGA	8	0.24615384615384617	No Hit
GTATCAACGCAGAGTACATGGGAGG	8	0.24615384615384617	No Hit
GAGGAGGAGAAATCGGAATTGTGGG	7	0.21538461538461537	No Hit
GACTTGCATAGAGATTTCTGTGAGT	7	0.21538461538461537	No Hit
GGTATCAACGCAGAGTACATGGGGC	7	0.21538461538461537	No Hit
GGTATCAACGCAGAGTACATGGGAG	7	0.21538461538461537	No Hit
TATCAACGCAGAGTACATGGGAGGA	7	0.21538461538461537	No Hit
GTTCTAAGCAGTCGGCCACGAGGAC	6	0.1846153846153846	No Hit
GTTATTCACAACCCAACCATTTCGT	6	0.1846153846153846	No Hit
GTATCAACGCAGAGTACATGGGGCC	6	0.1846153846153846	No Hit
CTAATAAAGGCGTGTGGGAGAGATG	6	0.1846153846153846	No Hit
TCTGATGACTTAGAAATTTGTCGGA	6	0.1846153846153846	No Hit
CTCTGGCACTGTCCACCATCAGCAG	6	0.1846153846153846	No Hit
GTCCAATATGTAGTTGGTAAATCTG	5	0.15384615384615385	No Hit
GGCTAACAGAGTCACCTGAAGAGCA	5	0.15384615384615385	No Hit
GCTTATAGAGGCTAACAGAGTCACC	5	0.15384615384615385	No Hit
ACCCGGGCCTGACCCGGGGCACAAG	5	0.15384615384615385	No Hit
GACTAAGGAAAAAACATCCAAGACT	5	0.15384615384615385	No Hit
GTGCTGTGTTGAGGCAGGAAGAGAA	5	0.15384615384615385	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5	0.15384615384615385	No Hit
CTGTAGGACCTGGAATATGGCGAGA	5	0.15384615384615385	No Hit
GTTTATATGGACCTCTTGTATTTTA	5	0.15384615384615385	No Hit
ACTATGGGTCGACGGAGAGCAATTT	5	0.15384615384615385	No Hit
CTCTTAGACACTAACATTTCTCCGT	4	0.12307692307692308	No Hit
ACTTATGAAAGTCCTTCTTGATGTC	4	0.12307692307692308	No Hit
GCTTAGAACTGCCTGTGACTCCAGC	4	0.12307692307692308	No Hit
CCTCTGCACAGCACAGCAGCATCGT	4	0.12307692307692308	No Hit
ACGGAGAGCAATTTGCAAAGTTGAG	4	0.12307692307692308	No Hit
CTCACTACCAGCCCTTCCACTGCAG	4	0.12307692307692308	No Hit
CCGTGAGACTGCCCACACAGCCAGA	4	0.12307692307692308	No Hit
GAGTACGTCTGATGACTTAGAAATT	4	0.12307692307692308	No Hit
GTATAGCCTTGGCTGTCCTCAGCTC	4	0.12307692307692308	No Hit
GTCTAAGAGGAGCGAGGTTAACGAA	4	0.12307692307692308	No Hit
TTGCAGGGCAGGTACCCAGATAACT	4	0.12307692307692308	No Hit
CTCTTTAGCAGTGCTCTCCTGTCAT	4	0.12307692307692308	No Hit
GTGAATAACTAACGAAGGATTAGAG	4	0.12307692307692308	No Hit
AGCCTACACTGTGAACCAGACAGAA	4	0.12307692307692308	No Hit
TCGTAGTTGAGAGCACGCACTGTTC	4	0.12307692307692308	No Hit
GTCTTAGGCGAAAGACTTGCATAGA	4	0.12307692307692308	No Hit
GTATGTGGGATTTAATAACAACACG	4	0.12307692307692308	No Hit
GCGTAAGAACGCCAGCCTGGCTCTC	4	0.12307692307692308	No Hit
ACCTACAACTACGATCGCCACATCC	4	0.12307692307692308	No Hit
CCAGGGTACCTTGCCAGCAGACACG	4	0.12307692307692308	No Hit
TATATATACACCACCCAACTAAGCC	4	0.12307692307692308	No Hit
TGCTTATAGAGGCTAACAGAGTCAC	4	0.12307692307692308	No Hit
GGGGAAAACAGGTCAGGATCCGGTG	4	0.12307692307692308	No Hit
GGTATGCAGTGTTCCAGTGGGCAAC	4	0.12307692307692308	No Hit
TCCTACCACTGTGGAGGTGTTAGCC	4	0.12307692307692308	No Hit
GCATACAGCTGTGCACACACACAGA	4	0.12307692307692308	No Hit
GTGCCAGAGGCAGGACCTGCCTGAG	4	0.12307692307692308	No Hit
ATCAGAACCTTGCCTAACAAACATT	4	0.12307692307692308	No Hit
GCATAGAGATTTCTGTGAGTACGTC	4	0.12307692307692308	No Hit
GCTGTACATAGGGAAGCAGACTACC	4	0.12307692307692308	No Hit
GTGTATTAATGAGCTGGCTGAAGTG	4	0.12307692307692308	No Hit
GATCGAGACGGTCCATAAGATCGGA	4	0.12307692307692308	No Hit
CCTTTAATCCTAGCACTTAGGGAGG	4	0.12307692307692308	No Hit
GAGTATTCTCTGCAGGCAAGCTCTC	4	0.12307692307692308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE