##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3250
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.364307692307694	32.0	32.0	32.0	32.0	32.0
2	31.447692307692307	32.0	32.0	32.0	32.0	32.0
3	31.60676923076923	32.0	32.0	32.0	32.0	32.0
4	31.687076923076923	32.0	32.0	32.0	32.0	32.0
5	31.65230769230769	32.0	32.0	32.0	32.0	32.0
6	35.17661538461538	36.0	36.0	36.0	36.0	36.0
7	35.22123076923077	36.0	36.0	36.0	36.0	36.0
8	35.228	36.0	36.0	36.0	36.0	36.0
9	35.30523076923077	36.0	36.0	36.0	36.0	36.0
10	35.132	36.0	36.0	36.0	36.0	36.0
11	35.20923076923077	36.0	36.0	36.0	36.0	36.0
12	35.213846153846156	36.0	36.0	36.0	36.0	36.0
13	35.285538461538465	36.0	36.0	36.0	36.0	36.0
14	35.208	36.0	36.0	36.0	36.0	36.0
15	35.141230769230766	36.0	36.0	36.0	36.0	36.0
16	35.28246153846154	36.0	36.0	36.0	36.0	36.0
17	35.26092307692308	36.0	36.0	36.0	36.0	36.0
18	35.23323076923077	36.0	36.0	36.0	36.0	36.0
19	35.19107692307692	36.0	36.0	36.0	36.0	36.0
20	35.251384615384616	36.0	36.0	36.0	36.0	36.0
21	35.23907692307692	36.0	36.0	36.0	36.0	36.0
22	35.09723076923077	36.0	36.0	36.0	36.0	36.0
23	35.06307692307692	36.0	36.0	36.0	36.0	36.0
24	35.15938461538462	36.0	36.0	36.0	36.0	36.0
25	35.10953846153846	36.0	36.0	36.0	36.0	36.0
26	35.08953846153846	36.0	36.0	36.0	36.0	36.0
27	35.108	36.0	36.0	36.0	36.0	36.0
28	35.03907692307693	36.0	36.0	36.0	36.0	36.0
29	34.972923076923074	36.0	36.0	36.0	36.0	36.0
30	34.89723076923077	36.0	36.0	36.0	32.0	36.0
31	34.95569230769231	36.0	36.0	36.0	36.0	36.0
32	34.85569230769231	36.0	36.0	36.0	32.0	36.0
33	34.823076923076925	36.0	36.0	36.0	32.0	36.0
34	34.83846153846154	36.0	36.0	36.0	32.0	36.0
35	34.725846153846156	36.0	36.0	36.0	32.0	36.0
36	34.76676923076923	36.0	36.0	36.0	32.0	36.0
37	34.81138461538462	36.0	36.0	36.0	32.0	36.0
38	34.705230769230766	36.0	36.0	36.0	32.0	36.0
39	34.77969230769231	36.0	36.0	36.0	32.0	36.0
40	34.65476923076923	36.0	36.0	36.0	32.0	36.0
41	34.74246153846154	36.0	36.0	36.0	32.0	36.0
42	34.796	36.0	36.0	36.0	32.0	36.0
43	34.808615384615386	36.0	36.0	36.0	32.0	36.0
44	34.677846153846154	36.0	36.0	36.0	32.0	36.0
45	34.62276923076923	36.0	36.0	36.0	32.0	36.0
46	34.58123076923077	36.0	36.0	36.0	32.0	36.0
47	34.63415384615384	36.0	36.0	36.0	32.0	36.0
48	34.58953846153846	36.0	36.0	36.0	32.0	36.0
49	34.60153846153846	36.0	36.0	36.0	32.0	36.0
50	34.103692307692306	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	2.0
24	1.0
25	3.0
26	10.0
27	18.0
28	35.0
29	34.0
30	53.0
31	80.0
32	154.0
33	230.0
34	561.0
35	2069.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.825069294733595	19.15614413304589	12.442254388666461	24.57653218355405
2	16.76923076923077	21.29230769230769	35.63076923076923	26.30769230769231
3	19.015384615384615	24.646153846153844	27.723076923076924	28.615384615384613
4	14.03076923076923	17.507692307692306	36.769230769230774	31.692307692307693
5	16.184615384615384	36.52307692307693	33.10769230769231	14.184615384615384
6	31.969230769230773	34.676923076923075	18.06153846153846	15.292307692307691
7	29.323076923076925	30.092307692307692	20.36923076923077	20.215384615384615
8	28.06153846153846	32.30769230769231	18.553846153846155	21.076923076923077
9	26.8	15.784615384615385	19.015384615384615	38.4
10	16.0	26.123076923076923	31.784615384615382	26.092307692307692
11	36.89230769230769	23.507692307692306	21.846153846153847	17.753846153846155
12	23.29230769230769	23.384615384615383	27.846153846153847	25.476923076923075
13	31.169230769230772	19.29230769230769	24.123076923076923	25.415384615384617
14	23.29230769230769	20.43076923076923	24.123076923076923	32.15384615384615
15	24.52307692307692	28.307692307692307	21.661538461538463	25.50769230769231
16	25.91566635887966	25.176977531548168	24.684518313327178	24.222837796245
17	23.661538461538463	26.769230769230766	24.83076923076923	24.73846153846154
18	23.446153846153848	26.123076923076923	25.784615384615385	24.646153846153844
19	25.538461538461537	25.538461538461537	24.861538461538462	24.06153846153846
20	24.953846153846154	26.553846153846155	23.692307692307693	24.8
21	26.584615384615383	25.29230769230769	24.123076923076923	24.0
22	25.846153846153847	24.4	24.73846153846154	25.01538461538462
23	24.184615384615384	25.261538461538464	24.276923076923076	26.276923076923076
24	23.815384615384616	24.676923076923078	25.50769230769231	26.0
25	24.553846153846155	23.56923076923077	25.63076923076923	26.246153846153845
26	24.43076923076923	24.73846153846154	25.63076923076923	25.2
27	24.73846153846154	24.43076923076923	25.323076923076922	25.50769230769231
28	23.93846153846154	26.338461538461537	25.353846153846156	24.369230769230768
29	25.01538461538462	25.415384615384617	24.8	24.76923076923077
30	26.553846153846155	24.676923076923078	25.384615384615383	23.384615384615383
31	27.692307692307693	24.184615384615384	24.153846153846153	23.96923076923077
32	23.815384615384616	25.323076923076922	25.72307692307692	25.138461538461538
33	24.461538461538463	26.73846153846154	25.29230769230769	23.507692307692306
34	25.29230769230769	25.815384615384616	24.953846153846154	23.93846153846154
35	26.21538461538461	23.26153846153846	25.784615384615385	24.73846153846154
36	24.676923076923078	23.507692307692306	26.03076923076923	25.784615384615385
37	25.815384615384616	22.400000000000002	25.261538461538464	26.52307692307692
38	24.246153846153845	26.646153846153847	25.63076923076923	23.476923076923075
39	26.30769230769231	23.415384615384617	24.73846153846154	25.538461538461537
40	24.43076923076923	24.923076923076923	25.907692307692308	24.73846153846154
41	27.384615384615387	23.107692307692307	25.29230769230769	24.215384615384615
42	24.038165589412127	24.7460757156048	26.500461680517084	24.71529701446599
43	23.692307692307693	24.8	25.692307692307693	25.815384615384616
44	23.96923076923077	24.70769230769231	24.4	26.923076923076923
45	24.49230769230769	23.876923076923077	25.784615384615385	25.846153846153847
46	25.63076923076923	23.846153846153847	24.184615384615384	26.338461538461537
47	23.63076923076923	24.584615384615386	25.63076923076923	26.153846153846157
48	24.007386888273317	25.361649738381043	23.976608187134502	26.65435518621114
49	25.353846153846156	24.892307692307693	24.584615384615386	25.169230769230772
50	22.553846153846155	25.815384615384616	27.10769230769231	24.52307692307692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.5
14	2.0
15	2.5
16	3.0
17	3.0
18	3.0
19	2.5
20	2.0
21	4.5
22	7.0
23	17.0
24	27.0
25	29.5
26	32.0
27	39.0
28	46.0
29	52.5
30	59.0
31	71.5
32	84.0
33	92.0
34	100.0
35	110.0
36	120.0
37	129.0
38	138.0
39	172.0
40	206.0
41	215.5
42	225.0
43	206.5
44	188.0
45	178.0
46	168.0
47	159.0
48	150.0
49	159.0
50	168.0
51	172.0
52	176.0
53	175.5
54	175.0
55	179.0
56	183.0
57	163.0
58	143.0
59	159.0
60	175.0
61	152.5
62	130.0
63	126.0
64	122.0
65	112.5
66	103.0
67	101.5
68	100.0
69	83.5
70	67.0
71	60.0
72	53.0
73	49.0
74	45.0
75	34.5
76	24.0
77	15.0
78	6.0
79	7.5
80	9.0
81	6.5
82	4.0
83	5.0
84	6.0
85	3.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0923076923076923
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.03076923076923077
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.03076923076923077
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.03076923076923077
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3250.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.46153846153847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.05882352941177	65.16923076923077
2	11.568627450980392	18.153846153846153
3	2.823529411764706	6.646153846153846
4	1.0980392156862746	3.446153846153846
5	0.6274509803921569	2.4615384615384617
6	0.5882352941176471	2.769230769230769
7	0.19607843137254902	1.0769230769230769
8	0.0	0.0
9	0.0392156862745098	0.2769230769230769
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGATTAGAGTGATTGTGTTGATAATTGATTGGATTTGGTACTCTACCCGA	9	0.2769230769230769	No Hit
CCCACATACACCGCCACATCACTTTCGTTCTCAAATCACTTGGCTTAAAA	7	0.21538461538461537	No Hit
GTCTTAGGCGAAAGACTTGCATAGAGATTTCTGTGAGTACGTCTGATGAC	7	0.21538461538461537	No Hit
GCTTATAGAGGCTAACAGAGTCACCTGAAGAGCAAGCTCTTAACAGTGAC	7	0.21538461538461537	No Hit
ATATTGGACTTGGGAGGTGAAAAACCAAAGGTTCAACTTGTTTCAAACTT	7	0.21538461538461537	No Hit
CTATAACAGCTAGCTTCAGAGATTACCAGATGGCGAAAGGCAAACGTAAG	7	0.21538461538461537	No Hit
GTCTTGATTTCTGTTAGTAGGATTCTTACGTTTGCCTTTCGCCATCTGGT	6	0.1846153846153846	No Hit
TTCCAGAGGGGCGGATCAACTATGGGTCGACGGAGAGCAATTTGCAAAGT	6	0.1846153846153846	No Hit
CTATGTAGTTGTGGCTAGCCTTGAACTTCCATGTTGACCAGGCTGGCTTC	6	0.1846153846153846	No Hit
CTCTAATCCTTCGTTAGTTATTCACAACCCAACCATTTCGTTAACCTCGC	6	0.1846153846153846	No Hit
GTTGAGGGATGACGTTATTGAAGGACGGAGAAATGTTAGTGTCTAAGAGG	6	0.1846153846153846	No Hit
ACTATGGGTCGACGGAGAGCAATTTGCAAAGTTGAGGGATGACGTTATTG	6	0.1846153846153846	No Hit
GTATCAACGCAGAGTACATGGGAGGAGGAGAAATCGGAATTGTGGGAAAG	6	0.1846153846153846	No Hit
ATCCAGAGTGCTCTCGCTCGTGTTGTTATTAAATCCCACATACACCGCCA	6	0.1846153846153846	No Hit
GCATACAGCTGTGCACACACACAGATAAGATAAAGGAAAACATCTAGTCA	6	0.1846153846153846	No Hit
ATATATAGTCACACACACACACACACAACTTCTACAAACCCTAGTGCCCA	6	0.1846153846153846	No Hit
AAACTAAGGAGAGAGCTACCCTCCCAGGTCTGCTTATAGAGGCTAACAGA	6	0.1846153846153846	No Hit
GAGTATTCTCTGCAGGCAAGCTCTCTTCTTGCAGGGCAGGTACCCAGATA	6	0.1846153846153846	No Hit
ACCCGGGCCTGACCCGGGGCACAAGCCCCTTCCGCTCCACTCGAGCCCCA	6	0.1846153846153846	No Hit
GTACATGGGAGGAGGAGAAATCGGAATTGTGGGAAAGGAATGTTTTCAGT	6	0.1846153846153846	No Hit
GGATTCCACACGGGATCCTAAGACCACTAGTGAGTGGAACACAACTTCTG	6	0.1846153846153846	No Hit
GTATAATGCTGTCAGGTCAACTTAAACAAGCCAACAGAGGCTTTGAGCCT	5	0.15384615384615385	No Hit
ACCTACAACTACGATCGCCACATCCATAGTCGATGCAAAACTAGACTACT	5	0.15384615384615385	No Hit
TCCTAAGACCACTAGTGAGTGGAACACAACTTCTGCCAGGAGTCTGGTTC	5	0.15384615384615385	No Hit
GTGTTGGGGTGCCCAGGACTTGGTGGGGTGGGAGTGCTGTGTTCTGACGA	5	0.15384615384615385	No Hit
GTACATAGGGAAGCAGACTACCCGGGCCTGACCCGGGGCACAAGCCCCTT	5	0.15384615384615385	No Hit
ACTTAGAAATTTGTCGGAATTCTGGCAACAGTTAATTCCAGAGGGGCGGA	5	0.15384615384615385	No Hit
CTACCAGCCCTTCCACTGCAGGGGTGATGGTGTGCCCATGACTAGGATAA	5	0.15384615384615385	No Hit
GCTTAGAACTGCCTGTGACTCCAGCTTCAGGACATGCTGCATACAGCTGT	5	0.15384615384615385	No Hit
TATATACACCACCCAACTAAGCCACTCTAGAGCTATCCAGAGTGCTCTCG	5	0.15384615384615385	No Hit
CTCTAGAGCTATCCAGAGTGCTCTCGCTCGTGTTGTTATTAAATCCCACA	5	0.15384615384615385	No Hit
CTCACTACCAGCCCTTCCACTGCAGGGGTGATGGTGTGCCCATGACTAGG	5	0.15384615384615385	No Hit
TTCCAAGACTCCTCCCATTTCATGTTCAACACTCACTACCAGCCCTTCCA	5	0.15384615384615385	No Hit
TCCCAGGTCTGCTTATAGAGGCTAACAGAGTCACCTGAAGAGCAAGCTCT	5	0.15384615384615385	No Hit
GTCTAAGAGGAGCGAGGTTAACGAAATGGTTGGGTTGTGAATAACTAACG	5	0.15384615384615385	No Hit
ACCCATAGTTGATCCGCCCCTCTGGAATTAACTGTTGCCAGAATTCCGAC	5	0.15384615384615385	No Hit
GATTAAAGGCATGTATTCCCTACCCTCCATCATGCCTAATTTGCTCTTTT	5	0.15384615384615385	No Hit
TTCCAAGGGAGCACCAGGCCTGGAGGAAAACAGCTTGCTCAGGCAGGTCC	4	0.12307692307692308	No Hit
CTCTTAGACACTAACATTTCTCCGTCCTTCAATAACGTCATCCCTCAACT	4	0.12307692307692308	No Hit
GTATAGCCTTGGCTGTCCTCAGCTCACTCTGTAGACCAGGCTGGCCTCGA	4	0.12307692307692308	No Hit
TATATATACACCACCCAACTAAGCCACTCTAGAGCTATCCAGAGTGCTCT	4	0.12307692307692308	No Hit
ACCTGAGACTGCTGTACATAGGGAAGCAGACTACCCGGGCCTGACCCGGG	4	0.12307692307692308	No Hit
GTGTTGATAATTGATTGGATTTGGTACTCTACCCGATCAGGTATTTTAAG	4	0.12307692307692308	No Hit
GACCTGGGAGGGTAGCTCTCTCCTTAGTTTTGAGTGGGCAGAGTATTCTC	4	0.12307692307692308	No Hit
CCTCAACACAGCACTCAACGACATTGCTGTGGGGACATGGATCCAGGAGA	4	0.12307692307692308	No Hit
GCTCTTAACAGTGACAACTATAACAGCTAGCTTCAGAGATTACCAGATGG	4	0.12307692307692308	No Hit
CTTCAATAACGTCATCCCTCAACTTTGCAAATTGCTCTCCGTCGACCCAT	4	0.12307692307692308	No Hit
TCCTACCACTGTGGAGGTGTTAGCCGCGCTGGCTGCGGCTCTGCCTCCCT	4	0.12307692307692308	No Hit
TCTCTGAAGCTAGCTGTTATAGTTGTCACTGTTAAGAGCTTGCTCTTCAG	4	0.12307692307692308	No Hit
ATATATACACCACCCAACTAAGCCACTCTAGAGCTATCCAGAGTGCTCTC	4	0.12307692307692308	No Hit
TTCTTTAAGTGACTTATGAAAGTCCTTCTTGATGTCCTCTATCATCATCA	4	0.12307692307692308	No Hit
CCCCTGCAGTGGAAGGGCTGGTAGTGAGTGTTGAACATGAAATGGGAGGA	4	0.12307692307692308	No Hit
CTAGTCACCTGGGTGAACGCATCCTTCAACCACAGTCCCCCTGCCTCTGC	4	0.12307692307692308	No Hit
GGCTAACAGAGTCACCTGAAGAGCAAGCTCTTAACAGTGACAACTATAAC	4	0.12307692307692308	No Hit
GTGTATATATATTCAGTAACGTGAATTGCAGTAGTCTAGTTTTGCATCGA	4	0.12307692307692308	No Hit
CTCTTTAGCAGTGCTCTCCTGTCATTCTTTAAGTGACTTATGAAAGTCCT	4	0.12307692307692308	No Hit
AACTATAACAGCTAGCTTCAGAGATTACCAGATGGCGAAAGGCAAACGTA	4	0.12307692307692308	No Hit
CCTAAGACCACTAGTGAGTGGAACACAACTTCTGCCAGGAGTCTGGTTCG	4	0.12307692307692308	No Hit
CTTCCGCTCCACTCGAGCCCCAGGGTACCTTGCCAGCAGACACGCCCGAC	4	0.12307692307692308	No Hit
CTGCAAGAACAGTGCGTGCTCTCAACTACGAGATGGTCACGAGATGGTCA	4	0.12307692307692308	No Hit
CCTCAGTTGGGCTCATTTTGGTTGAAAAGAGTAGAAAATTACTGGTTTTA	4	0.12307692307692308	No Hit
GATTTGGTACTCTACCCGATCAGGTATTTTAAGCCAAGTGATTTGAGAAC	4	0.12307692307692308	No Hit
GAGATTCACACTGTGAACTTTTTTTCCAAGAGGCAAGACAGGAACTTAGA	4	0.12307692307692308	No Hit
GCCCAGGACTTGGTGGGGTGGGAGTGCTGTGTTCTGACGATGGTGAGTGG	4	0.12307692307692308	No Hit
CAAATCACTTGGCTTAAAATACCTGATCGGGTAGAGTACCAAATCCAATC	4	0.12307692307692308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.03076923076923077	0.0
30	0.0	0.0	0.0	0.03076923076923077	0.0
31	0.0	0.0	0.0	0.06153846153846154	0.0
32	0.0	0.0	0.0	0.09230769230769231	0.0
33	0.0	0.0	0.0	0.09230769230769231	0.0
34	0.0	0.0	0.0	0.15384615384615385	0.0
35	0.0	0.0	0.0	0.2153846153846154	0.0
36	0.0	0.0	0.0	0.27692307692307694	0.0
37	0.0	0.0	0.0	0.46153846153846156	0.0
38	0.0	0.0	0.0	0.5538461538461539	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE