FastQCFastQC Report
Thu 2 Feb 2017
SRR4062040_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062040_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449744
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTAT16960.3771034188338255No Hit
GTCCTAAAGTGTGTATTTCTCATTT15180.3375253477533886No Hit
CTTTAGGACGTGAAATATGGCGAGG14500.32240563520580595No Hit
GATATACACTGTTCTACAAATCCCG12840.28549574869258953No Hit
GTCCTACAGTGGACATTTCTAAATT12670.28171582055569394No Hit
GTCCTACAGTGTGCATTTCTCATTT12640.2810487744138888No Hit
CTGTAGGACGTGGAATATGGCAAGA12240.27215482585648726No Hit
GTATCAACGCAGAGTACTTTTTTTT10670.23724607776868617No Hit
TTGTAGAACAGTGTATATCAATGAG10360.23035326763669997No Hit
GTATTAGAGGCACTGCCTGCCCAGT9900.22012522679568822No Hit
CTGTAGGACCTGGAATATGGCGAGA9770.2172346935145327No Hit
GTGTATATCAATGAGTTACAATGAG8660.19255398626774342No Hit
GATTAAACCTTGTACCTTTTGCATA8390.18655057099149738No Hit
GTCAGGATACCGCGGCCGTTAAACT8150.18121420185705647No Hit
CTCTAATACTTGTAATGCTAGAGGT7990.17765662243409583No Hit
ACCTATAACTTCTCTGTTAACCCAA7930.1763225301504856No Hit
ATAAATAATCCACCTATAACTTCTC7650.17009676616030453No Hit
CCATTGGGATGTCCTGATCCAACAT7600.16898502259062934No Hit
GTTATATAATTTAAGCTCCATAGGG7490.1665391867373439No Hit
TATCAACGCAGAGTACTTTTTTTTT7200.1600910740332278No Hit
GCCTAAAGGAAAGATCCAAAAAGAT7170.15942402789142268No Hit
CCTATAACTTCTCTGTTAACCCAAC7060.15697819203813726No Hit
CACTATAAATAATCCACCTATAACT6940.15431000747091678No Hit
CTATAGAACTAGTACCGCAAGGGAA6910.15364296132911168No Hit
GACTATAGGCAATAATCACACTATA6790.15097477676189122No Hit
CTGTTAGTATGAGTAACAAGAATTC6760.1503077306200861No Hit
CTTCTACACCATTGGGATGTCCTGA6710.1491959870504109No Hit
ATCTATAACTTTATAGATGCAACAC6670.14830659219467077No Hit
GTTCATGCTAGTCCCTAATTAAGGA6510.14474901277171012No Hit
GTTATAGATTAACCCAATTTTAAGT6420.14274787434629477No Hit
CTGTTAACCCAACACCGGAATGCCT6380.1418584794905546No Hit
CCTCTAGCATTACAAGTATTAGAGG6280.13963499235120422No Hit
GTACATGGGAGAAATCGTAAATAGA6130.13629976164217866No Hit
GTGTATATCAATGAGTTACAATGAA6100.13563271550037354No Hit
GTATCAACGCAGAGTACATGGGAGA5990.1331868796470881No Hit
ATCCTGACCGTGCAAAGGTAGCATA5810.12918460279625743No Hit
GATTAAAGATAAGAGACAGTTGGAC5660.12584937208723185No Hit
GGTCAGGATACCGCGGCCGTTAAAC5620.1249599772314917No Hit
CTATTAAAGGTTTTTTCCGTTCCAG5490.12206944395033618No Hit
CTTTATTGGTGGCTGCTTTTAGGCC5420.12051300295279092No Hit
GTTCTACAAATCCCGTTTCCAACGA5340.11873421324131062No Hit
GTTCTACAGTGTGGTTTTTATCATT5200.11562133124622008No Hit
GTCCTTCAGTGTGCATTTCTCATTT5100.11339784410686969No Hit
ATTTAGAAATGTCCACTGTAGGACG5040.11206375182325945No Hit
TACTAACAGTGTTGCATCTATAAAG5030.11184140310932442No Hit
GCTATCACCAAGCTCGTTAGGCTTT5000.1111743569675193No Hit
GTATCCTGACCGTGCAAAGGTAGCA4920.109395567256039No Hit
GATATACACTGTTCTACAATGCCGG4900.10895086982816891No Hit
CTGAAGGACCTGGAATATGGCGAGA4870.10828382368636379No Hit
CATTTACACCTACTACCCAACTATC4790.10650503397488349No Hit
TGCTAGAGGTGATGTTTTTGGTAAA4780.10628268526094846No Hit
GATATATTTTGATCAACGGACCAAG4770.10606033654701343No Hit
ATCGTAAATAGATAGAAACCGACCT4740.1053932904052083No Hit
GTTAGTATGAGTAACAAGAATTCCA4730.10517094169127325No Hit
ACCTTTGCACGGTCAGGATACCGCG4710.10472624426340318No Hit
GTTTAAAATTGAACTTAAATTCATT4610.1025027571240528No Hit
GTATTGGAATTAGTGAAATTGGAGT4550.10116866484044255No Hit
GATAGAAACCGACCTGGATTGCTCC4540.10094631612650752No Hit
GAATATGGCAAGAAAACTGAAAATC4530.10072396741257249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCC307.702848E-419.00300613
TACGACC307.708619E-419.0008934
TTAGCCT359.916062E-519.000894
GGTCTAC250.00603554918.9945491
AAACTTG504.54572E-617.1008016
TAGCCTA504.54572E-617.1008015
AACTTGC402.7546522E-416.6257787
CGTCCCC350.002164681216.28829214
ACTGTGT508.688502E-515.2007128
CACCTTT1203.6379788E-1214.252254514
GTCCCCA400.00526584414.252254515
CCACCTT1203.6379788E-1214.252254513
TTGAGAT400.005269735614.2506689
GTTCGCA400.005269735614.2506689
CGTGTGC400.005269735614.2506688
CTCTGCC400.005269735614.2506689
ATAAAGG400.005269735614.2506683
TATACCA400.005269735614.2506685
GGGATGC400.005269735614.2506687
GTACGAC400.005269735614.2506683
Produced by FastQC (version 0.11.5)