##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062040_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449744
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.342783894837954	32.0	32.0	32.0	32.0	32.0
2	31.0585332989434	32.0	32.0	32.0	32.0	32.0
3	31.085070617951544	32.0	32.0	32.0	32.0	32.0
4	31.074073250560318	32.0	32.0	32.0	32.0	32.0
5	31.094075740865915	32.0	32.0	32.0	32.0	32.0
6	34.71257204098332	36.0	36.0	36.0	32.0	36.0
7	34.62715455903803	36.0	36.0	36.0	32.0	36.0
8	34.58684718417589	36.0	36.0	36.0	32.0	36.0
9	34.6580721477107	36.0	36.0	36.0	32.0	36.0
10	34.487417286278415	36.0	36.0	36.0	32.0	36.0
11	34.65872585292966	36.0	36.0	36.0	32.0	36.0
12	34.54158810345441	36.0	36.0	36.0	32.0	36.0
13	34.6040480806859	36.0	36.0	36.0	32.0	36.0
14	34.57703715891707	36.0	36.0	36.0	32.0	36.0
15	34.53609164324593	36.0	36.0	36.0	32.0	36.0
16	34.552074068803584	36.0	36.0	36.0	32.0	36.0
17	34.51664502472518	36.0	36.0	36.0	32.0	36.0
18	34.530137144686755	36.0	36.0	36.0	32.0	36.0
19	34.44667188444982	36.0	36.0	36.0	32.0	36.0
20	34.43623705930485	36.0	36.0	36.0	32.0	36.0
21	34.39140711160127	36.0	36.0	36.0	32.0	36.0
22	34.36641066918069	36.0	36.0	36.0	32.0	36.0
23	34.35605588957274	36.0	36.0	36.0	32.0	36.0
24	34.30502685972464	36.0	36.0	36.0	32.0	36.0
25	33.99674703831513	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	7.0
5	28.0
6	95.0
7	14.0
8	79.0
9	78.0
10	31.0
11	6.0
12	12.0
13	19.0
14	92.0
15	98.0
16	157.0
17	210.0
18	265.0
19	404.0
20	536.0
21	705.0
22	1009.0
23	1288.0
24	1787.0
25	2444.0
26	3492.0
27	4633.0
28	6350.0
29	8845.0
30	12476.0
31	17417.0
32	26393.0
33	39974.0
34	97191.0
35	223609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.65708923862338	22.404822363242598	13.180118202153254	24.75797019598077
2	13.114127147888578	22.19440392756768	43.639047991746416	21.052420932797325
3	16.999524171636182	27.60849553744147	32.071498770406144	23.320481520516207
4	9.822282311397325	19.138229316302795	43.33669852480087	27.70278984749901
5	10.099844083976683	41.435183640605786	37.88603673034535	10.578935545072186
6	26.482778874113695	43.39487893707576	19.223230554622372	10.899111634188179
7	24.92977559351309	34.275742642574464	23.264867319275098	17.52961444463735
8	22.518118845379917	40.152162686753215	20.743406877147997	16.58631159071888
9	24.893707009772275	16.041003494868995	22.54947354361908	36.51581595173965
10	13.859679979695386	28.52931812565261	36.34325483848704	21.267747056164964
11	31.339169620433537	24.250351780275366	28.076073597777107	16.33440500151399
12	21.807762182958108	27.672369264747793	32.96901717013394	17.55085138216016
13	26.09382896350898	23.22370369051091	28.999797408160223	21.68266993781989
14	18.82658819286924	25.994964795864988	28.58290835349715	26.595538657768618
15	21.78670822665034	34.150283869531336	24.13714794612045	19.925859957697874
16	20.692011789533673	29.209816300544063	29.992075011353215	20.10609689856905
17	17.86660433224248	30.938578329882677	29.966383935528395	21.228433402346447
18	17.74316692969823	30.07330010751276	33.08989001644967	19.093642946339333
19	22.154263902335884	28.503321870931124	28.543607206846282	20.798807019886713
20	20.614202002532654	31.510401018402916	29.506533045794747	18.368863933269683
21	21.09820800655195	28.036507526728567	28.12975706159365	22.73552740512583
22	20.841781942419622	31.093626539946804	28.481476123173486	19.583115394460087
23	20.84229031755569	30.66381823441707	29.05133854062354	19.442552907403698
24	21.76353419017415	30.29344016024036	29.093863016747346	18.849162632838144
25	20.837719405615804	30.063715813672975	30.66571118910221	18.432853591609007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	262.0
1	262.0
2	251.0
3	240.0
4	240.0
5	240.0
6	818.0
7	1396.0
8	1396.0
9	1396.0
10	1519.5
11	1643.0
12	1643.0
13	1643.0
14	2428.0
15	3213.0
16	3213.0
17	3213.0
18	5967.5
19	8722.0
20	8722.0
21	8722.0
22	12840.5
23	16959.0
24	16959.0
25	16959.0
26	26582.5
27	36206.0
28	36206.0
29	36206.0
30	46313.5
31	56421.0
32	56421.0
33	56421.0
34	57997.5
35	59574.0
36	59574.0
37	59574.0
38	59094.5
39	58615.0
40	58615.0
41	58615.0
42	54867.0
43	51119.0
44	51119.0
45	51119.0
46	52446.0
47	53773.0
48	53773.0
49	53773.0
50	46785.5
51	39798.0
52	39798.0
53	39798.0
54	32467.0
55	25136.0
56	25136.0
57	25136.0
58	20394.5
59	15653.0
60	15653.0
61	15653.0
62	12764.0
63	9875.0
64	9875.0
65	9875.0
66	7832.5
67	5790.0
68	5790.0
69	5790.0
70	4290.5
71	2791.0
72	2791.0
73	2791.0
74	2144.0
75	1497.0
76	1497.0
77	1497.0
78	1059.0
79	621.0
80	621.0
81	621.0
82	396.0
83	171.0
84	171.0
85	171.0
86	124.5
87	78.0
88	78.0
89	78.0
90	51.0
91	24.0
92	24.0
93	24.0
94	20.5
95	17.0
96	17.0
97	17.0
98	83.5
99	150.0
100	150.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013340922836102315
2	0.0
3	4.4469742787007723E-4
4	0.00933864598527162
5	0.03179586609271052
6	0.05936710662065531
7	0.10450389554946815
8	0.10850617240029885
9	0.11428723896260984
10	0.13007399765199756
11	0.13340922836102317
12	0.13163043864954285
13	0.12562702337329681
14	0.11273079796506456
15	0.13207513607741292
16	0.11828951581344054
17	0.12318118752001138
18	0.11006261339784411
19	0.09983457255683234
20	0.09183001885517095
21	0.0916076701412359
22	0.1020580596961827
23	0.08315841901170444
24	0.09316411113878117
25	0.09005122914369063
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	449744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.099309463202474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.22441676142419	15.86889201925471
2	14.89230148885889	7.475729673763603
3	6.68934649314481	5.036939332140893
4	3.6568755349851814	3.671402028840286
5	2.220048527247018	2.786084250434989
6	1.4845803348057633	2.2357164747764755
7	1.0996659655454728	1.9320599462764014
8	0.8123309555400134	1.6311156831710212
9	0.6198897228258544	1.4002923587638432
>10	4.33035928027416	21.860005535278237
>50	0.5480569378843472	9.745285676316758
>100	0.3822209388805075	18.313978687040112
>500	0.03192564686704935	5.414641162168961
>1k	0.007981411716762338	2.627857171773689
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTAT	1696	0.3771034188338255	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1518	0.3375253477533886	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1450	0.32240563520580595	No Hit
GATATACACTGTTCTACAAATCCCG	1284	0.28549574869258953	No Hit
GTCCTACAGTGGACATTTCTAAATT	1267	0.28171582055569394	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1264	0.2810487744138888	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1224	0.27215482585648726	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1067	0.23724607776868617	No Hit
TTGTAGAACAGTGTATATCAATGAG	1036	0.23035326763669997	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	990	0.22012522679568822	No Hit
CTGTAGGACCTGGAATATGGCGAGA	977	0.2172346935145327	No Hit
GTGTATATCAATGAGTTACAATGAG	866	0.19255398626774342	No Hit
GATTAAACCTTGTACCTTTTGCATA	839	0.18655057099149738	No Hit
GTCAGGATACCGCGGCCGTTAAACT	815	0.18121420185705647	No Hit
CTCTAATACTTGTAATGCTAGAGGT	799	0.17765662243409583	No Hit
ACCTATAACTTCTCTGTTAACCCAA	793	0.1763225301504856	No Hit
ATAAATAATCCACCTATAACTTCTC	765	0.17009676616030453	No Hit
CCATTGGGATGTCCTGATCCAACAT	760	0.16898502259062934	No Hit
GTTATATAATTTAAGCTCCATAGGG	749	0.1665391867373439	No Hit
TATCAACGCAGAGTACTTTTTTTTT	720	0.1600910740332278	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	717	0.15942402789142268	No Hit
CCTATAACTTCTCTGTTAACCCAAC	706	0.15697819203813726	No Hit
CACTATAAATAATCCACCTATAACT	694	0.15431000747091678	No Hit
CTATAGAACTAGTACCGCAAGGGAA	691	0.15364296132911168	No Hit
GACTATAGGCAATAATCACACTATA	679	0.15097477676189122	No Hit
CTGTTAGTATGAGTAACAAGAATTC	676	0.1503077306200861	No Hit
CTTCTACACCATTGGGATGTCCTGA	671	0.1491959870504109	No Hit
ATCTATAACTTTATAGATGCAACAC	667	0.14830659219467077	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	651	0.14474901277171012	No Hit
GTTATAGATTAACCCAATTTTAAGT	642	0.14274787434629477	No Hit
CTGTTAACCCAACACCGGAATGCCT	638	0.1418584794905546	No Hit
CCTCTAGCATTACAAGTATTAGAGG	628	0.13963499235120422	No Hit
GTACATGGGAGAAATCGTAAATAGA	613	0.13629976164217866	No Hit
GTGTATATCAATGAGTTACAATGAA	610	0.13563271550037354	No Hit
GTATCAACGCAGAGTACATGGGAGA	599	0.1331868796470881	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	581	0.12918460279625743	No Hit
GATTAAAGATAAGAGACAGTTGGAC	566	0.12584937208723185	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	562	0.1249599772314917	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	549	0.12206944395033618	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	542	0.12051300295279092	No Hit
GTTCTACAAATCCCGTTTCCAACGA	534	0.11873421324131062	No Hit
GTTCTACAGTGTGGTTTTTATCATT	520	0.11562133124622008	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	510	0.11339784410686969	No Hit
ATTTAGAAATGTCCACTGTAGGACG	504	0.11206375182325945	No Hit
TACTAACAGTGTTGCATCTATAAAG	503	0.11184140310932442	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	500	0.1111743569675193	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	492	0.109395567256039	No Hit
GATATACACTGTTCTACAATGCCGG	490	0.10895086982816891	No Hit
CTGAAGGACCTGGAATATGGCGAGA	487	0.10828382368636379	No Hit
CATTTACACCTACTACCCAACTATC	479	0.10650503397488349	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	478	0.10628268526094846	No Hit
GATATATTTTGATCAACGGACCAAG	477	0.10606033654701343	No Hit
ATCGTAAATAGATAGAAACCGACCT	474	0.1053932904052083	No Hit
GTTAGTATGAGTAACAAGAATTCCA	473	0.10517094169127325	No Hit
ACCTTTGCACGGTCAGGATACCGCG	471	0.10472624426340318	No Hit
GTTTAAAATTGAACTTAAATTCATT	461	0.1025027571240528	No Hit
GTATTGGAATTAGTGAAATTGGAGT	455	0.10116866484044255	No Hit
GATAGAAACCGACCTGGATTGCTCC	454	0.10094631612650752	No Hit
GAATATGGCAAGAAAACTGAAAATC	453	0.10072396741257249	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.446974278700772E-4	0.0
8	0.0	0.0	0.0	4.446974278700772E-4	0.0
9	0.0	0.0	0.0	6.670461418051159E-4	0.0
10	0.0	0.0	0.0	6.670461418051159E-4	0.0
11	0.0	0.0	0.0	6.670461418051159E-4	0.0
12	0.0	0.0	0.0	6.670461418051159E-4	0.0
13	0.0	0.0	0.0	6.670461418051159E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTCCC	30	7.702848E-4	19.003006	13
TACGACC	30	7.708619E-4	19.000893	4
TTAGCCT	35	9.916062E-5	19.00089	4
GGTCTAC	25	0.006035549	18.994549	1
AAACTTG	50	4.54572E-6	17.100801	6
TAGCCTA	50	4.54572E-6	17.100801	5
AACTTGC	40	2.7546522E-4	16.625778	7
CGTCCCC	35	0.0021646812	16.288292	14
ACTGTGT	50	8.688502E-5	15.200712	8
CACCTTT	120	3.6379788E-12	14.2522545	14
GTCCCCA	40	0.005265844	14.2522545	15
CCACCTT	120	3.6379788E-12	14.2522545	13
TTGAGAT	40	0.0052697356	14.250668	9
GTTCGCA	40	0.0052697356	14.250668	9
CGTGTGC	40	0.0052697356	14.250668	8
CTCTGCC	40	0.0052697356	14.250668	9
ATAAAGG	40	0.0052697356	14.250668	3
TATACCA	40	0.0052697356	14.250668	5
GGGATGC	40	0.0052697356	14.250668	7
GTACGAC	40	0.0052697356	14.250668	3
>>END_MODULE