FastQCFastQC Report
Thu 2 Feb 2017
SRR4062040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449744
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG16140.3588708242911523No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT9370.20834074495713117No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG8140.1809918531431214No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7880.1752107865808104No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC7680.17076381230210963No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA7600.16898502259062934No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC7560.16809562773488917No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC7500.16676153545127895No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT7360.1636486534561884No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC7300.16231456117257817No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG7290.16209221245864314No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT7180.15964637660535771No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA7140.15875698174961755No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA7080.15742288946600733No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6770.15053007933402113No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC6610.1469724999110605No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6540.14541605891351525No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6500.1445266640577751No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG6180.13741150521185386No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA6170.1371891564979188No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC6030.13407627450282827No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5990.1331868796470881No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA5950.13229748479134795No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC5940.13207513607741292No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA5890.13096339250773772No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT5790.12873990536838736No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT5700.126738766942972No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT5440.120957700380661No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC5280.11740012095770037No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5260.1169554235298303No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG5160.11473193639047992No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC5100.11339784410686969No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA5060.11250844925112953No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT5040.11206375182325945No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC4970.11050731082571418No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT4840.10761677754455869No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC4730.10517094169127325No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA4700.10450389554946815No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA4650.10339215197979294No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA4650.10339215197979294No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA4600.10228040841011776No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA4590.1020580596961827No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA4550.10116866484044255No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC4530.10072396741257249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC250.0023518135.19822311
GGTCGGT401.8181732E-532.9983311
CGATCGT300.00574264529.33185217
TGGACCT554.9539085E-627.9985855
GTAATAC554.9539085E-627.9985853
TATTGCG554.9539085E-627.99858516
GGTTTGA407.027296E-427.4986115
GTTACTG407.027296E-427.4986123
TAGGACC3850.026.2843864
CTGCATC858.210918E-925.88104629
GGACCTG6650.024.8107766
AGGACCT6350.024.5971815
AACTAGC450.001395451824.44864742
CGTTTAT1350.024.44864536
TCGGCCT450.001396356424.44592943
ACTGTTG450.001397261424.4432126
AGGGGTC1001.7389539E-924.1987785
GGGCGTT1102.0008883E-1023.99878733
CTGTAGG9650.023.7165571
TGCGAGA752.0603784E-623.46547919
Produced by FastQC (version 0.11.5)