##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449744
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27040049450354	32.0	32.0	32.0	32.0	32.0
2	31.496538030524032	32.0	32.0	32.0	32.0	32.0
3	31.60579574157743	32.0	32.0	32.0	32.0	32.0
4	31.684969671635418	32.0	32.0	32.0	32.0	32.0
5	31.656144384360882	32.0	32.0	32.0	32.0	32.0
6	35.275868049379206	36.0	36.0	36.0	36.0	36.0
7	35.297696022626205	36.0	36.0	36.0	36.0	36.0
8	35.25273933615568	36.0	36.0	36.0	36.0	36.0
9	35.34088948379522	36.0	36.0	36.0	36.0	36.0
10	35.229819630723256	36.0	36.0	36.0	36.0	36.0
11	35.351064160944894	36.0	36.0	36.0	36.0	36.0
12	35.265762300330856	36.0	36.0	36.0	36.0	36.0
13	35.321347255327474	36.0	36.0	36.0	36.0	36.0
14	35.27998594756128	36.0	36.0	36.0	36.0	36.0
15	35.27002027820271	36.0	36.0	36.0	36.0	36.0
16	35.28139341492049	36.0	36.0	36.0	36.0	36.0
17	35.272675121847094	36.0	36.0	36.0	36.0	36.0
18	35.26631594862855	36.0	36.0	36.0	36.0	36.0
19	35.23161176135757	36.0	36.0	36.0	36.0	36.0
20	35.22375618129425	36.0	36.0	36.0	36.0	36.0
21	35.2064685687858	36.0	36.0	36.0	36.0	36.0
22	35.18722206410758	36.0	36.0	36.0	36.0	36.0
23	35.17840149062578	36.0	36.0	36.0	36.0	36.0
24	35.15291810452168	36.0	36.0	36.0	36.0	36.0
25	35.142427692198225	36.0	36.0	36.0	36.0	36.0
26	35.126145095876765	36.0	36.0	36.0	36.0	36.0
27	35.07649907502935	36.0	36.0	36.0	36.0	36.0
28	35.049757195204386	36.0	36.0	36.0	36.0	36.0
29	35.01410580241204	36.0	36.0	36.0	36.0	36.0
30	34.973569408374544	36.0	36.0	36.0	36.0	36.0
31	34.97816313280444	36.0	36.0	36.0	36.0	36.0
32	34.93075171653207	36.0	36.0	36.0	36.0	36.0
33	34.95128339677683	36.0	36.0	36.0	36.0	36.0
34	34.93206579743143	36.0	36.0	36.0	36.0	36.0
35	34.92424356967519	36.0	36.0	36.0	36.0	36.0
36	34.872200629691555	36.0	36.0	36.0	36.0	36.0
37	34.79952817602903	36.0	36.0	36.0	32.0	36.0
38	34.77991701945996	36.0	36.0	36.0	32.0	36.0
39	34.66630349710057	36.0	36.0	36.0	32.0	36.0
40	34.7229623963855	36.0	36.0	36.0	32.0	36.0
41	34.69733893059163	36.0	36.0	36.0	32.0	36.0
42	34.690254900565655	36.0	36.0	36.0	32.0	36.0
43	34.69282747518589	36.0	36.0	36.0	32.0	36.0
44	34.60738998185634	36.0	36.0	36.0	32.0	36.0
45	34.58053025721299	36.0	36.0	36.0	32.0	36.0
46	34.43547662670319	36.0	36.0	36.0	32.0	36.0
47	34.45461418051158	36.0	36.0	36.0	32.0	36.0
48	34.38113237753033	36.0	36.0	36.0	32.0	36.0
49	34.363982621224515	36.0	36.0	36.0	32.0	36.0
50	33.99590656017646	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	6.0
22	14.0
23	58.0
24	151.0
25	349.0
26	864.0
27	1744.0
28	3165.0
29	5555.0
30	8623.0
31	12825.0
32	20062.0
33	34118.0
34	77153.0
35	285054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.26819432488762	22.962251182778477	13.58721642788666	25.182338064447247
2	12.60609214034292	22.79516239852177	43.296488758735855	21.302256702399454
3	16.558308726742325	27.946342808353197	32.30637873990537	23.18896972499911
4	9.670612615176633	19.65962858870824	43.288181721156924	27.381577074958198
5	9.813138140808993	41.563645095876765	37.8908890390978	10.732327724216443
6	26.48195417837703	43.57745739798641	19.214041765982426	10.726546657654131
7	24.795883880607636	34.31707816001992	23.42154647977516	17.46549147959728
8	22.59329752036714	40.14083567540646	20.56080792628695	16.70505887793945
9	24.945524565085915	15.8378988935928	22.400965882813335	36.81561065850795
10	13.838761606602867	28.568029812515565	36.26974456579743	21.323464015084138
11	31.273346614963177	24.241568536767584	28.208714290796543	16.276370557472696
12	21.615630225194778	27.639501583122843	33.01678288092781	17.72808531075456
13	26.15865914831549	23.18541214557615	29.181712262976273	21.474216443132093
14	18.862730797965064	25.814463339144044	28.571587391938525	26.751218470952367
15	21.708794336333558	34.18722651108186	24.16730406631328	19.9366750862713
16	20.577752182913354	29.292951752445283	29.90885905950732	20.22043700513404
17	17.841927852289302	30.772839659895407	29.92569105980291	21.45954142801238
18	17.720303105766835	30.058877939449996	33.09927425379772	19.12154470098545
19	22.34159877619268	28.3734746878224	28.507550962325233	20.777375573659683
20	20.78475929586453	31.221831134670246	29.494177785979993	18.499231783485236
21	21.312791276815254	27.78358354975275	28.201821480664556	22.70180369276744
22	20.925237468426484	30.858443914760397	28.279865879255755	19.936452737557367
23	20.783156640221993	30.55226973567185	29.098998541392433	19.56557508271372
24	22.050393784825832	29.999688707647564	28.929065366946976	19.020852140579628
25	21.018050348866684	29.80219770446167	30.43811785423643	18.74163409243522
26	17.69766288747129	30.596362811650206	32.49611444758451	19.20985985329399
27	18.65873914097225	31.640210699046783	29.769133790337104	19.93191636964386
28	18.62462144626427	30.261841338734996	30.096189336902818	21.017347878097915
29	19.789702586360242	29.081655341705503	28.553132448681918	22.575509623252337
30	19.946991830871923	29.39818829462225	29.70836612991448	20.94645374459134
31	21.280550713294673	27.599256465900602	30.18695079867658	20.933242022128145
32	18.749193982327643	30.908609825188666	29.92649118828128	20.415705004202408
33	19.657849798996764	31.077457397986407	30.16271478885766	19.101978014159165
34	19.59665584559968	30.231022368479564	31.861964690710188	18.310357095210566
35	19.792815468355332	31.437884663275106	29.44230495570814	19.326994912661423
36	18.370350935316104	30.0137190071619	30.52823736328242	21.087692694239575
37	20.574292473806853	28.990341000053366	27.940614049131046	22.494752477008735
38	20.27624665642378	31.30743557987562	28.81201041483692	19.604307348863685
39	20.501623160048027	30.010005781117975	28.1273624760973	21.361008582736694
40	21.786394037496887	29.95948806432104	30.039311252623712	18.21480664555836
41	21.21261982002815	28.20248278438287	31.40724467855658	19.177652717032398
42	20.30570064551133	31.876207693214237	28.72314168117566	19.09494998009877
43	18.09313733120165	31.79112475901833	30.38937301405532	19.7263648957247
44	18.503373224998775	31.171756522976622	29.766876131264482	20.55799412076012
45	18.92732625362435	31.25811587242293	30.14590782147749	19.668650052475233
46	20.75382337114828	29.04116655623102	28.360542009276596	21.84446806334411
47	21.90111954817837	30.203119614884322	28.085872791755147	19.809888045182163
48	19.506988699786092	30.78452213125325	27.82249636445302	21.885992804507634
49	20.471707343105166	29.431567525259712	29.568539205920025	20.528185925715096
50	18.807881050480617	31.228251177910643	28.104870179682795	21.858997591925945
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	65.5
2	19.0
3	55.0
4	91.0
5	82.5
6	74.0
7	57.5
8	41.0
9	64.5
10	88.0
11	138.5
12	189.0
13	375.5
14	562.0
15	798.5
16	1035.0
17	1568.0
18	2101.0
19	2242.0
20	2383.0
21	3013.0
22	3643.0
23	3994.5
24	4346.0
25	6742.5
26	9139.0
27	13394.0
28	17649.0
29	20349.5
30	23050.0
31	23681.5
32	24313.0
33	28683.5
34	33054.0
35	37959.0
36	42864.0
37	46315.0
38	49766.0
39	46586.0
40	43406.0
41	41719.5
42	40033.0
43	32828.0
44	25623.0
45	23929.5
46	22236.0
47	20289.0
48	18342.0
49	16479.5
50	14617.0
51	14607.5
52	14598.0
53	13279.5
54	11961.0
55	11014.0
56	10067.0
57	9864.0
58	9661.0
59	8389.5
60	7118.0
61	6328.0
62	5538.0
63	4670.5
64	3803.0
65	3355.5
66	2908.0
67	2442.0
68	1976.0
69	1658.0
70	1340.0
71	1057.0
72	774.0
73	638.0
74	502.0
75	400.5
76	299.0
77	252.5
78	206.0
79	154.5
80	103.0
81	81.5
82	60.0
83	44.0
84	28.0
85	20.0
86	12.0
87	10.0
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03713223522715145
2	0.0037799281368956564
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.2234871393503862E-4
17	0.0
18	0.0
19	0.0
20	2.2234871393503862E-4
21	0.0
22	0.0
23	0.0
24	0.0013340922836102315
25	4.4469742787007723E-4
26	2.2234871393503862E-4
27	6.670461418051157E-4
28	0.0013340922836102315
29	0.0
30	4.4469742787007723E-4
31	0.0
32	4.4469742787007723E-4
33	0.0
34	8.893948557401545E-4
35	0.0
36	6.670461418051157E-4
37	0.001778789711480309
38	2.2234871393503862E-4
39	8.893948557401545E-4
40	0.0
41	0.00155644099754527
42	0.005114020420505887
43	0.00466932299263581
44	0.005781066562311003
45	0.001778789711480309
46	0.002223487139350386
47	0.0020011384254153474
48	0.004002276850830695
49	0.003557579422960618
50	0.00155644099754527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	449744.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.71606861145006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.17976769784701	19.36886448987598
2	14.610569375073304	8.683373640040585
3	6.4856847830731175	5.781871620181151
4	3.4951454709078504	4.154479304819864
5	2.0693387255572318	3.074630577449466
6	1.4314109025106219	2.552154275410997
7	0.9855353723737084	2.050036572112767
8	0.7261009846623844	1.7261493343255088
9	0.5545952399352833	1.4832351181350436
>10	3.7232019954447715	21.569311319345967
>50	0.4482786444946347	9.32230899486163
>100	0.26492594349098614	15.082619297705351
>500	0.0246964862576343	4.792030206380839
>1k	7.483783714434636E-4	0.35893524935486704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1614	0.3588708242911523	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	937	0.20834074495713117	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	814	0.1809918531431214	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	788	0.1752107865808104	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	768	0.17076381230210963	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	760	0.16898502259062934	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	756	0.16809562773488917	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	750	0.16676153545127895	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	736	0.1636486534561884	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	730	0.16231456117257817	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	729	0.16209221245864314	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	718	0.15964637660535771	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	714	0.15875698174961755	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	708	0.15742288946600733	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	677	0.15053007933402113	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	661	0.1469724999110605	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	654	0.14541605891351525	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	650	0.1445266640577751	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	618	0.13741150521185386	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA	617	0.1371891564979188	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	603	0.13407627450282827	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	599	0.1331868796470881	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	595	0.13229748479134795	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	594	0.13207513607741292	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	589	0.13096339250773772	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	579	0.12873990536838736	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	570	0.126738766942972	No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT	544	0.120957700380661	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	528	0.11740012095770037	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	526	0.1169554235298303	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	516	0.11473193639047992	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	510	0.11339784410686969	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	506	0.11250844925112953	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	504	0.11206375182325945	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC	497	0.11050731082571418	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT	484	0.10761677754455869	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	473	0.10517094169127325	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA	470	0.10450389554946815	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	465	0.10339215197979294	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	465	0.10339215197979294	No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA	460	0.10228040841011776	No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA	459	0.1020580596961827	No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA	455	0.10116866484044255	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	453	0.10072396741257249	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.223487139350386E-4	0.0
7	0.0	0.0	0.0	2.223487139350386E-4	0.0
8	0.0	0.0	0.0	2.223487139350386E-4	0.0
9	0.0	0.0	0.0	2.223487139350386E-4	0.0
10	0.0	0.0	0.0	2.223487139350386E-4	0.0
11	0.0	0.0	0.0	2.223487139350386E-4	0.0
12	0.0	0.0	0.0	2.223487139350386E-4	0.0
13	0.0	0.0	0.0	2.223487139350386E-4	0.0
14	0.0	0.0	0.0	2.223487139350386E-4	0.0
15	0.0	0.0	0.0	2.223487139350386E-4	0.0
16	0.0	0.0	0.0	2.223487139350386E-4	0.0
17	0.0	0.0	0.0	2.223487139350386E-4	0.0
18	0.0	0.0	0.0	2.223487139350386E-4	0.0
19	0.0	0.0	0.0	2.223487139350386E-4	0.0
20	0.0	0.0	0.0	2.223487139350386E-4	0.0
21	0.0	0.0	0.0	2.223487139350386E-4	0.0
22	0.0	0.0	0.0	2.223487139350386E-4	0.0
23	0.0	0.0	0.0	2.223487139350386E-4	0.0
24	0.0	0.0	0.0	2.223487139350386E-4	0.0
25	0.0	0.0	0.0	2.223487139350386E-4	0.0
26	0.0	0.0	0.0	4.446974278700772E-4	0.0
27	0.0	0.0	0.0	4.446974278700772E-4	0.0
28	0.0	0.0	0.0	8.893948557401544E-4	0.0
29	0.0	0.0	0.0	0.001111743569675193	0.0
30	0.0	0.0	0.0	0.0020011384254153474	0.0
31	0.0	0.0	0.0	0.004224625564765733	0.0
32	0.0	0.0	0.0	0.006225763990181081	0.0
33	0.0	0.0	0.0	0.008893948557401544	0.0
34	0.0	0.0	0.0	0.011339784410686969	0.0
35	0.0	0.0	0.0	0.015119712547582626	0.0
36	0.0	0.0	0.0	0.021123127823828667	0.0
37	0.0	0.0	0.0	0.02868298409761998	0.0
38	0.0	0.0	0.0	0.03846632751076168	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGAC	25	0.00235181	35.198223	11
GGTCGGT	40	1.8181732E-5	32.99833	11
CGATCGT	30	0.005742645	29.331852	17
TGGACCT	55	4.9539085E-6	27.998585	5
GTAATAC	55	4.9539085E-6	27.998585	3
TATTGCG	55	4.9539085E-6	27.998585	16
GGTTTGA	40	7.027296E-4	27.49861	15
GTTACTG	40	7.027296E-4	27.49861	23
TAGGACC	385	0.0	26.284386	4
CTGCATC	85	8.210918E-9	25.881046	29
GGACCTG	665	0.0	24.810776	6
AGGACCT	635	0.0	24.597181	5
AACTAGC	45	0.0013954518	24.448647	42
CGTTTAT	135	0.0	24.448645	36
TCGGCCT	45	0.0013963564	24.445929	43
ACTGTTG	45	0.0013972614	24.44321	26
AGGGGTC	100	1.7389539E-9	24.198778	5
GGGCGTT	110	2.0008883E-10	23.998787	33
CTGTAGG	965	0.0	23.716557	1
TGCGAGA	75	2.0603784E-6	23.465479	19
>>END_MODULE