FastQCFastQC Report
Thu 2 Feb 2017
SRR4062039_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062039_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021444
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19810.1939411264836839No Hit
TATCAACGCAGAGTACTTTTTTTTT14900.14587192249403785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3950.016.1083551
TATACTG1151.8189894E-1214.8717145
TAGCGTA456.75211E-414.7799137
GTGTTAT653.3814886E-614.6103341
CGCCGGT400.00527239914.252067
CGTAGGG400.005282697714.2478692
GTATAGG1203.6379788E-1214.2450751
GTCTTAA500.00150284113.2954041
ATTATAC604.0865917E-412.6678763
GCACCGT701.088722E-412.2160516
CGTCGTG550.00306838812.09028512
ATATACT951.0367876E-612.0017344
GTATCAA10850.011.903721
TATAGGG1201.0017175E-811.8732232
GCTCTAG658.042564E-411.6882671
TGTACCG1406.7666406E-1011.5373815
TGTATAC752.0706456E-411.4010893
CTTAAAC1104.9550545E-711.2283443
GGACAGT1451.2114469E-911.1395416
TCTATAC600.005869141311.0843923
Produced by FastQC (version 0.11.5)