FastQCFastQC Report
Thu 2 Feb 2017
SRR4062039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021444
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5150.028.6362381
GTATCAA11350.025.5992051
GTATTAG901.5037585E-824.4571531
TCGTGCG500.002581182921.9985089
TACTAGC654.928817E-420.3092982
CGACCCG550.004483635519.999625
TATAGAC550.004483635519.999623
CTAATAC1404.0799932E-918.8567873
ACCGTAT708.1258523E-418.85586413
GTATAGG2250.018.5874371
CTACTAG951.589581E-518.5359481
CGCGTAA851.4313012E-418.11641710
TATAGGG1852.1827873E-1117.8392472
TCAACGC16200.017.6539864
TATGCGA750.001291271917.59880617
ATGCGAA750.001291271917.59880618
CCTATAC902.2152675E-417.1107883
CAACGCA16750.017.0743055
ATCAACG16700.016.993693
TATCAAC17150.016.9342822
Produced by FastQC (version 0.11.5)