##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062039_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1021444
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303227587611264	32.0	32.0	32.0	32.0	32.0
2	31.483677029773535	32.0	32.0	32.0	32.0	32.0
3	31.596798258152184	32.0	32.0	32.0	32.0	32.0
4	31.668057181793618	32.0	32.0	32.0	32.0	32.0
5	31.647091764208316	32.0	32.0	32.0	32.0	32.0
6	35.299123593657605	36.0	36.0	36.0	36.0	36.0
7	35.30424771206253	36.0	36.0	36.0	36.0	36.0
8	35.26272022744272	36.0	36.0	36.0	36.0	36.0
9	35.339431236563144	36.0	36.0	36.0	36.0	36.0
10	35.22965723035232	36.0	36.0	36.0	36.0	36.0
11	35.34412557125011	36.0	36.0	36.0	36.0	36.0
12	35.2782364965676	36.0	36.0	36.0	36.0	36.0
13	35.30311989693023	36.0	36.0	36.0	36.0	36.0
14	35.26577962179033	36.0	36.0	36.0	36.0	36.0
15	35.25005678235909	36.0	36.0	36.0	36.0	36.0
16	35.26664604226957	36.0	36.0	36.0	36.0	36.0
17	35.251237463825724	36.0	36.0	36.0	36.0	36.0
18	35.243898833416225	36.0	36.0	36.0	36.0	36.0
19	35.23073315815649	36.0	36.0	36.0	36.0	36.0
20	35.23705753815187	36.0	36.0	36.0	36.0	36.0
21	35.23082714275085	36.0	36.0	36.0	36.0	36.0
22	35.21812355841338	36.0	36.0	36.0	36.0	36.0
23	35.18015476129871	36.0	36.0	36.0	36.0	36.0
24	35.16916737481448	36.0	36.0	36.0	36.0	36.0
25	35.160019540963575	36.0	36.0	36.0	36.0	36.0
26	35.11831387721696	36.0	36.0	36.0	36.0	36.0
27	35.106882021921905	36.0	36.0	36.0	36.0	36.0
28	35.086191705076345	36.0	36.0	36.0	36.0	36.0
29	35.073477351670775	36.0	36.0	36.0	36.0	36.0
30	35.055633005823125	36.0	36.0	36.0	36.0	36.0
31	35.05900176612717	36.0	36.0	36.0	36.0	36.0
32	35.032818245542586	36.0	36.0	36.0	36.0	36.0
33	35.02105059112394	36.0	36.0	36.0	36.0	36.0
34	35.00108963389084	36.0	36.0	36.0	36.0	36.0
35	34.997855976441194	36.0	36.0	36.0	36.0	36.0
36	34.970700302708714	36.0	36.0	36.0	36.0	36.0
37	34.96196267245194	36.0	36.0	36.0	36.0	36.0
38	34.953582379454964	36.0	36.0	36.0	36.0	36.0
39	34.93058258700428	36.0	36.0	36.0	36.0	36.0
40	34.91770767658335	36.0	36.0	36.0	36.0	36.0
41	34.902062178641216	36.0	36.0	36.0	36.0	36.0
42	34.884083708945376	36.0	36.0	36.0	36.0	36.0
43	34.86537783764945	36.0	36.0	36.0	32.0	36.0
44	34.83346419382756	36.0	36.0	36.0	32.0	36.0
45	34.814648673838214	36.0	36.0	36.0	32.0	36.0
46	34.799480930917404	36.0	36.0	36.0	32.0	36.0
47	34.76490341124917	36.0	36.0	36.0	32.0	36.0
48	34.74587153089156	36.0	36.0	36.0	32.0	36.0
49	34.73248362122642	36.0	36.0	36.0	32.0	36.0
50	34.33187918280395	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	24.0
22	55.0
23	168.0
24	424.0
25	997.0
26	2256.0
27	4166.0
28	6980.0
29	11664.0
30	17618.0
31	25848.0
32	39713.0
33	66783.0
34	155692.0
35	689053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.960058212530576	17.66335058947043	12.210724659822974	26.16586653817602
2	16.280367573198408	19.3305867816981	37.472317461685016	26.916728183418474
3	19.069469640379896	22.58039882949447	28.142943435682405	30.207188094443232
4	13.16696085831515	15.525780684776342	34.373920032738	36.933338424170515
5	14.859258069948034	36.373114923578775	33.30931504810836	15.458311958364826
6	35.82611641189612	34.128418451561615	15.997971495199922	14.047493641342337
7	31.38194555942372	29.649300402175744	19.61468274325367	19.35407129514687
8	28.372382626947733	33.91414507305344	18.33786286864478	19.375609431354043
9	27.047983051444817	14.91486562161019	18.28489863369896	39.752252693246035
10	16.804413377192983	26.991306390977442	31.074855106516292	25.129425125313283
11	36.896589534032216	21.453745873488906	21.669420937418007	19.980243655060875
12	24.2893394057824	23.540987073202253	28.551051256848147	23.6186222641672
13	30.030133810566216	19.552320048871987	24.6752636463673	25.74228249419449
14	23.410975051006222	18.93916847130141	25.2077451137801	32.44211136391226
15	25.25336680229166	27.079017547706975	21.89645247316544	25.77116317683593
16	25.789034674644963	25.86461439656641	23.269449540745498	25.076901388043133
17	24.318317989042963	26.19203793844792	24.83875768030357	24.650886392205546
18	24.766457615801972	25.72010941394617	25.458812149882228	24.054620820369635
19	25.84977737399211	25.049733514514745	24.913847455171307	24.186641656321832
20	25.75855921475795	24.791985455869785	25.145896540482436	24.303558788889834
21	25.956782750694114	24.540258692595973	24.860393717129867	24.642564839580043
22	25.951006614165827	24.379995379090776	24.974252137170517	24.69474586957288
23	25.127099603208404	24.853662906300205	25.029198888239478	24.990038602251914
24	24.821255669741767	24.95156305222771	25.16342268836564	25.063758589664893
25	25.226934523809526	24.712758458646615	25.18816572681704	24.87214129072682
26	24.91514422300304	24.933158025099882	25.337391660197035	24.814306091700043
27	25.139655506626905	25.101767892841686	24.837631505264127	24.92094509526728
28	25.281835546082988	24.52054915221714	25.122744703743482	25.074870597956394
29	25.05759032110581	25.003157310421862	25.043590464041415	24.895661904430906
30	25.063048245614034	25.00264332706767	24.977776472431078	24.956531954887218
31	25.272506906902205	24.599438832552412	24.903714552563923	25.224339707981464
32	25.17578090581746	24.979196077701914	24.989867266080697	24.855155750399923
33	25.182290952807985	24.824366289292414	24.966909590736254	25.026433167163347
34	25.58322131815997	24.708964509872835	25.09312841980774	24.614685752159453
35	25.392239598528356	24.8516313212106	25.12741790527509	24.628711174985952
36	25.055069206083164	24.86288991445427	25.10705453662567	24.974986342836893
37	25.58523402018153	24.626087569473746	25.015248245350385	24.773430164994338
38	25.20471019458727	24.627879746711518	25.177787524328306	24.989622534372906
39	25.26761291434233	24.990063028788825	24.859560736921868	24.882763319946978
40	25.548341367710808	24.725388763358538	24.804492463610337	24.921777405320313
41	25.012066404877082	24.892332423825007	25.159017104434856	24.93658406686306
42	25.394439902681164	24.849395673521737	24.94877054196018	24.807393881836916
43	25.19529395178621	24.496357482653863	25.043934408253698	25.26441415730623
44	24.87355565607138	24.94904043281855	24.98281766754977	25.194586243560295
45	25.132316572907754	24.918789051256397	25.065056872139994	24.883837503695858
46	25.26330938632612	24.662774054210697	24.904691694519993	25.169224864943185
47	25.221870970710587	25.036639916508964	25.0386958637279	24.70279324905255
48	25.202124930732197	25.184012500514	24.466662358845426	25.147200209908384
49	25.256729198057187	24.91758916866636	24.81214638239213	25.013535250884317
50	25.047458207895833	25.11647942824975	24.851751229899406	24.984311133955014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	14.0
2	25.0
3	63.0
4	101.0
5	125.0
6	149.0
7	132.5
8	116.0
9	157.0
10	198.0
11	296.0
12	394.0
13	755.5
14	1117.0
15	1420.0
16	1723.0
17	1715.0
18	1707.0
19	1649.0
20	1591.0
21	1812.5
22	2034.0
23	2639.0
24	3244.0
25	4221.5
26	5199.0
27	6400.0
28	7601.0
29	9181.5
30	10762.0
31	13195.5
32	15629.0
33	18062.5
34	20496.0
35	23741.5
36	26987.0
37	30305.5
38	33624.0
39	38227.5
40	42831.0
41	46590.5
42	50350.0
43	53351.0
44	56352.0
45	60955.5
46	65559.0
47	69445.0
48	73331.0
49	77129.5
50	80928.0
51	82217.5
52	83507.0
53	84586.0
54	85665.0
55	83573.5
56	81482.0
57	77079.5
58	72677.0
59	66247.0
60	59817.0
61	52879.5
62	45942.0
63	39253.0
64	32564.0
65	26731.5
66	20899.0
67	17412.0
68	13925.0
69	11479.5
70	9034.0
71	7073.0
72	5112.0
73	4223.5
74	3335.0
75	2722.5
76	2110.0
77	1792.0
78	1474.0
79	1133.0
80	792.0
81	641.0
82	490.0
83	387.5
84	285.0
85	228.0
86	171.0
87	115.5
88	60.0
89	37.5
90	15.0
91	16.0
92	17.0
93	10.0
94	3.0
95	4.0
96	5.0
97	7.5
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03504842164621849
2	0.0041118260031876444
3	4.895030956175766E-4
4	9.790061912351535E-5
5	0.0
6	1.958012382470307E-4
7	0.0
8	0.0
9	0.0
10	3.916024764940614E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.874037147410921E-4
17	0.0
18	1.958012382470307E-4
19	0.0
20	9.790061912351535E-5
21	0.0
22	0.0
23	9.790061912351535E-5
24	0.0012727080486056995
25	3.916024764940614E-4
26	4.895030956175766E-4
27	1.958012382470307E-4
28	0.00146850928685273
29	4.895030956175766E-4
30	3.916024764940614E-4
31	1.958012382470307E-4
32	1.958012382470307E-4
33	0.0
34	4.895030956175766E-4
35	1.958012382470307E-4
36	1.958012382470307E-4
37	0.00146850928685273
38	0.0
39	5.874037147410921E-4
40	0.0
41	0.0012727080486056995
42	0.0057761365282874046
43	0.0034265216693230367
44	0.004503428479681705
45	0.00293701857370546
46	0.0021538136207173374
47	0.0016643105250997608
48	0.004503428479681705
49	0.0036223229075700676
50	0.0018601117633467914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1021444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.214497517397646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.86864090014838	27.97359510384525
2	17.395685636910105	15.730743700870036
3	7.718083890048024	10.469078546569293
4	4.238657598871547	7.665950939251048
5	2.493367200670678	5.636817255234254
6	1.6089268248791162	4.364809075750276
7	1.0803942443140808	3.4194638014144787
8	0.755509372639609	2.7327981322867405
9	0.5302434929511972	2.1577223786092343
>10	2.222192040876168	16.48486280346505
>50	0.07042561354045339	2.118558641160139
>100	0.01765521854226371	1.1848915559267676
>500	2.1796560834456212E-4	0.06070806561741935
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9580123824703067E-4
2	0.0	0.0	0.0	0.0	1.9580123824703067E-4
3	0.0	0.0	0.0	0.0	1.9580123824703067E-4
4	0.0	0.0	0.0	0.0	1.9580123824703067E-4
5	0.0	0.0	0.0	0.0	2.9370185737054603E-4
6	0.0	0.0	0.0	0.0	2.9370185737054603E-4
7	0.0	0.0	0.0	0.0	2.9370185737054603E-4
8	0.0	0.0	0.0	0.0	2.9370185737054603E-4
9	0.0	0.0	0.0	0.0	2.9370185737054603E-4
10	0.0	0.0	0.0	0.0	2.9370185737054603E-4
11	0.0	0.0	0.0	0.0	2.9370185737054603E-4
12	0.0	0.0	0.0	0.0	0.001762211144223276
13	0.0	0.0	0.0	0.0	0.0021538136207173374
14	0.0	0.0	0.0	0.0	0.0021538136207173374
15	0.0	0.0	0.0	9.790061912351533E-5	0.0025454160972113987
16	0.0	0.0	0.0	9.790061912351533E-5	0.0026433167163349143
17	0.0	0.0	0.0	9.790061912351533E-5	0.0026433167163349143
18	0.0	0.0	0.0	9.790061912351533E-5	0.0026433167163349143
19	0.0	0.0	0.0	9.790061912351533E-5	0.0027412173354584295
20	0.0	0.0	0.0	2.9370185737054603E-4	0.003230720431076006
21	0.0	0.0	0.0	2.9370185737054603E-4	0.003230720431076006
22	0.0	0.0	0.0	3.9160247649406134E-4	0.0033286210501995215
23	0.0	0.0	0.0	6.853043338646074E-4	0.0033286210501995215
24	0.0	0.0	0.0	8.81105572111638E-4	0.0033286210501995215
25	0.0	0.0	0.0	0.0014685092868527302	0.0033286210501995215
26	0.0	0.0	0.0	0.0021538136207173374	0.0036223229075700676
27	0.0	0.0	0.0	0.0027412173354584295	0.003818124145817098
28	0.0	0.0	0.0	0.0031328198119524907	0.003818124145817098
29	0.0	0.0	0.0	0.003916024764940613	0.003818124145817098
30	0.0	0.0	0.0	0.005874037147410921	0.003818124145817098
31	0.0	0.0	0.0	0.009300558816733957	0.003916024764940613
32	0.0	0.0	0.0	0.014097689153786208	0.004013925384064129
33	0.0	0.0	0.0	0.01928642196733252	0.00420972662231116
34	0.0	0.0	0.0	0.025552061591237504	0.00440552786055819
35	0.0	0.0	0.0	0.03152399935777194	0.00440552786055819
36	0.0	0.0	0.0	0.03994345260239426	0.00440552786055819
37	0.0	0.0	0.0	0.05531384980478617	0.00440552786055819
38	0.0	0.0	0.0	0.07440447053387166	0.004503428479681705
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	515	0.0	28.636238	1
GTATCAA	1135	0.0	25.599205	1
GTATTAG	90	1.5037585E-8	24.457153	1
TCGTGCG	50	0.0025811829	21.998508	9
TACTAGC	65	4.928817E-4	20.309298	2
CGACCCG	55	0.0044836355	19.99962	5
TATAGAC	55	0.0044836355	19.99962	3
CTAATAC	140	4.0799932E-9	18.856787	3
ACCGTAT	70	8.1258523E-4	18.855864	13
GTATAGG	225	0.0	18.587437	1
CTACTAG	95	1.589581E-5	18.535948	1
CGCGTAA	85	1.4313012E-4	18.116417	10
TATAGGG	185	2.1827873E-11	17.839247	2
TCAACGC	1620	0.0	17.653986	4
TATGCGA	75	0.0012912719	17.598806	17
ATGCGAA	75	0.0012912719	17.598806	18
CCTATAC	90	2.2152675E-4	17.110788	3
CAACGCA	1675	0.0	17.074305	5
ATCAACG	1670	0.0	16.99369	3
TATCAAC	1715	0.0	16.934282	2
>>END_MODULE