FastQCFastQC Report
Thu 2 Feb 2017
SRR4062038_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062038_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421642
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19980.47386171206853206No Hit
TATCAACGCAGAGTACTTTTTTTTT12130.2876848131827474No Hit
CCATAGGGTCTTCTCGTCTTATTAT9780.2319503275290412No Hit
GGTATCAACGCAGAGTACTTTTTTT7930.1880742430782512No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7260.17218398546634348No Hit
GATATACACTGTTCTACAAATCCCG5590.13257692544860333No Hit
GTTCATGCTAGTCCCTAATTAAGGA5350.12688489287120353No Hit
GTCAGGATACCGCGGCCGTTAAACT5270.12498754867873695No Hit
CTGTAGGACGTGGAATATGGCAAGA4910.11644949981263726No Hit
GTATCAACGCAGAGTACATGGGGTG4830.11455215562017067No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4800.11384065154799569No Hit
TTGTAGAACAGTGTATATCAATGAG4650.11028313118712083No Hit
ATCCTGACCGTGCAAAGGTAGCATA4510.1069627788503043No Hit
GTATTAGAGGCACTGCCTGCCCAGT4490.10648844280218762No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4320.10245658639319612No Hit
GGTCAGGATACCGCGGCCGTTAAAC4300.10198225034507948No Hit
CTTCTACACCATTGGGATGTCCTGA4220.10008490615261288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGC250.00601861819.00344711
GCGGCGC250.00601861819.00344719
GTTTAAA453.5523597E-516.8759061
CCAGACA402.758802E-416.622094
GTCCTGG402.771332E-416.612221
CCAGGAC753.272362E-916.4618283
TAGCCTG350.002162322616.2906065
TCCTGGT350.002172647716.2789962
CCCTTAT456.787312E-414.7664181
TATGTCA400.00526430214.25258410
TAGCATA759.604864E-713.93586219
CGTCTTA2050.013.90165615
TAGGACA551.9577179E-413.81576254
TTGTAGA1101.8735591E-1013.807561
AAACTTT852.685083E-713.4110117
TCTCGTC2150.012.814754512
TTATCCT751.4751806E-512.6644494
GATATAC1600.012.4591661
GTAGCAT853.921303E-612.29488718
CTTCTAG853.9579445E-612.2846681
Produced by FastQC (version 0.11.5)