##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062038_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421642
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246192267373743	32.0	32.0	32.0	32.0	32.0
2	30.92867171676446	32.0	32.0	32.0	32.0	32.0
3	30.97657965762424	32.0	32.0	32.0	32.0	32.0
4	30.942353940072383	32.0	32.0	32.0	32.0	32.0
5	30.963988407226985	32.0	32.0	32.0	32.0	32.0
6	34.59629021776768	36.0	36.0	36.0	32.0	36.0
7	34.5183971236262	36.0	36.0	36.0	32.0	36.0
8	34.4403214101062	36.0	36.0	36.0	32.0	36.0
9	34.519554503583606	36.0	36.0	36.0	32.0	36.0
10	34.3218227785657	36.0	36.0	36.0	32.0	36.0
11	34.55958846604465	36.0	36.0	36.0	32.0	36.0
12	34.43581996100958	36.0	36.0	36.0	32.0	36.0
13	34.48650751111132	36.0	36.0	36.0	32.0	36.0
14	34.41792326191413	36.0	36.0	36.0	32.0	36.0
15	34.38484306591848	36.0	36.0	36.0	32.0	36.0
16	34.38319237647104	36.0	36.0	36.0	32.0	36.0
17	34.33987600855702	36.0	36.0	36.0	32.0	36.0
18	34.37053471902704	36.0	36.0	36.0	32.0	36.0
19	34.336726417197525	36.0	36.0	36.0	32.0	36.0
20	34.26736188520119	36.0	36.0	36.0	32.0	36.0
21	34.237379103599736	36.0	36.0	36.0	32.0	36.0
22	34.23356544177288	36.0	36.0	36.0	32.0	36.0
23	34.237324554954206	36.0	36.0	36.0	32.0	36.0
24	34.192682417785704	36.0	36.0	36.0	32.0	36.0
25	33.86707443755603	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	8.0
5	32.0
6	86.0
7	19.0
8	73.0
9	55.0
10	33.0
11	2.0
12	17.0
13	24.0
14	87.0
15	127.0
16	157.0
17	250.0
18	368.0
19	518.0
20	720.0
21	929.0
22	1304.0
23	1720.0
24	2256.0
25	3047.0
26	4175.0
27	5311.0
28	6831.0
29	9137.0
30	11970.0
31	16707.0
32	24225.0
33	35624.0
34	84550.0
35	211278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.8409136725967	20.84721764352918	12.843688558221606	23.468180125652513
2	15.572926795717695	20.44222349765915	40.67147010971392	23.313379596909225
3	18.875433428675784	24.76769171659101	29.764157879508012	26.592716975225194
4	11.75821803924545	16.96169144906464	38.41576079866604	32.864329713023864
5	12.65507154228124	38.48004384352039	34.87149435704474	13.993390257153635
6	30.24279574638749	39.232005088007824	17.299938535726405	13.225260629878283
7	28.234470151114067	32.7201718877981	19.99097826474045	19.054379696347386
8	25.972450404079733	35.577534876212	19.159599623935193	19.29041509577307
9	25.454748353978353	15.788986316591755	21.337379234365468	37.41888609506443
10	16.298498672505428	27.315753727196302	31.414363538782315	24.971384061515955
11	34.08153329137524	24.22391374048521	22.9012836802793	18.793269287860255
12	23.106917850671696	26.631742179392493	29.62296277122482	20.63837719871099
13	27.402621149192736	22.016418396012472	26.94146226784735	23.639498186947446
14	21.370449780487746	22.93089816008605	26.813736314616026	28.88491574481018
15	24.613981676300302	29.317207084403957	23.44013450678471	22.62867673251103
16	23.158717079877377	29.11740471537495	25.429258690247497	22.29461951450017
17	20.144330778217878	28.864255929482614	27.099659001320305	23.891754290979208
18	20.411651997597232	28.904675187152318	28.928892803806438	21.754780011444012
19	23.481918815684207	27.161777518225993	27.670514838369662	21.68578882772013
20	23.348415000640934	27.709596403188513	27.7520877743542	21.18990082181635
21	24.523213802467847	25.809598868152055	27.007914389756397	22.659272939623698
22	22.971676835782624	27.54041926829847	27.562023693644498	21.9258802022744
23	23.033358493432267	27.58353864923499	27.62246559475151	21.760637262581238
24	22.651815663345605	28.02034891311831	27.292279424855963	22.035555998680124
25	22.78372540176134	27.7498991145821	27.72687350155482	21.73950198210174
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	247.0
2	258.5
3	270.0
4	270.0
5	270.0
6	677.0
7	1084.0
8	1084.0
9	1084.0
10	1180.0
11	1276.0
12	1276.0
13	1276.0
14	1642.0
15	2008.0
16	2008.0
17	2008.0
18	3590.0
19	5172.0
20	5172.0
21	5172.0
22	7939.5
23	10707.0
24	10707.0
25	10707.0
26	16568.5
27	22430.0
28	22430.0
29	22430.0
30	28049.5
31	33669.0
32	33669.0
33	33669.0
34	36609.5
35	39550.0
36	39550.0
37	39550.0
38	41288.0
39	43026.0
40	43026.0
41	43026.0
42	43846.5
43	44667.0
44	44667.0
45	44667.0
46	49284.0
47	53901.0
48	53901.0
49	53901.0
50	52140.5
51	50380.0
52	50380.0
53	50380.0
54	45049.5
55	39719.0
56	39719.0
57	39719.0
58	34232.5
59	28746.0
60	28746.0
61	28746.0
62	23905.0
63	19064.0
64	19064.0
65	19064.0
66	15535.5
67	12007.0
68	12007.0
69	12007.0
70	9638.5
71	7270.0
72	7270.0
73	7270.0
74	5532.5
75	3795.0
76	3795.0
77	3795.0
78	2719.5
79	1644.0
80	1644.0
81	1644.0
82	1126.5
83	609.0
84	609.0
85	609.0
86	400.5
87	192.0
88	192.0
89	192.0
90	120.0
91	48.0
92	48.0
93	48.0
94	36.0
95	24.0
96	24.0
97	24.0
98	80.5
99	137.0
100	137.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.115040721749731E-4
2	0.0
3	9.486720962332974E-4
4	0.00924955293827465
5	0.03391502744034038
6	0.061426518231106016
7	0.10364242651348773
8	0.10387959453754608
9	0.11265481142770407
10	0.12996807718396175
11	0.1392176301222364
12	0.12878223706367015
13	0.12546188472685357
14	0.11407781957205401
15	0.12996807718396175
16	0.12071852424568709
17	0.12475038065467862
18	0.10933445909088751
19	0.09557871369550472
20	0.09012384914216326
21	0.09107252123839656
22	0.10245658639319612
23	0.08063712817983029
24	0.09273269740680483
25	0.08822650494969667
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	421642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.342802910288846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.75038916542243	29.1108417843714
2	15.578708165669438	13.19292338911697
3	6.1593875652554235	7.824172011710829
4	2.9157521165897684	4.9384446883207245
5	1.647525831925448	3.488043079541445
6	1.000733742380228	2.5424322971569064
7	0.6822839825978815	2.0222871342792326
8	0.48867792198106763	1.6553594349643084
9	0.360055989482052	1.3721201819376805
>10	2.1001445830471104	16.80022914051455
>50	0.19182375885106828	5.576400872205719
>100	0.12003007132786088	9.736139427430693
>500	0.003365329102650305	0.9780076146398241
>1k	0.0011217763675501016	0.7625989438097318
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1998	0.47386171206853206	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1213	0.2876848131827474	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	978	0.2319503275290412	No Hit
GGTATCAACGCAGAGTACTTTTTTT	793	0.1880742430782512	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	726	0.17218398546634348	No Hit
GATATACACTGTTCTACAAATCCCG	559	0.13257692544860333	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	535	0.12688489287120353	No Hit
GTCAGGATACCGCGGCCGTTAAACT	527	0.12498754867873695	No Hit
CTGTAGGACGTGGAATATGGCAAGA	491	0.11644949981263726	No Hit
GTATCAACGCAGAGTACATGGGGTG	483	0.11455215562017067	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	480	0.11384065154799569	No Hit
TTGTAGAACAGTGTATATCAATGAG	465	0.11028313118712083	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	451	0.1069627788503043	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	449	0.10648844280218762	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	432	0.10245658639319612	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	430	0.10198225034507948	No Hit
CTTCTACACCATTGGGATGTCCTGA	422	0.10008490615261288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.3716802405832437E-4	0.0
12	0.0	0.0	0.0	2.3716802405832437E-4	0.0
13	0.0	0.0	0.0	2.3716802405832437E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCCGC	25	0.006018618	19.003447	11
GCGGCGC	25	0.006018618	19.003447	19
GTTTAAA	45	3.5523597E-5	16.875906	1
CCAGACA	40	2.758802E-4	16.62209	4
GTCCTGG	40	2.771332E-4	16.61222	1
CCAGGAC	75	3.272362E-9	16.461828	3
TAGCCTG	35	0.0021623226	16.290606	5
TCCTGGT	35	0.0021726477	16.278996	2
CCCTTAT	45	6.787312E-4	14.766418	1
TATGTCA	40	0.005264302	14.252584	10
TAGCATA	75	9.604864E-7	13.935862	19
CGTCTTA	205	0.0	13.901656	15
TAGGACA	55	1.9577179E-4	13.8157625	4
TTGTAGA	110	1.8735591E-10	13.80756	1
AAACTTT	85	2.685083E-7	13.41101	17
TCTCGTC	215	0.0	12.8147545	12
TTATCCT	75	1.4751806E-5	12.664449	4
GATATAC	160	0.0	12.459166	1
GTAGCAT	85	3.921303E-6	12.294887	18
CTTCTAG	85	3.9579445E-6	12.284668	1
>>END_MODULE