FastQCFastQC Report
Thu 2 Feb 2017
SRR4062038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421642
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG9000.21345122165249195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.12356454053438698No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT4880.11573799574046228No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC4590.10886012304277089No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT4450.10553977070595433No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA4370.10364242651348773No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC4240.10055924220072952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATT250.002351651435.198229
GTAATAC401.817913E-532.998313
AGTGCGT353.2153018E-431.4269628
CTTAAGG300.005742261729.3318332
GGATAGC804.296453E-927.50185435
CTTTAGA407.021718E-427.50185439
TGCGCTA407.026589E-427.49859210
GCTAGTT655.912316E-727.07874939
TAGGGCG508.324965E-526.40178131
TCTAGTA1251.8189894E-1224.6387372
GTACTAC450.001394228524.4518931
TACGGCG450.001397122524.4431937
ATCGTGA450.001397122524.44319315
CGCTATC450.001397122524.44319312
CTACACT901.5073965E-824.4431934
CGGTCGA450.001397122524.44319310
GCGCTAT450.001397122524.44319311
ACAGTCC651.8104776E-523.6910958
TATATTG651.8104776E-523.6910955
CTACACG651.8104776E-523.6910954