##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062038_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421642
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197330911057247	32.0	32.0	32.0	32.0	32.0
2	31.473007907181923	32.0	32.0	32.0	32.0	32.0
3	31.598548057356716	32.0	32.0	32.0	32.0	32.0
4	31.688076614758494	32.0	32.0	32.0	32.0	32.0
5	31.667224802083282	32.0	32.0	32.0	32.0	32.0
6	35.31210126125955	36.0	36.0	36.0	36.0	36.0
7	35.32049463763098	36.0	36.0	36.0	36.0	36.0
8	35.2827730634045	36.0	36.0	36.0	36.0	36.0
9	35.334032188444226	36.0	36.0	36.0	36.0	36.0
10	35.25610351909914	36.0	36.0	36.0	36.0	36.0
11	35.34561547473923	36.0	36.0	36.0	36.0	36.0
12	35.28683100829614	36.0	36.0	36.0	36.0	36.0
13	35.31615683447095	36.0	36.0	36.0	36.0	36.0
14	35.27023398997254	36.0	36.0	36.0	36.0	36.0
15	35.2669183809962	36.0	36.0	36.0	36.0	36.0
16	35.27512439462862	36.0	36.0	36.0	36.0	36.0
17	35.25956142888991	36.0	36.0	36.0	36.0	36.0
18	35.26790737165653	36.0	36.0	36.0	36.0	36.0
19	35.22726625905389	36.0	36.0	36.0	36.0	36.0
20	35.20421589879566	36.0	36.0	36.0	36.0	36.0
21	35.19344847050341	36.0	36.0	36.0	36.0	36.0
22	35.18297038720051	36.0	36.0	36.0	36.0	36.0
23	35.173521613122034	36.0	36.0	36.0	36.0	36.0
24	35.157600523667	36.0	36.0	36.0	36.0	36.0
25	35.14346056607264	36.0	36.0	36.0	36.0	36.0
26	35.11320266956328	36.0	36.0	36.0	36.0	36.0
27	35.10361633803084	36.0	36.0	36.0	36.0	36.0
28	35.06871231993018	36.0	36.0	36.0	36.0	36.0
29	35.044976544082424	36.0	36.0	36.0	36.0	36.0
30	35.02745219878475	36.0	36.0	36.0	36.0	36.0
31	35.02417928005275	36.0	36.0	36.0	36.0	36.0
32	34.98802064310481	36.0	36.0	36.0	36.0	36.0
33	34.957674994426554	36.0	36.0	36.0	36.0	36.0
34	34.927974442773724	36.0	36.0	36.0	36.0	36.0
35	34.92244368445269	36.0	36.0	36.0	36.0	36.0
36	34.88548341958344	36.0	36.0	36.0	36.0	36.0
37	34.863324811095666	36.0	36.0	36.0	36.0	36.0
38	34.845290554546274	36.0	36.0	36.0	36.0	36.0
39	34.8000128070733	36.0	36.0	36.0	32.0	36.0
40	34.787589945973124	36.0	36.0	36.0	32.0	36.0
41	34.74381821545292	36.0	36.0	36.0	32.0	36.0
42	34.72617291446298	36.0	36.0	36.0	32.0	36.0
43	34.71287490335403	36.0	36.0	36.0	32.0	36.0
44	34.62702719368564	36.0	36.0	36.0	32.0	36.0
45	34.57977383657226	36.0	36.0	36.0	32.0	36.0
46	34.48716446653796	36.0	36.0	36.0	32.0	36.0
47	34.46012968347556	36.0	36.0	36.0	32.0	36.0
48	34.38988525810996	36.0	36.0	36.0	32.0	36.0
49	34.349571437380526	36.0	36.0	36.0	32.0	36.0
50	33.96085067426869	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	1.0
21	7.0
22	34.0
23	73.0
24	200.0
25	467.0
26	1019.0
27	2024.0
28	3463.0
29	5612.0
30	8432.0
31	11971.0
32	17852.0
33	28955.0
34	63481.0
35	278049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.319876918405065	21.77509851509695	13.216799641288798	23.68822492520919
2	15.251447396820353	21.00806652451621	39.94625479402019	23.794231284643246
3	18.442035859975334	24.8823641020776	29.99288492552889	26.682715112418176
4	11.554826132121562	17.21673837046594	38.49924817736374	32.729187320048766
5	12.624453920624607	38.73831354561453	34.61562178340867	14.021610750352195
6	30.662030822356407	38.94061787013628	17.229071107716972	13.168280199790344
7	28.299837302735497	32.69977848506553	19.86068750266814	19.139696709530835
8	26.234340981211552	35.57757528898924	19.059533917399122	19.12854981240009
9	25.53659265443196	15.670165685581608	21.259741676588195	37.53349998339824
10	16.352774042372538	27.454161241435248	31.441439518452903	24.751625197739312
11	34.35592279706481	23.930490795508984	22.85445947035637	18.85912693706984
12	23.020714255221254	26.71104870956878	29.369939427286656	20.89829760792331
13	27.596871279426622	21.683086599532306	27.156450258750315	23.56359186229076
14	21.557387546781392	22.677769292432917	26.910269849777773	28.854573311007915
15	24.71433111502175	29.24115719022298	23.51236356909416	22.532148125661106
16	23.376340005692057	28.948866331467606	25.138269613888625	22.536524048951712
17	20.269090840096574	28.696856575009132	27.240644907291017	23.793407677603277
18	20.587942320462954	28.474053695095343	29.146665401764537	21.791338582677167
19	23.593475033322107	26.848843331546668	27.873883531526744	21.68379810360448
20	23.44986244189356	27.469879518072286	27.8299022863106	21.250355753723554
21	24.451548944365125	25.613672262250915	27.33835813320305	22.596420660180915
22	22.919680676972405	27.363260775729177	27.725653516490294	21.991405030808124
23	22.974940826577996	27.370612984474977	27.751030495064533	21.90341569388249
24	22.776938456539632	27.83531805795032	27.404141477147405	21.98360200836264
25	22.814669351415066	27.405304512606698	28.030480906742937	21.7495452292353
26	21.17574791645914	27.58859495586261	29.366660500239544	21.8689966274387
27	21.755918005298398	28.07154021112948	28.39338103629425	21.779160747277874
28	21.455388928285974	27.66078556469726	28.159086219532153	22.724739287484613
29	21.781903476670802	27.13790707216363	27.600150839936532	23.480038611229038
30	22.118235246683142	27.42449612697268	28.2811632837959	22.176105342548276
31	22.523823754025965	26.94728653489486	27.971387778141438	22.557501932937736
32	21.114370892497575	27.78148027805909	28.34144062309522	22.762708206348115
33	22.102452086016303	27.804459243764246	28.32812748285864	21.76496118736081
34	21.99920310787927	26.848386530625795	29.380890716681137	21.771519644813797
35	22.28241019632769	27.70573140246939	27.878152555959794	22.13370584524312
36	21.703957385649435	27.73703758164509	27.665887174427596	22.893117858277876
37	22.538925848186228	27.311537230068662	26.80802115573897	23.341515766006143
38	22.13963504584458	28.079508208385313	27.978474630136468	21.80238211563364
39	22.238745084646073	27.338854657312673	27.626067859158805	22.796332398882456
40	22.84995327789926	27.793720739395035	27.954283491682517	21.40204249102319
41	22.73229642489529	27.318220843472364	28.641630972540426	21.30785175909192
42	21.978848201812536	28.79803803908268	27.421439735875282	21.801674023229502
43	21.370598587373642	28.383686015568298	27.96459421382932	22.281121183228738
44	21.438566698606355	27.82168167199522	28.174836585805497	22.564915043592933
45	22.03783376103445	28.006043155912476	27.913545871537877	22.042577211515198
46	23.472601700060718	27.639268366727382	26.38177367941712	22.506356253794777
47	22.767526800113842	28.459349207855045	26.51385067830377	22.259273313727352
48	22.21218179963902	28.491161661081186	26.03046754374106	23.26618899553873
49	22.284494515268307	27.768751852949897	27.48413874888823	22.46261488289357
50	21.620038327261685	28.114564359441406	27.029257741347905	23.236139571949
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	61.0
2	22.0
3	40.5
4	59.0
5	69.5
6	80.0
7	71.0
8	62.0
9	92.5
10	123.0
11	247.5
12	372.0
13	688.0
14	1004.0
15	1396.5
16	1789.0
17	2213.5
18	2638.0
19	2715.0
20	2792.0
21	3161.0
22	3530.0
23	3538.5
24	3547.0
25	4625.0
26	5703.0
27	7796.5
28	9890.0
29	11619.0
30	13348.0
31	13846.5
32	14345.0
33	16228.5
34	18112.0
35	20589.5
36	23067.0
37	26357.0
38	29647.0
39	29687.5
40	29728.0
41	29695.5
42	29663.0
43	26863.0
44	24063.0
45	23951.0
46	23839.0
47	23942.5
48	24046.0
49	23677.5
50	23309.0
51	23634.0
52	23959.0
53	22781.0
54	21603.0
55	20556.5
56	19510.0
57	18614.5
58	17719.0
59	15711.0
60	13703.0
61	12532.5
62	11362.0
63	9846.5
64	8331.0
65	7338.5
66	6346.0
67	5487.0
68	4628.0
69	4055.5
70	3483.0
71	2996.5
72	2510.0
73	2082.5
74	1655.0
75	1320.5
76	986.0
77	733.0
78	480.0
79	368.0
80	256.0
81	175.5
82	95.0
83	91.0
84	87.0
85	52.0
86	17.0
87	13.5
88	10.0
89	6.0
90	2.0
91	5.5
92	9.0
93	6.5
94	4.0
95	4.0
96	4.0
97	3.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03154334719975714
2	0.0054548645533414606
3	4.743360481166487E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.3716802405832435E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.743360481166487E-4
17	0.0
18	4.743360481166487E-4
19	0.0
20	4.743360481166487E-4
21	0.0
22	0.0
23	0.0
24	0.0011858401202916218
25	2.3716802405832435E-4
26	9.486720962332974E-4
27	0.0011858401202916218
28	0.0021345122165249194
29	7.115040721749731E-4
30	0.0018973441924665948
31	9.486720962332974E-4
32	0.0011858401202916218
33	2.3716802405832435E-4
34	9.486720962332974E-4
35	0.0
36	0.0
37	0.0016601761684082704
38	0.0
39	9.486720962332974E-4
40	0.0
41	9.486720962332974E-4
42	0.0054548645533414606
43	0.0037946883849331895
44	0.0033203523368165407
45	0.0018973441924665948
46	0.0023716802405832436
47	4.743360481166487E-4
48	0.0030831843127582166
49	0.004031856408991514
50	0.0023716802405832436
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	421642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.77529138102569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.64919964256909	32.578616077363996
2	15.61899774892791	14.611663415713746
3	6.070428859113655	8.518382360784855
4	2.8388006675837842	5.3114291359552706
5	1.5200898876957885	3.5551323711160565
6	0.9623413231752656	2.7008277479714917
7	0.6025395810998386	1.972877513218228
8	0.44734823312093597	1.673987516241503
9	0.32672199241921374	1.3754264756398191
>10	1.739724478392312	15.478063402058378
>50	0.14242300921050652	4.685441379743685
>100	0.08036726948303761	7.200062308117556
>500	0.0010173072086460458	0.33809029607544266
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	900	0.21345122165249195	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	521	0.12356454053438698	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	488	0.11573799574046228	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	459	0.10886012304277089	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	445	0.10553977070595433	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	437	0.10364242651348773	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	424	0.10055924220072952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3716802405832437E-4	0.0
10	0.0	0.0	0.0	2.3716802405832437E-4	0.0
11	0.0	0.0	0.0	2.3716802405832437E-4	0.0
12	0.0	0.0	0.0	2.3716802405832437E-4	0.0
13	0.0	0.0	0.0	2.3716802405832437E-4	0.0
14	0.0	0.0	0.0	2.3716802405832437E-4	0.0
15	0.0	0.0	0.0	2.3716802405832437E-4	0.0
16	0.0	0.0	0.0	2.3716802405832437E-4	0.0
17	0.0	0.0	0.0	2.3716802405832437E-4	0.0
18	0.0	0.0	0.0	2.3716802405832437E-4	0.0
19	0.0	0.0	0.0	2.3716802405832437E-4	0.0
20	0.0	0.0	0.0	2.3716802405832437E-4	0.0
21	0.0	0.0	0.0	4.7433604811664875E-4	0.0
22	0.0	0.0	0.0	4.7433604811664875E-4	0.0
23	0.0	0.0	0.0	4.7433604811664875E-4	0.0
24	0.0	0.0	0.0	0.0016601761684082706	0.0
25	0.0	0.0	0.0	0.0023716802405832436	0.0
26	0.0	0.0	0.0	0.0026088482646415678	0.0
27	0.0	0.0	0.0	0.0028460162886998924	0.0
28	0.0	0.0	0.0	0.004980528505224811	0.0
29	0.0	0.0	0.0	0.00758937676986638	0.0
30	0.0	0.0	0.0	0.01138406515479957	0.0
31	0.0	0.0	0.0	0.028697330911057247	0.0
32	0.0	0.0	0.0	0.04008139606585682	0.0
33	0.0	0.0	0.0	0.05099112517253974	0.0
34	0.0	0.0	0.0	0.06877872697691406	0.0
35	0.0	0.0	0.0	0.08704066482940503	0.0
36	0.0	0.0	0.0	0.10980879513900418	0.0
37	0.0	0.0	0.0	0.14277515048311126	0.0
38	0.0	0.0	0.0	0.18546539481360966	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTATT	25	0.0023516514	35.1982	29
GTAATAC	40	1.817913E-5	32.99831	3
AGTGCGT	35	3.2153018E-4	31.426962	8
CTTAAGG	30	0.0057422617	29.331833	2
GGATAGC	80	4.296453E-9	27.501854	35
CTTTAGA	40	7.021718E-4	27.501854	39
TGCGCTA	40	7.026589E-4	27.498592	10
GCTAGTT	65	5.912316E-7	27.078749	39
TAGGGCG	50	8.324965E-5	26.401781	31
TCTAGTA	125	1.8189894E-12	24.638737	2
GTACTAC	45	0.0013942285	24.451893	1
TACGGCG	45	0.0013971225	24.443193	7
ATCGTGA	45	0.0013971225	24.443193	15
CGCTATC	45	0.0013971225	24.443193	12
CTACACT	90	1.5073965E-8	24.443193	4
CGGTCGA	45	0.0013971225	24.443193	10
GCGCTAT	45	0.0013971225	24.443193	11
ACAGTCC	65	1.8104776E-5	23.691095	8
TATATTG	65	1.8104776E-5	23.691095	5
CTACACG	65	1.8104776E-5	23.691095	4
>>END_MODULE