FastQCFastQC Report
Thu 2 Feb 2017
SRR4062037_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062037_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623871
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25020.154076278226534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.014.2452141
CGCGTAA1001.0053554E-813.30168310
CGCGCCA655.446302E-513.15551110
CGAACGA655.4526085E-513.15388916
CTTATAG1251.03682396E-1012.9180513
GCGTAAC1051.9901563E-812.6682711
TATACCG751.477222E-512.667885
TACACCG604.088892E-412.6678795
GTATCAA16750.012.4734231
TCGCGTA1155.313268E-912.3928729
TCTTATA1251.8371793E-811.3975772
GGCCTAA752.080401E-411.3961721
CCGCCCA855.3160988E-511.1778869
CAAGACG1701.0913936E-1111.1751294
CCTAACC855.3311473E-511.1747843
AACCGCT700.001490271710.8581837
TCTATAC1408.36917E-910.8555043
GGTTTAA700.001495647510.8534971
GTCTAGC1158.853749E-710.7355241
GGACCGT803.7675287E-410.6885236
Produced by FastQC (version 0.11.5)